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Articles by Arturo Reyes-Ramirez
Total Records ( 2 ) for Arturo Reyes-Ramirez
  Arturo Reyes-Ramirez and Jorge E. Ibarra
  Practically all Bacillus thuringiensis strains contain a set of self-replicating, extrachromosomal DNA molecules or plasmids, which vary in number and size in the different strains. The plasmid patterns obtained from gel electrophoresis have previously been used as a tool to characterize strains, but comparison of the plasmid patterns has been limited in the number and diversity of strains analyzed. In this report, we were able to compare the plasmid patterns of 83 type strains (out of 84) and 47 additional strains from six serotypes. The information obtained from this comparison showed the importance of this tool as a strain characterization procedure and indicates the complexity and uniqueness of this feature. For example, with one exception, all type strains showed a unique plasmid pattern. All were unique in such a way that none showed even a single comigrating plasmid in the agarose gels, and therefore, cluster analysis was impossible, indicating that plasmid patterns are qualitative rather than quantitative features. Furthermore, comparison between strains belonging to the same serotype showed a great difference in variability. Some serotypes (e.g., israelensis) showed the same basic pattern among all its strains, while other serotypes (e.g., morrisoni) showed a great diversity of patterns. These results indicate that plasmid patterns are valuable tools to discriminate strains below the serotype level.
  Beatriz A. Rodas-Junco , Hector F. Magana-Sevilla , Jose M. Tun-Suarez and Arturo Reyes-Ramirez
  The isolation of Bacillus strains with antifungal activity is important to carry out tests to protect economically important crops. We determined the in vitro activity of 13 native strains of Bacillus sp. isolated from soil against Macrophomina pasheolina using a dual assay in nutrient agar. All the strains showed inhibition of radial growth of M. phaseolina from 31-80%, with strains LUM B01 and B04 in a 80.8 and 75.9%, respectively with the highest antifungal activity. The crude extract of the strain of chitinases LUMB0 04 inhibited the growth of M. phaseolina by 30%. The strains of chitinolytic Bacillus sp. isolated from soils showed antifungal activity and have the potential to be used in biological control of M. phaseolina.
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