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Articles by Arnim
Total Records ( 5 ) for Arnim
  Yurnalis , Arnim and Dino Eka Putra
  Background and Objective: The insulin-like growth factor 1 gene (IGF1) is a candidate gene for marker-assisted selection strategies. Two single nucleotide polymorphism of IGF1 gene namely IGF1/SnaBI and IGF1/TasI has been reported to be associated with production traits in several cattle breeds. One single nucleotide polymorphism in exon 4 namely IGF1/Rsa1 has a significant influence (p<0.01) on birth weight, weaning weight and average daily gain of Bali cattle. The objective of this study was to determine the IGF1/SnaBI, IGF1/TasI and IGF1/RsaI polymorphisms and their associations with average daily gain in Pesisir cattle local breed from west Sumatera Indonesia. Methodology: The isolation of DNA from 183 blood sample was performed using DNA extraction kit (Promega DNA purification kit) following the manufacture procedure. The amplification of IGF1 fragment was done by master mix from Thermo Scientific. Results: In the case of the IGF1/SnaBI polymorphism, three genotypes were observed, frequencies were 0.011, 0.011 and 0.978 for AA, AB and BB, respectively. This gives frequencies of 0.064 and 0.9836 for A and B alleles. For IGF1-TasI locus showed that AA genotype occurred with a frequency of 0.978, AC with 0.011 and CC with 0.011 with frequency for allele A and C were 0.9836 and 0.0164, respectively. In the case IGF1/RsaI polymorphism produces only TT and CT genotype with frequency 0.027 and 0.973 respectively and frequency of T and C allele were 0.5137 and 0.4863, respectively. Conclusion: Among these 3 polymorphism only IGF1/RsaI was associated with average daily gain, Thus this polymorphism could be a potential for improving body weight in Pesisir cattle. Association study for IGF1/SnaBI and IGF1/TasI were strongly limited by low frequency of one allele.
  Arnim , Ferawati and Yetti Marlida
  The aim of this research was to test the effectiveness of coconut shell liquid smoke in preservation of meatballs. The research had been performed using factorial Completely Randomized Design (CRD) with two factors i.e. liquid smoke concentration (0%, 3%, 5% and 7%) and shelf life at refrigeration temperature (0, 5, 10 and 15 days). Parameter analyzed were water content, protein content, fat content, pH and Total Plate Count (TPC). Liquid smoke concentration had significant effect on water content, protein content, fat content, pH and Total Plate Count. Shelf life had significant effect on water content, protein content, fat content and Total Plate Count but not for pH. The interaction of liquid smoke concentration and shelf life had significant effect on protein content only. Applications of 7% liquid smoke in meatballs at 4±1°C increased shelf life until 15 days storage were better accepted and retarded the decreased of pH and moisture content compared to control. The result indicated that liquid smoke was an effective preservative agent for meatballs.
  R. Herawaty , N. Jamarun , M. Zain , Arnim and R.W.S. Ningrat
  The aim of this experiment was to study the effect of adding S. cerevisiae and leaf of L. leucocephala in diet rice straw-based of nutrients digestibility and body weight gain of cattle. The experiment was conducted in Ruminant Nutrition Laboratory of the Faculty of Animal Science, Andalas University, Padang. Experimental design used is a Latin Square Design (LSD) with four treatments and four periods. This experiment used 175.±10.53 kg male ongole crossbreed. The treatments were (A) grass+concentrates (B) rice straw+concentrates (C) was the treatment of B plus 0.5% S. cerevisiae and (D) was the treatment C+15% L. leucocephala. The results showed that the dry matter digestibility of treatment B (61.03%) were significantly lower than treatments A, C and D respectively (68.05, 63.01 and 68.15%) and supplementation of S. cerevisiae was able to improve nutrient digestibility and body weight gain of cattle but still low as compared to control (A). Addition of L. leucocephala in treatment D (850.7 g/day) was able to provide digestibility and body weight gain similar to the control ration (775.7 g/day). It can be concluded that the use of rice straw as a substitute for grass would give the same results with the grass when added 0.5% S. cerevisiae and 15% L. leucocephala.
  Nurjama yah , Yetti Marlida , Arnim and Yuherman
  Rimbo Panti hot springs located bordering the province of West Sumatra and North Sumatra, precisely in the District of East Pasaman Pasaman regency of West Sumatra Province approximately 200km from the city of Padang. Hot water samples obtained from 5 pools which have a normal temperature of 50-95°C. The aim of this research was to isolate and identify Lactic Acid Bacteria (LAB) thermophilic activity from hot spring with pathogen bacteria (Escherichia coli O157: H7, Salmonella thypmurium and Listeria monocytogenes) followed identified by PCR. The bacteria isolated were growth on medium thermus cair and then deMan Rogosa and Sharpe (MRS) Agar supplement with CaCO3 1% and then performed purification by plate out on deMan Rogosa and Sharpe (MRS) Agar. It was found 23 isolates of LAB showed with clear zone around the culture and 5 isolates (N2, N4, N6, N9 and N12) has been antimicrobial activity against the growth of pathogenic bacteria. The results showed that isolates N6 had the highest antimicrobial activity against all bacteria test, with a range of inhibition zone 18-30 mm, gram positive, spore former coccus, non motility and catalase negative. LAB isolates that have the widest diameter of the clear zone continued to test the minimum inhibitory concentration (MIC). MIC values of isolates N6 supernatant against pathogenic bacteria Escherichia coli O157: H7 by 60 and 80% of the bacterial pathogen Salmonella thypmurium and 50% of the bacterial pathogen Listeria monocytogenes. Based on morphological examination and PCR analysis, the isolate N6 was primarily identified as Pediococcus pentosaceus strain A24 bacteria.
  Afriani , Arnim , Yetti Marlida and Yuherman
  Background and Objective: Proteases are important enzymes and have high economic value due to their wide applications in the food industry as a meat tenderizer. Protease use in the food industry necessitates an understanding of the capabilities and influencing factors of these enzymes to accelerate enzymatic reactions. This study aimed to isolate and characterize the proteases of lactic acid bacteria (LAB) from Bekasam. Methodology: The samples were obtained from the third, fifth, seventh, ninth and eleventh day of fermentation to isolate the proteolytic LAB. Characterization of proteases includes the incubation time, casein substrate concentration, optimum temperature and pH, metal ion contents and stability. The LAB with the highest protease activity is identified molecularly and isolated through 16S rDNA sequencing and phylogenetic analysis based on the Neighbor Joining method. Results: The results showed that the best isolate was BAF-715 because it had the highest protease activity (18.84 U mL–1) at 40 h of incubation. The optimum activity of this protease on a casein substrate at 2.5% occurred at an incubation temperature of 40°C at pH 7 and in the presence of Mg2+ and Mn2+ (5 mM) as activators. Based on molecular DNA identification, the BAF-715 isolate is determined to be Pediococcus pentosaceus. Conclusion: A protease produced by Pediococcus pentosaceus showed the highest proteolytic activity, making it the best protease for application as a beef tenderizer.
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