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Articles by Asmah Awal
Total Records ( 4 ) for Asmah Awal
  Nurul Izzati Osman , Asmah Awal , Norrizah Jaafar Sidik and Shamsiah Abdullah
  Lycium barbarum or commonly known as goji or wolfberry is a type of Chinese medicinal herbs which has been consumed since ancient times. In the present study, the best explant and corresponding treatment for callus induction and somatic embryogenesis in this species were determined. Leaves and nodes were used as explants and cultured on different combinations and concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP) plant growth regulators. For callus induction, the optimal callus formation effects were found in the treatment of 0.3 mg L-1 2,4-D and 0.1 mg L-1 or 0.3 mg L-1 BAP in Murashige and Skoog’s (MS) media, while 0.1 mg L-1 BAP with either 0.3 mg L-1 or 0.5 mg L-1 2,4-D in MS media were the optimal treatments in nodal explant. As for somatic embryogenesis, after a series of sequential subculture on MS basal media, the treatment of 1.0 mg L-1 2,4-D and 0.1 mg L-1 BAP in MS media during callus induction phase in leaf explant were identified to be the optimum treatment to produce somatic embryos. Through this study, reliable protocols useful for L. barbarum mass propagation through callus culture and somatic embryos formation had been established and verified.
  Asmah Awal , Rosna Mat Taha and Nor Azlina Hasbullah
  Direct somatic embryogenesis induction of Begonia x hiemalis Fotsch. (Elatior Begonia) was initiated from two different explants i.e., leaves and petioles. Both explants were cultured on MS medium supplemented with different concentrations of Benzylaminopurine (BAP) and 2,4-Dichlorophenoxyacetic acid (2,4-D). The results showed that combinations of 0.5-1.0 mg L-1 BAP and 0.1 mg L-1 2,4-D produced direct somatic embryogenesis from leaf and petiole explants. Different concentrations of casein hydrolysate were also tested to optimize somatic embryo induction. The results showed that 100 mg L-1 casein hydrolysate could produce 53.08% nodular callus and 24.16% green embryogenic callus, whereas 500 mg L-1 casein hydrolysate produced 30.83% nodular callus and 23.75% green embryogenic callus. The embryogenic callus were then transferred to MS medium supplemented with 0.5 mg L-1 Gibberelic Acid (GA3) with 0.2 g L-1 activated charcoal for further embryogenesis development and further regeneration.
  Nurul Izzati Osman , Norrizah Jaafar Sidik and Asmah Awal
  The present study was conducted to initiate and establish a homogeneous cell suspension culture system in Barringtonia racemosa from endosperm-derived friable calli which are useful for plant secondary metabolites study. Initiation and establishment of cell suspension cultures study were carried out by using different treatments of liquid media involving different concentrations and combinations of 2, 4-D (0.5 and 1.0 mg/L) and kinetin (0.75, 1.5 and 2.0 mg/L) plant hormones in MS (Murashige and Skoog’s) medium. The difference in the treatments was formulated based on the reference treatments which produced the most optimum friable calli suitable for cell suspension cultures initiation. The treatment consisted of 1.0 mg/L 2, 4-D and 1.5 mg/L kinetin (treatment A) was found to be the most optimum treatment by considering timely mean dry cell biomass changes at every 5 days, interval with its maximum record of 16.32±0.72 g/L. The distinctive phases of cell growth involving lag, exponential and declining phases had been identified. However, no lag phase were identified from the optimum treatment while other two treatments (1.0 mg/L 2,4-D + 2.0 mg/L kinetin (treatment B) and 0.5 mg/L 2,4-D+0.75 mg/L kinetin (treatment C)) underwent lag phase during first 10 days in culture. These were followed by exponential phases which were continued and achieved the peak of highest dry cell biomass records on day 50 (treatment A and C) and 55 (treatment B). Afterwards, all treatments had shown a declining progress in mean dry cell biomass records. The findings pertaining to cell growth phases are providing a useful information on plant cellular and molecular processes studies in this species and would further be utilised in research related to plant secondary metabolites production.
  Nor Azlina Hasbullah , Rosna Mat Taha and Asmah Awal
  Regeneration potentials in Gerbera jamesonii Bolus ex. Hook f. from tissues culture system was studied using leaf, petiole and root explants. In vitro regeneration, callus induction and root formation were optimized by manipulation of growth regulators during organogenesis. Various kinds of plant growth regulators such as 6-Benzylaminopurine (BAP), α-Naphthalene acetic acid (NAA), 2, 4-Dichlorophenoxyacetic acid (2,4-D), Indole-3-acetic acid (IAA), Indole-3-Butyric acid (IBA), N6-[2-Isopentenyl]adenine (2iP), Kinetin and Zeatin were used to initiate cultures. These plant growth regulators were added to Murashige and Skoog medium in different combinations and concentrations. Adventitious shoots were obtained from petiole explants cultured on Murashige and Skoog (MS) medium supplemented with 2.0 mg L-1 BAP and 0.5 mg L-1 NAA. Effectiveness of shoot regeneration medium, type of growth regulator used and duration of induction period were investigated. Leaf explants cultured on MS medium supplemented with 1.0 mg L-1 BAP and 2.0 mg L-1 2, 4-D showed the best results for callus induction. Root explants were found to be non-regenerative in all experiments conducted. Petiole segment was identified as the best explant for regeneration of this species. Regenerated plants were rooted on Murashige and Skoog basal medium. Plantlets were then transferred to field with 75% survival rate.
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