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Articles by Anusorn Cherdthong
Total Records ( 5 ) for Anusorn Cherdthong
  Sineenart Polyorach , Metha Wanapat , Onanong Poungchompu , Anusorn Cherdthong , Pongsatorn Gunun , Nirawan Gunun and Sungchhang Kang
  Background and Objective: Cassava (Manihot esculenta Crantz) is widely grown in sub-tropical and tropical areas, producing roots as an energy source containing high soluble carbohydrate but low in crude protein. The process of protein enrichment of animal feed using microorganisms in a semi-solid culture to improve the nutritional value of ruminants feed has been considered. This study aimed to investigate the effect of microorganism fermentation on nutritional values of cassava products and in vitro rumen fermentation and digestibility. Materials and Methods: The experimental design was a 2×4 factorial arrangement in a completely randomized design. Factor A was two types of cassava root (fresh cassava root (FC) and cassava chip (CC)) and factor B was four sources of microorganism inclusion [no microorganism (No), Yeast (Y), effective microorganism (EM) and Yeast+EM (EMY)), respectively. Results: The results found that crude protein of cassava root was dramatically increased by Y and EM fermentation and the highest was found in CC (p<0.05). The gas kinetics, cumulative gas production (96 h) and in vitro dry matter and organic matter digestibility were enhanced by Y and EM fermentation (p<0.05), especially in CC group. Moreover, Y and EM could increase concentration of volatile fatty acids and ammonia-nitrogen while reduced methane production (p<0.05). Ruminal bacteria and fungi were increased whereas protozoa population was reduced by Y and EM fermentation. Conclusion: In conclusion, Y and EM fermentation could improve nutritional values of cassava products and enhance nutritional digestibility, rumen fermentation efficiency while decrease protozoa and methane production. However, further researches in feeding trial could be conducted.
  Anusorn Cherdthong , Metha Wanapat , Phongthorn Kongmun , Ruangyote Pilajun and Pichad Khejornsart
  Four rumen-fistulated, male swamp buffalo were randomly assigned according to a 4x4 Latin square design to evaluate the effect of the urea-treated rice straw to concentrate ratio (R:C) on rumen fermentation, nutrient digestibilities, microbial protein synthesis and cellulolytic bacterial population. Animals were fed R:C of 100:0, 75:25, 50:50 and 25:75, respectively. Results showed that digestibility of nutrients were significantly affected by R:C especially those of OM and fiber. However, digestibility of CP, ruminal NH3-N and plasma urea N were similar among treatments (p>0.05) whereas ruminal pH was decreased significantly (p<0.01) when concentrate ratio was increased. Total VFA concentrations and C3 were significantly different among treatments and were greatest at 50:50 of R:C supplementation (p<0.01). Total viable bacteria, proteolytic bacteria and bacteria cell count were not altered among treatments (p>0.05) whereas amylolytic bacteria, cellulolytic bacteria and fungal zoospore were significantly different (p<0.01), responding to a change in proportion of R:C. Moreover, using of real-time PCR technique provided that feeding of a 100% roughage remarkably increased these three cellulolytic bacteria numbers up to 3.54x109 copies mL-1 for F. succinogenes, 7.38x107 copies mL-1 for R. Flavefaciens and 5.80x106 copies mL-1 for R. albus in rumen digesta, respectively. It is most notable that F. succinogenes were the highest in population in the rumen of swamp buffalo. In addition, efficiency of rumen microbial N synthesis were enriched by R:C supplementation, especially at the ratio of 50:50 (p<0.05). Based on this study, it could be concluded that supplementation of R:C at 50:50 improved digestibilities of nutrients, ruminal ecology and microbial protein synthesis efficiency.
