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Articles by Astuti Kusumorini
Total Records ( 2 ) for Astuti Kusumorini
  Anggraini Barlian , Kusnandar Anggadiredja , Astuti Kusumorini and Utami Ekawati
  Frog skin histology has been largely explored, particularly in relation to the skin-derived secretions, among which a number of peptides have shown potential medicinal applications. Duttaphrynus melanostictus is a frog species ubiquitous in Indonesia; however, there is only limited information with regard to skin structural characteristics as well as its potential use. The present work explored the structure of the frog skin and further assessed antifungal activity of its extract. Structural studies were carried out by Scanning Electron Microscopic (SEM) assays while antifungal evaluations were performed by testing the activity of the lyophilisate of skin extract against the pathogenic fungi Candida albicans, Microsporum gypseum and Trichophyton mentagrophytes. Results of the structural studies revealed features common to frog species, in particular an extensive distribution of secretory glands. In addition, we observed a flattened, irregular-shaped structure, believed to be secretory ‘vesicle’ of the mucous gland, considered distinct to D. melanostictus. Antifungal studies demonstrated equipotent activity of the lyophilisate against all test fungi. Taken together, results of the present work shed some light on the structural characteristic of D. melanostictus frog skin and further open an opportunity for the development of alternative antifungal agents.
  Anggraini Barlian , Kusnandar Anggadiredja and Astuti Kusumorini
  The treatment of fungal infection poses particular challenge for the medical field. Thus, the development of antifungal agents has only been progressed slowly, partly due to the considerably high toxicity of the agents. We have previously demonstrated the antifungal effect of the skin extract from Duttaphrynus melanostictus frog. In the current study, the antifungal effect was particularly investigated in the lyophilisate of granular gland secretion of the frog skin against the pathogenic fungi Candida albicans, Mycrosporum gypseum and Trychophyton mentagrophytes. Antifungal test of the lyophilisate was performed by agar diffusion method, while the study of its mechanism of action was carried out under Scanning Electron Microscope (SEM). The lyophilisate was shown to be equipotent against all test fungi in vitro. Mean while, SEM assay revealed that the lyophilisate induced detrimental effect on cellular membrane integrity as indicated by the formation of pores in all test fungi and the shrinkage of hyphal and microconidial structures in M. gypseum and T. mentagrophytes, possibly due to the leakage of essential intracellular components. The results open an opportunity for developing effective alternative antifungal agents, particularly in the face of the emergence of dug-resistant fungi.
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