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Articles by Aris Tri Wahyudi
Total Records ( 5 ) for Aris Tri Wahyudi
  Edi Husen , Aris Tri Wahyudi , Antonius Suwanto and Giyanto
  Problem statement: 1-Aminocyclopropane-1-Carboxylate (ACC) deaminase-producing bacteria have been known to promote plant growth by decreasing ethylene inhibition of various plant processes. However, their efficacy under field soil conditions may vary depending on the range and variability of the environmental factors. This study examined the ability of eight promising isolates of ACC deaminase-producing Pseudomonas to enhance soybean growth under acidic and low fertility status of field soil conditions. Approach: The bacteria were formulated into peat-based carrier and used to inoculate soybean seeds. Cell viability in the carrier was evaluated periodically. The number of bacterial population at the time of seed inoculation was above 107 cell g-1. Treated and untreated seeds were grown in plots (5x4 m2) and set in a randomized complete block design with 3 replicates. Observations were made at 30 d after planting for shoot height and weight, number of nodules and at harvesting for number of pods and yield. Results: Three out of eight isolates significantly increased soybean growth exhibited by higher number of nodules and pod filling and higher seed dry weight than those of untreated control. Those five remaining bacteria, on the contrary, inhibited soybean growth indicating that other unknown external factors influenced or covered the beneficial trait of ACC deaminase. Conclusion: Bacteria having ACC deaminase activities could be truly plant growth promoting bacteria providing that their beneficial effects are consistent at a wide range of environmental conditions.
  Rika Fithri N. Buana , Aris Tri Wahyudi and Nurita Toruan-Mathius
  Basal Stem Rot (BSR) disease caused by G. boninense is one of the most serious diseases in oil palm. Many attempts have been done to prevent or reduce infection of this disease but they have not provided optimum results. Burkholderia sp. isolated from rhizosphere and root tissue of symptomless oil palm showed potentials in suppressing G. boninense growth in vitro. Five isolates of rhizosphere Burkholderia sp. and one endophyte Burkholderia sp. were used in antagonist test against G. boninense growth on PDA media. Antifungal biosynthesis related gene from Burkholderia sp. was confirmed by using PCR with pyrrolnitrin (prn), pyoluteorin (plt), phenazine (phz) and DAPG (phl) primers. Burkholderia sp. with the highest antagonist activity were applied to oil palm germinated seed for in vivo test. Endophyte Burkholderia B212 showed the highest antagonist activity against G. boninense growth in vitro with PIRG 34.38%. The B212 genome was yield an expected PCR product by using prn primers (790 bp). Sequence BLAST result showed the gene was 99% identical with B. cepacia partial prnD gene, strain ESR63. In vivo test of B212 showed that treatment of Burkholderia B212 on plant without G. boninense infection increased the height and biomass of the plant. However, B212 did not decrease the disease incidence and disease severity on G. boninense infected plant. In addition it decreased the plant height and biomass compare to control plant.
  Rivia Kumala Dewi , Suranto , Ari Susilowati and Aris Tri Wahyudi
  Biocontrol using antagonist agent is one of environment-friendly method of controlling bacterial leaf blight disease in rice field. Eight antagonistic bacteria against Xanthomonas oryzae pathovar oryzae as well as the causal bacterial leaf blight disease in rice, have been isolated from rice phyllosphere of Wonogiri and Sukoharjo Regency, Central Java, Indonesia, using dual plate method. The aims of this study were to identify molecularly of 16S rDNA and polyketide synthase (PKS) genes of antagonist bacteria. The PKS gene is recorded as one of antibiotic compounds class which encode the polyketide biosynthesis. The amplification of 16S rDNA gene was performed using 63f and 1387r primers, while PKS gene detection was performed using degKS2F.gc and degKSR5.gc primers. The nucleotide sequences of 16S rDNA and PKS genes was aligned using GenBank database and BLAST-N program from NCBI site was operated. The results showed that the eight isolates identity of SH2a, MO142, MO22g, MO34h, MO34i, MO34j, MO43a and MO63j were Pseudomonadaceae SH2a, Pantoea sp. MO142, Pantoea sp. MO22g, Erwinia sp. MO34h, Pantoea sp. MO34i, Pantoea sp. MO34j, Pantoea sp. MO43a and Pantoea sp. MO63j, respectively. Bacterial antagonists of PKS genes have similarities with the gene of nonribosomal peptide synthetase-polyketide synthase hybrid (cpbI) Lysobacter lactamgenus. This indicates that the antagonist mechanism of antagonist bacteria is antibiosis.
  Noor Andryan Ilsan , Abdjad Asih Nawangsih and Aris Tri Wahyudi
  Bacterial Leaf Blight (BLB) caused by Xanthomonas oryzae pv. oryzae (Xoo) is the most destructive and serious disease causing productivity loss, especially in rice, in Asia. The chemical pesticide usage over a long period of time brings about adverse toxic effect on the potential production of the land and the main consumers of the products. Microbes as biocontrol agents have efficiency and safety for humans and other non-target organisms. They leave small amount or no residue in foods. Soil microbes cannot be used directly as biopesticides because common root-colonizers fail to establish on leaves. Actinomycetes are Gram positive bacteria that are known to produce bioactive compound up to 70% of the total compound produced by bacteria, including antimicrobes. Several strains of actinomycetes are known capable of protecting plants against plant disease. The aims of this study were to isolate and screen non-pathogenic phyllosphere actinomycetes of rice which are capable of controlling BLB disease in rice. A total eight isolates are positively capable of controlling Xoo in vitro and four isolates significantly reduce disease severity of BLB. Pellet of STG 15, which is the best, showed 25.87% control efficiency of BLB severity at 14 Days After Inoculation (DAI) using Xoo compared to control. Molecular identification based on 16S rRNA gene performed for six isolates with the highest activity showed that they belongs to genus Streptomyces, Actinomadura, Nonomuraea, Micromonospora. Isolate STG 15 which has the highest capability to control BLB incidenceis identified as Nonomuraea sp.
  Muhammad Asril , Nisa Rachmania Mubarik and Aris Tri Wahyudi
  Chitin is a major component of fungi cell wall, mycelia, stalks and spore which can be hydrolyzed by chitinase. This study was conducted to measure the ability of chitinase producing bacteria in degrading chitin of fungal pathogens such as Curvularia affinis and Colletotrichum gloeosporioides. These pathogens caused antrachnose, leaf blight and rotting on oil palm leaves. Chitinase producing bacteria, Bacillus thuringiensis SAHA 12.08 isolate were used in this study. SAHA 12.08 showed maximum chitinase with specific activity (7.896 U mg-1 protein) at 60 h incubation. Maximum temperature and pH of chitinase activity were 35°C and 7.0, respectively. Chitinase was partially purified by 30% ammonium sulphate precipitation could increase 2.35 fold than the specific activity. The activity of partially purified chitinase was optimal at 45°C and 7.0, respectively. This chitinase was stable at optimum temperature for 180 min incubation. On SDS-PAGE analysis, the enzyme had molecular weight of 107, 102, 82, 63, 55, 46 and 44 kDa from zymogram analysis only 82 kDa protein band showed chitinase activity. In vitro and detached leaf bioassay showed that chitinase of SAHA 12.08 had antagonist activity and biocontrol efficacy to C. affinis and C. gloeosporioides in oil palm leaves.
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