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Articles by Asep Gunawan
Total Records ( 4 ) for Asep Gunawan
  Isyana Khaerunnisa , Muhammad Pramujo , Irma Isnafia Arief , Cahyo Budiman , Asep Gunawan , Jakaria and Cece Sumantri
  Myostatin, or growth and differentiation factor-8 (GDF-8), is a member of the Transforming Growth Factor (TGF)-β superfamily. This family functions as a negative regulator of skeletal muscle. Mutations in exon 2 have been reported to convert Thymine into Guanine (T4842G) that alters the amino acid leucine into arginine, which is associated with body weight in chickens. The objectives of this study were to identify the polymorphism of T4842G mutation in the myostatin gene in Indonesian chickens and evaluate their effects on carcass characteristics. The gene polymorphism was identified with the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method using the BsrI restriction enzyme. The effect of genotype on carcass and meat quality was analyzed using the SAS General Linear Model (GLM) procedure. Genotyping was performed on 332 chickens from 7 Indonesian chicken populations (Kampung, Merawang, Sentul, Cobb broiler, F1 crossbreed of Kampung x layer, F1 crossbreed of Kampung x Cobb broiler and F2 crossbreed of Kampung x Cobb broiler). The product of amplification was 247 bp. The myostatin|BsrI locus was polymorphic in all populations, producing two alleles (G and T) and three genotypes (GG, GT, TT). Results from the analysis of the allele and genotype frequency showed that the T allele had a higher frequency than the G allele in all populations, except for the F1 crossbreed of the Kampung x Cobb broiler chicken population, which had equal allele frequencies. A significant effect was found between genotype and carcass characteristics in the F2 crossbreed Kampung x Cobb broiler chickens. A SNP in the coding region of myostatin in exon 2 was associated with live weight, carcass weight, breast weight, thighs weight, drum sticks weight, wings weight, breast muscle weight, thighs muscle weight, drum sticks muscle weight and free water. Here, the association of myostatin|BsrI gene polymorphism with chicken carcass characteristics in Indonesian chickens has been demonstrated, providing evidence that myostatin might be an important candidate gene for chicken carcass characteristics.
  Anggraeni , Asep Gunawan , Rukmiasih , Tuti Suryati and Cece Sumantri
  Background and Objective: Flavin containing monooxygenase 3 (FMO3) is an excellent candidate gene that affects fishy odor and fatty acid composition. It has been reported that down regulation of FMO3 can inhibit fatty acid oxidation. The aim of this study was to investigate the association and expression of the FMO3 gene as a candidate gene for fatty acid composition in Indonesian Cihateup ducks. Methodology: A total of one hundred Indonesian Cihateup ducks were used in this study. Tissues from breast muscles were used for genomic DNA isolation and fatty acid composition analysis. Results: Association analysis showed that the SNP g.849A>G was significantly associated with unsaturated fatty acids (palmitoleic, oleic, linoleic, linolenic and arachidonic acid) and saturated fatty acids (lauric, palmitic and arachidic acid). Compared to the GG genotype, the AG genotype ducks exhibited greater levels (p<0.05) of lauric acid (C14:0), palmitic acid (C16:0), arachidonic acid (C20:4n6) palmitoleic acid (C16:1), oleic acid (C18:1), linolenic acid (C18:3) and linoleic acid (C18:2, p<0.05) but not pentadecanoic acid (C15:0). Furthermore, to analyze the mRNA expression of FMO3 in liver tissues, the ducks were divided into two groups according to the genotypes AG and GG, where AG had relatively favourable unsaturated fatty acid composition. FMO3 mRNA expression was higher (p<0.01) in animals with the AG genotype. Conclusion: These results will improve the understanding of functions of the FMO3 gene in maintaining muscular fatty acid composition and will shed light on FMO3 as a candidate gene in the selection of ducks with unsaturated fatty acids for meat quality improvement.
  Asep Gunawan , Eva Siti Nurajizah , Kasita Listyarini , Ahmad Furqon , Woki Bilyaro , Cece Sumantri , Jakaria , Syeda Hasina Akter and Muhammad Jasim Uddin
  Background and Objective: The stearoyl-CoA desaturase (SCD) gene encodes an enzyme involved in fatty acid (FA) biosynthesis. The aim of the present study was to investigate the association and expression of SCD as a candidate gene for FA composition in Indonesian crossbred chickens. Materials and Methods: Sixty-two Indonesian crossbred, unsexed chickens (F2 Kampung×broilers) were used. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken. Results: A single nucleotide polymorphism in coding region c.17492542 C>G of SCD was associated with FA composition, including both unsaturated [linoleic (C18:2n6c) and eicosadienoic (C20:2) acids] and saturated [lauric acid (C12:0)] forms. SCD mRNA expression analysis in liver revealed 6 chickens with extremely high and low FA compositions, of them, high FA birds (n = 3) had higher unsaturated and lower saturated FAs, while the low FA group (n = 3) had lower unsaturated and higher saturated FA levels. SCD expression was higher (p<0.05) in tissues collected from high FA chickens than low FA chickens. Conclusion:These results will improve the understanding of SCD function in FA composition and will shed light on SCD as a potential candidate in the selection of chickens with higher levels of unsaturated and lower levels of saturated FA.
  Asep Gunawan , Anggraeni , Kasita Listyarini and Cece Sumantri
  Background and Objective: The fat mass and obesity-associated gene (FTO) regulates glucose metabolism, body weight and fat content, it is also involved in DNA repair, fatty acid (FA) metabolism and post translational modification. Due to these functions, FTO may affect FAs, carcass and meat quality in ducks. The objective of this study was to identify functional variants of FTO associated with fatness, carcass and meat quality and to investigate the tissue expression profile of the FTO gene in ducks. Methodology: Fifty-seven Indonesian Cihateup ducks were used in this study. Tissues from breast muscle and liver were used to evaluate genomic DNA and mRNA expression. Fatness traits, which include FA composition, carcass and meat quality, were evaluated at 12 weeks. Results: A SNP in exon 3 of the SCD gene was significantly associated with breast muscle for carcass traits and lauric acid (C12:0) for FA composition, however, there was no significant association with meat quality traits. To measure the mRNA expression of FTO, ducks were divide into three genotypes (AA, AG and GG). Compared to the AG and GG genotype, the AA genotype ducks had greater breast muscle weight and higher lauric acid levels (C12:0) for carcass and FA traits, respectively. FTO mRNA expression was significantly higher in genotype AA and GG ducks. Conclusion: The SNP of FTO in exon 3 is a functional SNP that regulates carcass and FAs specific for breast muscle weight and lauric acid levels in ducks.
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