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Articles by Avinash Marwal
Total Records ( 7 ) for Avinash Marwal
  Rajneesh Prajapat , Avinash Marwal , Anurag Sahu and Rajarshi Kumar Gaur
  Begomovirus associated symptoms were observed in several Mimosa pudica plants growing in crop fields of Lakshmangarh, Rajasthan (India). Amplification of a PCR product was found up to the expected size (~570 bp) during agarose gel electrophoresis only in infected samples not in healthy samples. The PCR product was cloned and partially sequenced (GenBank accession HQ876467) and sequence analysis of HQ876467 by using BLASTn revealed the highest 98% nucleotide sequence identities with Ageratum yellow vein china virus (AJ558120). GeneBank-NCBI generated CDC for HQ876467 from 1 to 774 (ADW83735) and it was utilized for in silico analysis. In Neighbor-Joining tree, the coat protein of mimosa yellow vein virus (ADW83735) place in monophyletic clusters of 74 bootstrap values with AV1 protein of Ageratum yellow vein China virus (CAD90081). The closest homologue of coat protein was 2B7R|A, with highest sequence identity only 37% that was selected as representative model using homology modeling softwares. Homology modeling study of coat protein (ADW83735) and docking between α-lactalbumin and coat protein was carried out by using modeling and docking software (Hex 6.3). The ADW83735 model was validated by using Procheck server for reliability that results shown only 49.2% of residues present in core region. On the basis of RMS and energy values, the best docking orientation -1.00 was selected. This study will be used for the screening of inhibitors against mimosa yellow vein virus proteins and can be further applied in future antiviral agent designing.
  Avinash Marwal , Rajneesh Prajapat , Anurag Kumar Sahu and R.K. Gaur
  Geminivirus are a large diverse family of plant viruses that infect a broad variety of plants and cause significant loss to Agricultural crops and ornamental plants in India. The vector white fly is the carrier of these viruses. This review focuses on the current status of Geminivirus in India and research related to it. Researches being carried out on Geminivirus include their molecular characterization, serological and computational characterization. Many costly measures in controlling the infections and increasing the crop yield have been applied. A cost effective appraise against these viruses is the relevance of RNAi Technology. RNAi is a challenging approach in which an interference RNA molecule put a check halt on the multiplication of Begomovirus in plants through its ability to form a Dicer complex.
  Rajneesh Prajapat , Avinash Marwal , Vivek Bajpai and Rajarshi Kumar Gaur
  The uncharacterized alphasatellite of begomovirus associated with a common weed Verbesina encelioides was characterized by using molecular and in silico tools and techniques. Verbesina encelioides leaf curl alphasatellite (HQ631431) shows 87 % nucleotide sequence identity with Sida yellow vein disease associated DNA1 (FN806782). Translated 3-frame of HQ631431 (HQ631431/3-f.pdb) and its homologous 2HWT.pdb had respectively 67 and 77.2% residues in most favorable region of its Ramachandran plot therefore both of these two models cannot be placed in good quality. The 3d2GO server showed the hydrolase activity as possible function for HQ631431/3-f.pdb with 0.58% confidence level. In silico prediction of results can be used to confirm the begomovirus not only in host weeds but also in other crops also.
  Avinash Marwal , Rajneesh Prajapat and R.K. Gaur
  The aim of this study were to present and highlight the in silico recombination analysis approach for in depth study about the nature of the virus. To detect the possibility of recombination in betasatellite and alphasatellite, Recombination Detection Program (RDP) was utilized, which is based on a pair wise scanning approach. The betasatellite and alphasatellite genomes were isolated from an ornamental plant Marigold and is identified as a new recombinant species, sharing nucleotide identity with other isolates reported from China and Pakistan. One factor favoring the spread of viruses among these plants is that many dicotyledonous species in India are hosts of whiteflies of the B. tabaci complex, which are the known or likely vectors of all the viruses. The present study remarkably suggests that the exchange of DNA with other viruses would create a new disease complex posing a serious threat to agriculture crops and horticulture ornamental plants production.
  Avinash Marwal , Surendra Meena , Subhash Chandra and Anima Sharma
  Dermatophytes as the name suggest are the fungus that feed on skin. The chief source of their growth is keratin which is widely available in skin, nails and hairs. Here we have evaluated an In vitro study of antidermatophytic activity of Mint (Mentha piperita) against two dermatophytes i.e., Trichophyton rubrum and Microsporum canis. The data in the manuscript is very much helpful in curing dermatophytic infections as an application from Biotechnological point of view. Distribution of Trichophyton rubrum and Microsporum canis was found to be 19.23 and 32.69%, respectively. At variable temperature Trichophyton rubrum and Microsporum canis showed maximum growth at 37°C (0.23 and 0.19 g dry weight of mycelium, respectively). At variable pH Trichophyton rubrum showed maximum growth at pH 7.5 and 8.0 (0.32 g dry weight of mycelium) and Microsporum canis showed maximum growth at pH 7.5 (0.39 g dry weight of mycelium). Mentha piperita highest keratinase activity against Microsporum canis was found to be 2.99 unit mL-1 with 2.91 mg mL-1 extracellular release of protein and in Trichophyton rubrum it was found to be 2.99 unit mL-1 with 2.75 mg mL-1 extracellular release of protein.
  Rajneesh Prajapat , Avinash Marwal , Zuber Shaikh and Rajarshi Kumar Gaur
  Geminivirus Database (GVDB) is an online interactive database of Geminiviridae family. GVDB comprises of partial and complete nucleotide sequences along with duly annotated expressed genes of isolated Begomovirus species. The in silico homology modeling, docking and recombination results obtained for different begomoviral sequences are also mentioned. This database is endowed with comprehensive information about Geminivirus members which grounds infection in various plants species in India assorting from crops, ornamentals plants and common weeds. The home page of this database offers various links associated with current research projects and also the publications related to molecular and in silico study of Begomovirus infection. The main feature of GVDB includes flexible database designs based on platform of PHP allows easy retrieval of the information. The database is made available at www.wikigeminivirus.org.
  Avinash Marwal , Rajneesh Prajapat and R.K. Gaur
  Earlier reported the molecular characterization of complete genome of a begomovirus and its satellite molecules isolated from an ornamental plant Marigold i.e., Ageratum enation virus (AEV: KC589699), Ageratum leaf curl betasatellite (ALCB: KC589700) and Marigold leaf curl alphasatellite (MLCuA: KC206078). It presented and highlighted the computational approach for prediction of binding sites in protein molecules of the three begomovirus components for in depth study using two servers. Thus, in order to take a step forward to find a cure against such viruses that causes major crop loss worldwide.
 
 
 
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