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Articles by Arvind Sharma
Total Records ( 2 ) for Arvind Sharma
  B.P.S. Thakur , S.K. Sharma , Arvind Sharma and Adarsh Kumar
  Veterinarians working under remote field conditions are routinely presented with variety of surgical interventions in equines like castrations, management of wound, traumatic and congenital hernias and musculoskeletal disorders thus necessitating the use of general anaesthesia for management of these conditions. The present study was carried out to evaluate and recommend the suitable short term anaesthetic technique for Spiti ponies under field conditions. Seven clinically healthy male Spiti ponies presented for castration were evaluated for short term Total Intravenous Anaesthesia (TIVA) using detomidine (0.02 mg kg-1), butorphanol (0.01 mg kg-1), 5% guaifenesin (20 mg kg-1) and ketamine (2.0 mg kg-1). The studies conducted were open label trials and all the animals received same treatment. After proper tetanus prophylaxis and preanesthetic fasting, detomidine was administered intravenously. Subsequently at head down position the animals received butorphanol intravenously. Thereafter, guaifenesin was administered intravenously. As soon as the signs of ataxia developed, the induction of surgical anaesthesia was achieved by intravenous administration of ketamine hydrochloride. The onset of sedation was observed in 2.43±0.53 min following detomidine administration and the animals were ataxic in 1.43±0.43 min after butorphanol and guaifenesin administration when ketamine was injected. The ponies were in surgical plane of anaesthesia within 2.28±0.42 min following ketamine administration. During recovery the limb/head movement and sternal recumbency were attained in 18.71±1.98 and 26.14±1.62 min, respectively whereas standing ataxia and normal gait were seen at 29.42±3.21 and 71.14±4.74 min, respectively. There was excellent to good muscle relaxation. The surgical anaesthesia remained for 22.57±1.48 min. The recovery was smooth. Moderate to good suppression of palpebral and corneal reflexes were observed immediately after induction and during anaesthesia. The analgesia was excellent. A highly significant (p<0.01) to significant (p<0.05) decrease in respiration rate was observed after induction, during anaesthesia and after recovery. The mean SpO2 value in equines of this group was 76.50±4.14 and 83.33±4.18% after induction and during anaesthesia, respectively. Some of the blood biochemical parameters like plasma alanine amino transferase (ALT), total proteins and glucose showed significant increase without clinical consequence. It was concluded that detomidine (0.02 mg kg-1), butorphanol (0.01 mg kg-1), guaifenesin 5% (20 mg kg-1) and ketamine (2.0 mg kg-1) combination can safely be used for short term total intravenous anaesthesia in equines under field conditions where the monitoring facilities are meager.
  Amit Kumar , Amit Kumar Verma , Subhash Malik , Manoj Kumar Gupta , Arvind Sharma and Anu Rahal
  E. coli, often highly pathogenic in neonatal and immuno-compromised patients, are usually supposed susceptible to a variety of chemotherapeutic agents, however with the time and also due to the extensive use of chemotherapeutic agents irrespective of their susceptibility, have evolved drug resistant strains. Moreover, the production of extended spectrum β-lactamases producing enteric pathogens is a serious issue. In this context the present study was conducted to find out occurrence of extended spectrum Beta-lactamases producing alpha hemolytic Escherichia coli in neonates, of less than one month of age, suffering from diarrhea. Fecal samples were collected from various private hospitals in Mathura and Agra districts of Uttar Pradesh, India. With the help of hospital nursing staffs sterilized stool samples were collected and processed for isolation of E. coli. The double disk diffusion method was applied to assess the ESBL production. E. coli organisms were isolated from 39 kids out of 120 samples. The assessment of isolates revealed alpha hemolytic nature of 23 isolates on 5% sheep blood agar. As usual when drug sensitivity was performed that revealed their multi drug resistance pattern which on further examination with double disk method showed 17 of them to be extended spectrum β-lactamases producing E. coli. The presence of enterohemorrhagic extended spectrum β-lactamases producing Escherichia coli in kids is a matter of concern and public health importance as it may be due to the transmission from hospital itself during the birth time or post birth admission period.
 
 
 
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