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Articles by Arunrat Chaveerach
Total Records ( 7 ) for Arunrat Chaveerach
  Arunrat Chaveerach , Runglawan Sudmoon , Tawatchai Tanee , Piya Mokkamul and Alongkoad Tanomtong
  To study genetic diversity and similarity, 15 individuals of Nelumbo nucifera from NE and C Thailand were collected according to their geographical areas and the morphological differences among individual characters, such as the color of tepals, the absence or presence of petaloid staminodes and petaloid stamens. These studied samples were analyzed to determine a genetic similarity and diversity profile using sixteen inter-simple sequences repeat (ISSR) markers. A dendrogram was constructed and showed similarity values of the species between 84.10-95.00%. The samples produced 46.09% polymorphic banding profiles. The genetic diversity (H) in this population was estimated using the Shannon`s index. The genetic diversity (H) value of 1.581 reflected the broad range of distribution sites, which would require high genetic diversity to adapt to the environmental variations that can be found between NE and C. The Mantel test of 0.061 revealed that geographical distance is an important factor affecting the genetic distance among the studied samples.
  Arunrat Chaveerach , Nattapong Srisamoot , Suporn Nuchadomrong , Nison Sattayasai , Prapansak Chaveerach , Alongkoad Tanomtong and Krit Pinthong
  The genetic relationship of 20 Carnivora species in Thailand was determined based on sequence analysis of the Internal Transcribed Spacer (ITS) region of ribosomal DNA. Aligned sequences of the complete ITS region obtained from the 20 taxa and two primate outgroups resulted in 890 characters with 710 variable sites. Genetic distances and a phylogenetic tree were constructed from comparisons of ITS sequences using the Neighborjoining method. The dendogram demonstrates that the 22 taxa can be clearly grouped in six clusters: Mustelidae, Ursidae, Canidae, Felidae, Viveridae and Hylobatidae. Of these clusters, the 20 Canivora species are clustered together and the superfamilies Caniformia and Feliformia can be separated apart, whereas the outgroup Hylobatidae is segregated from the canivora. In superfamilies Caniformia, the families Mustelidae, Ursidae and Canidae are clustered together. In superfamilies Feliformia, the family Felidae is clustered with Viverridae. The phylogenetic tree of Viverridae species does not completely match the classification based on morphological characters. The Paradoxurinae, Arctictis binturong was grouped into the Viverrinae while the other Paradoxurinae, Paradoxurus hermaphroditus, Paguma larvata and Arctogalidia trivirgata are clustered together.
  Tawatchai Tanee , Arunrat Chaveerach , Nison Sattayasai , Alongkoad Tanomtong , Scott A. Suarez and Suporn Nuchadomrong
  Cytogenetic studies of three gibbon species using conventional banding patterns were investigated showing an identical number of 44 diploid chromosomes. They are assumed to have common evolutionary relationships. For in depth study, molecular markers were assessed using the Amplified Fragment Length Polymorphism (AFLP) method. With seven successful primer combinations, a total of 1669 scorable bands were generated. The resulting bands were used for dendrogram construction. From the dendrogram, the individuals of Hylobates lar are closely related to H. agilis more than H. pileatus with a bootstrap value of 78%. Averages of inter-specific genetic similarity values among all gibbon species studied are 67.15% (between H. lar and H. pileatus) to 71.03% (between H. lar and H. agilis). In summary three gibbon species show genetic stability within a species. The development of specific molecular markers of a species is beneficial for genetic differentiation of this group of primates.
