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Articles by Arif Jan
Total Records ( 5 ) for Arif Jan
  Phalisteen Sultan , A.S. Shawl , P.W. Ramteke , Arif Jan , Nahida Chisti , Neelofar Jabeen and S. Shabir
  A protocol has been developed for in vitro shoot proliferation from callus cultures of Podophyllum hexandrum Royle. Callus initiation occurs from root segments of established in vitro grown seedlings on Gamborg`s B5 media (half strength) supplemented with 2,4-D (0.5-1.5 mg L-1) and BA (0.2-1.0 mg L-1). The rhizome of this plant contains several important lignans and most important being podophyllotoxin, main precursor for anticancer drugs teniposide and ertoposide. In addition Podophyllum hexandrum has been reported to have radioprotection properties. Ruthless collection has led to the disappearance of this important medicinal plant from many areas of Himalayas. Thus there is need for immediate conservation of this important medicinal plant through tissue culture means. In the present study shoot proliferation occurs from callus cultures cultured on basal MS medium fortified with BA and IAA either alone or in combination (0.5-5.0 mg L-1) each. Regenerated shoots were rooted with high efficiency on MS medium fortified with activated charcoal (0.5-1.0%) and NAA (0.5-2.0 mg L-1). The rooted plantlets were transferred to green house in jiffy pots containing sand, soil and vermiculite in 1:1:1 ratio.
  Neelofar Jabeen , A.S. Shawl , G.H. Dar , Arif Jan and Phalisteen Sultan
  A protocol has been developed for in vitro shoot proliferation from callus cultures of Aconitum heterophyllum Wall. Callus initiation occurs from nodal segments on MS media fortified with NAA (0.5 mg L-1) and BAP (0.25 mg L-1). Callus was transferred on Msmediasupplemented with BAP (0.25 mg L-1) for shoot proliferation. The best response for shoot proliferation was obtained on MS media+ NAA (0.25 mg L-1) + BAP (0.5 mg L-1). The well-developed micro shoots were transferred to root induction media containing MS basalmedia + IAA (1.0 mg L-1). The rooted plantlets were finally transferred to green house for hardening and field transfer.
  Kousar Makeen , Garard Abrahim , Arif Jan and Archana K. Singh
  Twenty diverse mungbean (Vigna radiata L. Wilkeezek) genotypes were evaluated for the estimation of genetic variability, heritability, genetic advance, correlation coefficient and path coefficient analysis for ten quantitative characters. The genotypes differed significantly for all characters studied. Higher genotypic and phenotypic coefficient of variation was observed for seed yield and number of pods per plant. Maximum heritability values were recorded in seed protein content, plant height and test weight. High heritability coupled with high genetic advance was observed in pods per plant, plant height and test weight indicating the importance of additive gene effect for expression of these characters. Character association indicated that pods per plant and plant height have significant positive correlation with seed yield. Maximum direct effect on seed yield was observed through pods per plant, test weight and plant height.
  Sajad Mohi-ud din , F.A. Zaki , Arif Jan and F.A. Bhat
  The three bio-control agents, viz. Beauveria bassiana, B. brongniartii and Metarhizuim anisopliae proved pathogenic at each level of concentration 1x108, 1x106, 1x104 and 1x102 spore mL-1) studied, but mortality in a shorter duration was observed at a spore concentration of 1x108 spore mL-1. Enhanced mortality was observed with increase in inoculum dose.
  M.I. Shah , Phalisteen Sultan , A. Nasier , P. Williams , Arif Jan , M. Sajad , Suriya Rehman and A.S. Shawl
  The present study was conducted to see the effect of some fungicides viz., Carbendazim, Mancozeb, Sulphur and conjoint Carbendazim and Mancozeb against Fusarium oxysporum in the division of Microbiology and Mycology of this laboratory. Poisoned food technique was adopted for carrying out this experiment for measuring radial growth of the test fungus in cm. All the treatments significantly reduced the growth of the F. oxysporum udum as compared to control but it was observed that the growth of the fungus were significantly less in 10,000 ppm concentration as compared to 10, 100 and 1000 ppm concentration of fungicides. On comparative analysis of different fungicides tested Mancozeb showed maximum inhibition of F. oxysporum, udum as compared to other fungicides.
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