  Sungchhang Kang , Metha Wanapat , Parwadee Pakdee and Anusorn Cherdthong
  Four Thai-rumen fistulated swamp buffaloes male (Bubalus bubalis), about 3 years old with 360±18 kg liveweight were assigned according to a 2x2 factorial arrangement in a 4x4 Latin square design to receive dietary treatments. The treatments were as follows: T1) level of concentrate at 0.1% BW with Leucaena leucocephala Leaf Meal (LLLM) at 300 g/hd/day; T2) concentrate at 0.2% BW with LLLM at 300 g/hd/day; T3) concentrate at 0.1% BW with heated Leucaena leucocephala Leaf Meal (HLLLM) at 300 g/hd/day and T4) concentrate at 0.2% BW with HLLLM at 300 g/hd/day. The results revealed a significant increase in roughage and total DM intake (p<0.05) by concentrate level at 0.2% BW (T2 and T4) as compared with concentrate level at 0.1% BW (T1 and T3). Digestion coefficient (%) of DM, OM and CP were increased by level of concentrate at 0.2% BW while NDF and ADF were similar among treatments. However, there was no effect of neither energy level nor HLLLM on ruminal pH and temperature (p>0.05). Concentration of ruminal NH3-N was decreased by HLLLM as compared with LLLM (p<0.05) while blood urea-nitrogen was not changed and was in normal range. Total bacterial direct counts were found significantly different (p<0.05) whereas fungi zoospores and protozoal populations were similar among treatments. Nevertheless, viable bacterial counts were found affected by both concentrate level and HLLLM. The treatments with HLLLM were lower than those in LLLM and concentrate level at 0.2% BW were higher than those supplemented at 0.1% (p<0.05). Based on this study, it could be concluded that HLLLM could be used as a protein source in terms of rumen undegradable protein while the combination of HLLLM and concentrate level at 0.2% of BW could enhance the voluntary feed intake, nutrient digestibility, rumen fermentation and ecology in swamp buffalo fed supplementation on 2+2% urea-lime treated rice straw.
  Sarunyu Chuelong , Theerawat Siriuthane , Kitsada Polsit , Sommas Ittharat , Uthai Koatdoke , Anusorn Cherdthong and Sittisak Khampa
  Nine, two-years old of crossbred native cattle were used to examine the effects of supplementation levels of palm oil in yeast culture fermented cassava pulp on rumen fermentation and average daily gain. The cows were randomly allocated in a complete randomized design and three replicates (animals) per treatment. The first group (control) was fed on a yeast fermented cassava pulp without palm oil (YFCP0), The second group was fed yeast fermented cassava pulp + palm oil at 1% (YFCP1)and third groups was fed yeast fermented cassava pulp + palm oil at 2% (YFCP2), respectively. The cows were offered the treatment diets at 2%BW and rice straw was fed ad libitum. The results have revealed that supplementation of dietary treatment on feed intake, ruminal pH, ammonia-nitrogen and blood urea nitrogen concentration were non-significantly different, while Average Daily Gain (ADG) and cost production were significantly different and had highest in cattle receiving YFCP2 than those fed YFCP1, YFCP0 diets (633.1, 614.5 and 511.1 g/day of ADG and 0.92, 0.81, 0.73 US$/kgBW of cost production, respectively). The populations of bacteria and fungal zoospores were significantly different as affected by levels of palm oil supplementation. Especially, supplementation of YFCP2 in cattle had highest increase populations of bacteria and fungi zoospore than those fed YFCP1 and YFCP0 but decreased protozoal populations. Therefore, supplementation levels of palm oil at 2% in Yeast Culture Fermented Cassava Pulp (YFCP1) as supplement diets with rice straw as roughage source could highest improved ruminal fermentation efficiency, average daily gain including increase populations of bacteria and fungi zoospores, but decreased protozoal populations in rumen of crossbred native cattle.
  Kitsada Polsit , Sarunyu Chuelong , Teerawat Siriuthane , Sommas Ittarat , Uthai Koatedoke , Anusorn Cherdthong and Sittisak Khampa
  Ten, two-years old of crossbred native cattle weighing about at 250±20 kg were randomly divided into two groups according to receive two groups of supplemental dietary treatments by receiving YFCP1 + YFCP2 (T1) and YFCRR + YFDH (T2). The cows were offered the treatment diets at 2%BW and rice straw was fed ad libitum. Means were compared using pair t-test. All animals were kept in individual pens and received free access to water. The results have revealed that supplementation of dietary treatment on feed intake was non-significantly different, while average daily gain (ADG) and rumen microorganisms especially bacteria and fungi zoospores were significant different and cattle in heifer fed YFCRR + YFDH (T2) treatments and received YFCP1 + YFCP2 (T1) (646.4 and 533.2 g/d). In addition, the ruminal pH, ammonia-nitrogen and blood urea nitrogen concentration were non-significantly different. Supplementation of T2 could improve population of bacteria and fungal zoospore higher than those fed T1, but decreased populations of Holotrich and Entodiniomorph protozoa in rumen. The results indicate that supplementation of yeast fermented cassava and durian hull as supplement diets with rice straw as roughage source could improve ruminal fermentation efficiency, average daily gain in crossbred native cattle.
 
 
 
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