  Runglawan Sudmoon , Tawatchai Tanee , Piya Mokkamul and Arunrat Chaveerach
  Nepenthes species are well known for their ornamentally attractive pitchers. The species diversity was randomly surveyed in some conservation areas of Thailand and three species were found, namely N. gracilis Korth., N. mirabilis Druce. and N. smilesii Hemsl. Young plants as unknown species from Chatuchak market were added in plant sampled set. Thirty two Inter Simple Sequence Repeat (ISSR) primers were screened and 13 successful primers were used to produce DNA banding patterns for constructing a dendrogram. The dendrogram is potentially power tool to identify unknown species from Chatuchak market, differentiate species population, population by geographical areas and sex determination. The geographical area of N. mirabilis was specified to Southern and Northeastern regions and finally, subdivided into exact areas according to province. Male and female plants of N. gracilis at Phu Wua Wildlife Sanctuary and N. mirabilis at Bung Khonglong non-hunting area were determined. Two unknown species from Chatuchak market were analyzed to be N. mirabilis with the genetic similarities (S) 77.2 to 84.7. Be more sex specific in all sample studied, 37 Random Amplified Polymorphic DNA (RAPD) primers were investigated. The result shows that only one RAPD primer show high resolution results at about 750 bp specific male-related marker.
  Arunrat Chaveerach , Tawatchai Tanee , Nison Sattayasai , Alongkoad Tanomtong , Scott A. Suareze and Suporn Nuchadomrong
  Cytogenetic studies of five langur species using conventional banding pattern were investigated. All species studied have an identical number of 44 diploid chromosomes, they are assumed to have common evolutionary relationships. For in depth study, molecular markers were assessed using the Amplified Fragment Length Polymorphism (AFLP) method. With seven successful primer combinations, a total of 1043 scorable bands were generated. The percentage of polymorphic bands for each primer ranged from 48.60 to 94.12%. The resulting bands were used for dendrogram construction. From the dendrogram, the individuals of Trachypithecus species are grouped into two major clusters, T. phayrei is clustered with T. obscurus, while T. cristatus is clustered with T. francoisi. The bootstrap value between two groups is 94%. The other cluster, Presbytis femoralis is separated from the Trachypithecus species with a bootstrap value of 94%. Averages of inter-specific genetic similarity values among all langur species studied are 70.16% (between T. obscurus and P. femoralis) to 88.12% (between T. phayrei and T. obscurus). In summary T. phayrei might be a subspecies of T. obscurus. The development of specific molecular markers of a species is beneficial for genetic differentiation of this group of primates.
  Arunrat Chaveerach , Wanpen Kakampuy , Alongkoad Tanomtong and Wiwat Sangpakdee
  Robertsonian translocation have been well documented in domestic cattle, with the most commonly occurring fusion involving chromosomes 1 and 29. The widespread nature of this translocation is indicative of its ancient origin. Fifty Giemsa’s stained metaphase spreads derived from lymphocyte cultures of the Thai gaur were analyzed for each animal. The Thai gaur had diploid chromosome number of 2n = 57 in male and 2n = 56 in female instead of the normal 2n = 58. The 2n = 57 in male chromosomes presence of an extra submetacentric chromosome and loss of two acrocentric chromosomes was observed [XY, 57, rob (1;29)]. The 2n = 56 in female chromosomes presence of two extra submetacentric chromosomes and loss of four acrocentric chromosomes was observed [XX, 56, rob (1;29)]. Results from the Giemsa’s stained analyses confirm that the two autosomes (2n = 57) and four autosomes (2n = 56) involved in the translocation are the bovine homologues 1 and 29.
  Krit Pinthong , Arunrat Chaveerach , Tawatchai Tanee , Runglawan Sudmoon and Piya Mokkamul
  Nepenthes gracilis Korth. is a member of carnivorous plants in family Nepenthaceae. The plants have beautiful and economically important pitchers. It is interesting to study the protein(s) correlated with the pitcher. Crude proteins were extracted from leaf, leaf with developing pitcher and developed pitcher of the same plant and analyzed by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Two protein bands with molecular weights of 42.7 and 38 kDa were obtained from young leaf and leaf with developing pitcher, respectively. The 42.7 kDa protein was identified as phosphoglycerate kinase (PGK) by Liquid Chromatography Mass Spectrometry (LC-MS/MS), but the 38 kDa band is an unknown protein. Both proteins were differentially expressed in each developing stage of the pitcher, thus may be powerful candidates play role in development pathway of leaf and pitcher.
 
 
 
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