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Articles by Arbakariya Ariff
Total Records ( 2 ) for Arbakariya Ariff
  Suat Hian Tan , Radzali Musa , Arbakariya Ariff and Mahmood Maziah
  Problem statement: Considering pegaga medicinal properties and over-exploitation, the requirement for a tissue culture technique as an alternative production system was crucial. Approach: Investigation of cell suspension culture response to different plant growth regulators (PRGs) for flavonoid production from elite cell line was carried out. Callus cultures were initiated from the leaf explants of Centella asiatica on Murashige and Skoog (MS) medium containing B5 vitamins and 30 g L-1 sucrose supplemented with different concentrations (0.5-2.5 mg L-1) of 2,4-D, NAA, Dicamba, Picloram and IBA supplied singly and in combination with different concentrations (0.5-1.5 mg L-1) of kinetin, BAP and TDZ. Results: Callus induction was observed for all the PGRs tested. The highest callus induction frequency (86.67%) was observed in MS medium containing 2.0 mg L-1 2,4-D while the combination of 2.0 mg L-1 2,4-D and 1 mg L-1 kinetin in MS medium gave the highest biomass yield (0.27 g dry weight culture-1). This combination was also found to be best for callus proliferation for all the accessions investigated. Among the four accessions tested, UPM03 was found to have the highest biomass yield (0.041 g DW culture-1) and hydrolysed flavonoid content (10.75 mg g-1 DW) after the 12th day of culture. The flavonoids present in the four accessions were quercetin, kaempherol, luteolin and rutin based on High Performance Liquid Chromatography (HPLC) analysis. These results indicated that C. asiatica accession UPM03 was the potential elite cell line in mass production of flavonoid, especially luteolin. Coclusions/Recommendations: In the establishment of cell suspension culture, 2 mg L-1 2,4-D and 1 mg L-1 kinetin were the best PGRs in supporting the cell growth and flavonoid production. This is the first report on the use of PRGs on the establishment of cell suspension cultures in flavonoid production of C. asiatica.
  Mahdi Shahriarinour , Mohd Noor Abd Wahab , Arbakariya Ariff and Rosfarizan Mohamad
  Screening and isolation of cellulolytic fungi was done using compost of oil palm empty fruit bunch conducted at a local factory located in Sri Ulu Langat, Dengkil, Selangor, Malaysia. This research was aimed to isolate of cellulolytic fungi with over production of cellulase components. Ten isolated fungi had shown the ability to degrade cellulose base on decolorization of CMC selective agar using Grams iodine as color indicator and cellulase production in shake flask fermentation. Aspergillus (R4) was selected as over producer of cellulase enzyme among ten isolated fungi. The Aspergillus (R4) with highest clearing zone on CMC agar (50 mm) and cellulolytic activity (CMCase 3.05, FPase 0.61 and β-glucosidase 1.75 U mL-1) was identified as Aspergillus terrus. We identified Aspergillus terrues as highly cellulolytic in producing cellulase activity. Identification of over producers of cellulase enzymes can help industries exploits of this enzyme.
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