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Articles by Anne Nuraini
Total Records ( 4 ) for Anne Nuraini
  Dikayani , Anas , Anne Nuraini and Warid Ali Qosim
  Background and Objective: Banana plants are agricultural crops, particularly horticulture, mostly consumed by many people. Banana plants are considerably influenced by biotic and abiotic environmental factors. Stresses caused by abiotic factors, is one of which caused by salinity, will result in decreased production of bananas, because these plants are very sensitive to salinity. This study aimed to investigate the response of banana plants to salinity stresses in vitro culture. Materials and Methods: Explants of banana Musa acuminate L. var. Barangan and basic medium murashige and skoog, benzyl amino purine, sugar, jelly, NaOH, HCl were used. Statistic analyzed with analysis of variance and Duncan test. The experimental design used was randomize complete design with two factors, factor I: Sodium chloride (NaCl) treatments of various concentrations, i.e., 0, 50, 100, 150 and 200 mM and factor II: Time after culture, in 1, 2, 3 and 4 weeks after culture. Observations over growth parameters were performed on height, wet weight and dry weight of banana plantlets at 1, 2, 3 and 4 weeks after cultures. Statistical analysis was done by one way analysis of variance. Results: The statistical analysis showed no significant differences in the plant height, salinity inhibits the growth of banana plantlets in term of the parameters of height, wet weight of shoots and roots, dry weight of shoots and roots of the banana plantlets. A treatment of 200 mM NaCl indicated that plantlet growth is inhibited in term of height (2.6500 cm), wet weight of shoots (0.0917 g) and roots (0.1957 g) and dry weight of shoots (0.0096 g) and roots of banana plantlets (0.02009 g). Conclusion: Plant growth in saline stressed concentration of NaCl 50, 100 and 150 mM.
  Jajang Sauman Hamdani , Anne Nuraini and Syariful Mubarok
  Background and Objective: One problem in introducing potato cultivation in medium land of Indonesia is high temperature which causes low yield of the potato tuber. High temperature improves shoot growth but reduces the formation and development of tuber. To reduce high temperature and inhibit shoot growth, therefore new strategy must be conducted to resolve this problem such as by the application shading net and paclobutrazol during potato cultivation. The objective of this study was to evaluate the interaction effect between shading net and paclobutrazol on growth and yield of potato ‘medians’ tuber of G2 in medium land of Indonesia. Methodology: Randomized block design was used in this study with two factors, consisted of three levels of shading net density (0, 30 and 40%) and 4 levels of paclobutrazol concentration (0, 50, 100 and 150 mg L–1). All parameters were tested by two-ways of analysis of variance (ANOVA) using Fisher test followed by Duncan’s multiple range test at the 5% significance level. Results: The result showed that no significant interaction between shading net density and paclobutrazol concentration at all observed parameters. Shading net 30% increased plant height, leaf area index, tuber number, tuber weight and percentage of tuber for seed size that was significantly higher than without and 45% shading net. Moreover, paclobutrazol suppressed plant height and leaf area but it increased chlorophyll content, tuber number and tuber weight/plant and percentage of tuber for seed size. Conclusion: Paclobutrazol suppressed plant height and leaf area, however it increased leaves chlorophyll content, tuber number and tuber weight/plant. The highest tuber number and percentage of class tuber for seed size were obtained from plant with 50 mg L–1 of paclobutrazol application.
  Anne Nuraini , Sumadi , Syariful Mubarok and Jajang Sauman Hamdani
  Background and Objectives: Potato production in Indonesia, at a medium altitude, can have a number of problems. Therefore, to increase potato productivity, there must be some alternative. One method is with the application of paclobutrazol. The objective of this study is to investigate the effects of application time and paclobutrazol concentration on the yield of potato seeds (G2 of cultivar medians) grown at a medium altitude. Materials and Methods: This experiment was conducted in the Research Station of Agriculture Faculty of Universitas Padjadjaran at an altitude of 685 m a.s.l. with a D3 rainfall rate and inceptisol soil. The experiment used a randomized plot design: The main plot factor was the application time of paclobutrazol at 30 DAP (days after planting) and 45 DAP, with the second factor being the concentration of paclobutrazol at 0, 50, 100 and 150 ppm. All parameters were tested by analysis of Variance (ANOVA), followed by Duncan's multiple range test at the 5% significance level. Results: Results showed there was no interaction between application time and paclobutrazol application on either the growth or yield of G2 potato seeds. However, application of paclobutrazolat45 DAP showed taller plants, with more and heavier tubers compared to application at 30 DAP. Paclobutrazol application reduced the plants’ height, leaf area and dry weight of the plants. On the other hand, it increased chlorophyll content index, numbers of tubers and weight per plant. The use of50 ppm paclobutrazol resulted in the highest number of potato seeds of cultivar medians at 92.3%. Conclusion: In summary, the application of paclobutrazol at 50 ppm and 45 DAP is an optimal application for increased plant productivity of potatoes at medium altitude.
  Dikayani , Anas , Anne Nuraini and Warid Ali Qosim
  Background and Objective: Quality banana fruit is significantly related with number and type of metabolite produce. Metabolite production of Musa acuminata L. is effected by abiotic factors such as salinity. So the purpose of this research was to study the effect of plant response under salinity stress and also identified the chemical compound especially phenolic compound. Materials and Methods: Explants used in this research were shoot of Musa acuminata L., Murashige and Skoog medium, Benzyl amino purine, sugar, agar, sodium chloride. The research was conducted in two stages; stage (1): Developing in vitro culture of shoot of Musa acuminata L. with NaCl treatment i.e., 0, 50, 100, 150 and 200 mM concentration, stage (2): Gas Chromatography Mass Spectrometry (GCMS) analysis on the shoot of in vitro banana plantlets. Results: The findings of this study showed that compound content of shoot of banana plantlets were Amine, Ester, Propane, Keton, Alkohol, Phenol, Methyl, Acid and it was observed that Ester (27.515%) was the highest detected compound. Conclusion: It is concluded that the salinity had affected the plant growth. The analysis showed that the highest compound content of Musa acuminate L. Barangan cultivar was Ester and Phenols.
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