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Articles by Asna Urooj
Total Records ( 2 ) for Asna Urooj
  Lohith Mysuru Shivanna and Asna Urooj
  Background and Objective: Annona reticulata or custard apple belongs to family Annonaceae. It is traditionally used to treat various human ailments. However, there are no studies on the cytotoxicity or apoptosis-inducing properties of the leaf extracts on HT-29 cell line. Hence, the present study aimed at screening the anti-cancer potential of Annona reticulata leaf extract through various in vitro studies. Materials and Methods: The methanolic leaf extract of Annona reticulata (ARM) was subjected to quantification of flavonoids by UPLC/MS; further analyzed for its cytotoxic effect by MTT assay, cell cycle analysis, apoptotic potential by Annexin V-FITC staining assay and morphological study by phase contrast microscopy. Results: The ARM showed significant dose-dependent cytotoxicity towards HT-29 cell lines with IC50 of 76.76 μg mL1. In cell cycle analysis, ARM 160 μg mL1 exhibited significant (p<0.001) increase in the percentage of cells at S phase, indicating the induction of apoptosis. Further, apoptosis induction was confirmed by Annexin V-FITC assay and morphological evaluation. The results showed that the percentage of late apoptotic cells were found to be higher in ARM 160 μg mL1 treated cells (82.53%) compared to untreated (0.71%) cells. Also, ARM 160 μg mL1 showed similar activity as colchicine treated cells (82.18%). Under morphological evaluation, the formation of apoptotic bodies was found to be more evident in ARM compared to colchicine. Conclusion: These findings suggest ARM as a potent anti-cancer agent and also provide a basis for further studies validating ARM as an adjuvant in cancer therapy.
  Faiyaz Ahmed and Asna Urooj
  In the present study, the hepatoprotective effects of petroleum ether (FRPE) and methanol (FRME) extract of Ficus racemosa Linn. (Moraceae) stem bark were studied using the model of hepatotoxicity induced by carbon tetrachloride (CCl4) in rats. CCl4 administration induced a significant decrease in serum total protein, albumin, urea and a significant increase (P ≤ 0.01) in total bilirubin associated with a marked elevation in the activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP) as compared to control rats. Further, CCl4 intoxication caused significant increase in the TBARS and decrease in glutathione (GSH) levels in serum, liver and kidney. Pretreatment with FRPE and FRME restored total protein and albumin to near normal levels. Both the extracts resulted in significant decreases in the activities of AST, ALT and ALP, compared to CCl4-treated rats. However, a greater degree of reduction was observed in FRME pretreated group (FRPE 43%, 38%, and 33%; FRME 55%, 73%, and 38%). Total bilirubin content decreased from 2.1 mg/dL in CCl4-treated rats to 0.8 and 0.3 mg/dL in FRPE and FRME pretreated rats, respectively. The extracts improved the antioxidant status considerably as reflected by low TBARS and high GSH values. FRME exhibited higher hepatoprotective activity than a standard liver tonic (Liv52), while the protective effect of FRPE was similar to that of Liv52. The protective effect of F. racemosa was confirmed by histopathological profiles of the liver. The results indicate that F. racemosa possesses potent hepatoprotective effects against CCl4-induced hepatic damage in rats.
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