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Articles by Antonius Suwanto
Total Records ( 2 ) for Antonius Suwanto
  Edi Husen , Aris Tri Wahyudi , Antonius Suwanto and Giyanto
  Problem statement: 1-Aminocyclopropane-1-Carboxylate (ACC) deaminase-producing bacteria have been known to promote plant growth by decreasing ethylene inhibition of various plant processes. However, their efficacy under field soil conditions may vary depending on the range and variability of the environmental factors. This study examined the ability of eight promising isolates of ACC deaminase-producing Pseudomonas to enhance soybean growth under acidic and low fertility status of field soil conditions. Approach: The bacteria were formulated into peat-based carrier and used to inoculate soybean seeds. Cell viability in the carrier was evaluated periodically. The number of bacterial population at the time of seed inoculation was above 107 cell g-1. Treated and untreated seeds were grown in plots (5x4 m2) and set in a randomized complete block design with 3 replicates. Observations were made at 30 d after planting for shoot height and weight, number of nodules and at harvesting for number of pods and yield. Results: Three out of eight isolates significantly increased soybean growth exhibited by higher number of nodules and pod filling and higher seed dry weight than those of untreated control. Those five remaining bacteria, on the contrary, inhibited soybean growth indicating that other unknown external factors influenced or covered the beneficial trait of ACC deaminase. Conclusion: Bacteria having ACC deaminase activities could be truly plant growth promoting bacteria providing that their beneficial effects are consistent at a wide range of environmental conditions.
  Susan Soka , Antonius Suwanto , Dondin Sajuthi and Iman Rusmana
  Tempeh is a popular Indonesian fermented food made from soybean that can be a good source of dietary fibers for human health. Dietary fibers have the ability to modulate gut microbiota composition in order to improve human health. In this study, Sprague-Dawley (SD) rats were fed a standard diet supplemented with cooked and de-hulled soybean or tempeh for 28 days. The specific bacterial groups in fecal samples were quantified using real-time polymerase chain reaction with 16S rRNA gene-targeted, group-specific primers. Populations of Bacteroidetes, Bacteroides fragilis, Firmicutes and Clostridium leptum increased when supplemented with tempeh. In addition, the ratio of Firmicutes/Bacteroidetes was lower in groups fed with either raw or cooked tempeh compared to the soybean group. Previous studies showed that obese hosts have higher ratios of Firmicutes/Bacteroidetes compared to the lean hosts. Increased Bacteroidetes populations after tempeh supplementation indicated that tempeh might modulate the composition of gut microbiota toward a healthier gut.
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