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Articles by Ashok Pandey
Total Records ( 2 ) for Ashok Pandey
  Umaiyal Munusamy , Vikineswary Sabaratnam , Sekaran Muniandy , Noorlidah Abdullah , Ashok Pandey and E.B.G. Jones
  Four strains of Pycnoporus sanguineus isolated from Thailand (KUM 60953), Shah Alam (KUM 60954), Endau Rompin (KUM 60955), Cherating (KUM 60956) and Gombak (KUM 60957) were grown on PDA plates. Laccase of P. sanguineus was produced using oil palm frond parenchyma tissue (OPFPt) in solid substrate fermentation (SSF). Strain KUM 60954 produced significant (p = 0) levels of laccase at 2.53 U mL-1 (76 U g-1) followed by KUM 60957 at 1.05 U mL-1 (32 U g-1), KUM 60956 at 0.55 U mL-1 (17 U g-1) and KUM 60955 at 0.46 U mL-1 (14 U g-1). Meanwhile, KUM 60953 (reference strain) from Thailand produced 2.44 U mL-1 of laccase (73 U g-1 of OPFPt). Biodegradation of a mixture of 10 ppm each of phenanthrene, anthracene and pyrene by 30 U mL-1 of laccase in sodium citrate buffer pH 5 was studied. The reaction mixture was incubated at 40°C and was shaken at 80 rpm for 24 h. Laccase of KUM 60954 degrades 90% of phenanthrene, 37% of anthracene and 96% of pyrene. Meanwhile, laccase of KUM 60953 degrades 89% of phenanthrene, 43% of anthracene and 95% pyrene. Pyrene was rapidly biodegraded followed by phenanthrene and anthracene. However, a similar pattern of degradation was observed for both KUM 60953 and KUM 60954. Degraded PAH sample was further tested for toxicity using Artemia. The untreated PAH caused more than 50% of Artemia death while for the treated PAH no death was observed indicating that the toxicity level was reduced and possibly no any new toxic compound was produced during the degradation.
  Umaiyal Munusamy , Vikineswary Sabaratnam , Sekaran Muniandy , Noorlidah Abdullah , Ashok Pandey and E.B.G. Jones
  Four strains of Pycnoporus sanguineus isolated from Shah Alam (KUM 60954), Endau Rompin (KUM 60955), Cherating (KUM 60956), Gombak (KUM 60957) and the standard strain from Thailand (KUM 60953) were evaluated for their ability to grow on Polycyclic Aromatic Hydrocarbon (PAH)- and acetonitrile-incorporated Potato Dextrose Agar (PDA). The mycelial growth was then compared to the growth of P. sanguineus on PAH and acetonitrile free PDA. All strains tested showed a high tolerance to PAH up to 25 ppm and 2.5% of acetonitrile. Laccase derived from the best strains KUM 60954 and KUM 60953 which exhibited higher tolerance to both PAH and acetonitrile was further characterised. The activity was maximum for both strains at pH 5.0 and 40 °C. Laccase from both strains was stable over a wide range of pH (3-5) and at temperatures below 60 °C. Laccase was stored as a concentrate or as a lyophilised powder to minimise the degradation of enzyme activity during storage. The laccase activity in the extract remained a high activity up to four months at optimum pH 5.0 and at both 0 and 4 °C. Further laccase was also stable up to 24 h at 40 °C when mixed with 1% of acetonitrile.
 
 
 
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