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Articles by Anna Ling Pick Kiong
Total Records ( 4 ) for Anna Ling Pick Kiong
  Sobri Hussein , Rusli Ibrahim , Anna Ling Pick Kiong , Nor`aini Mohd Fadzillah and Siti Khalijah Daud
  The first successful micropropagation protocol for an important recalcitrant medicinal plant in Southeast Asia, Eurycoma longifolia Jack or Tongkat Ali as known in Malaysia was developed. In attempts to establish the somatic embryos of E. longifolia, the potential of cotyledon, zygotic embryo, leaf, petiole, stem and taproot in forming embryogenic callus were examined in the basal Murashige and Skoog (MS) medium supplemented with different auxins at various concentrations. Only cotyledon explants were able to form embryogenic callus in the presence of 1.0 mg L -1 (w/v) of 2,4-dichlorophenoxyacetic (2,4-D). A higher yield (60%) of embryogenic callus was obtained when the Type 4 method dissected cotyledon explants were cultured in basal MS medium containing 0.5 mg L -1 (w/v) of kinetin and 1.0 mg L -1 (w/v) of 2,4-D. The highest number of somatic embryos (45±2) was observed in the same medium formulation with the addition of 1.0 g L -1 (w/v) activated charcoal. Subsequent transfer of these mature somatic embryos in basal MS media supplemented with 1.0 mg L -1 (w/v) of kinetin produced a 90% of plantlet regeneration. The differences between the embryogenic and non-embryogenic callus were also determined based on the histological studies.
  Anna Ling Pick Kiong , Then Chia Nee and Sobri Hussein
  Murashige and Skoog (MS) nutrient media supplemented with auxins 2,4-dichlorophenoxyacetic acid (2,4-D), Naphthaleneacetic acid (NAA), Indole-3-acetic acid (IBA) and 4-amino-3, 5, 6-trichloro picolinic acid (picloram) each at the concentrations of 1, 3 and 5 mg L-1 were evaluated for their effects on the callus induction from the leaf explants of Ficus deltoidea. The best calli with healthy morphology and growth was obtained from explants cultured on MS media supplemented with 3 mg L-1 picloram. Friable calli with white, greenish or yellowish colour were obtained after 10 days upon culturing. 2, 4-D and IBA induced morphologically unhealthy calli, whereas cultured explants gave a negative response to MS media supplemented with various levels of NAA. For callus maintenance, the study indicated that media supplemented with 1 mg L-1 picloram gave the best growth rate of calli and was the most suitable callus maintenance medium to be used.
  Sobri Hussein , Rusli Ibrahim and Anna Ling Pick Kiong
  This study reported the first successful adventitious shoots regeneration from the root and stem explants of E. longifolia. Generally, stem explants appeared to have a lower regeneration capability when treated with the cytokinins alone compared to the root explants. In the root explant, the best medium formulation determined was basal Juglans medium (DKW) supplemented with 1.0 mg L-1 of kinetin + 1.0 mg L-1 of zeatin whereas in the stem explants, basal woody plant medium (WPM) enriched with 2.0 mg L-1 of BAP + 2.0 mg L-1 of zeatin was found to be the best medium formulation in increasing the regeneration rate. Stem and root explants that were 2.0 cm distant from one another has been identified as the most suitable position for attaining the maximum percentage of adventitious shoots formation. Rootlets induction of adventitious shoots formed was also achieved and the highest induction rate was attained in basal MS medium supplemented with 0.5 mg L-1 of Indole-3-butyric acid. Acclimatization of the in vitro plantlets produced survived well with no morphological differences from the parent plants after two months of transplantation to the soil.
  Anna Ling Pick Kiong , Maziah Mahmood , Nor’aini Mohd Fadzillah and Siti Khalijah Daud
  Production of the four targeted triterpenes, asiatic acid, madecassic acid, asiaticoside and madecassoside in leaf derived callus and cell suspension cultures of Centella asiatica was compared with its production in whole plant from field and in vitro shoot cultures. In callus a cultures, production was found the highest during the third week of culture and the contents declined gradually. The glycosides, madecassoside and asiaticoside contents were found higher than the asiatic acid and madecassic acid in callus cultures. Four triterpenes precursors, squalene, Farnesyl Pyrophosphate (FPP), Isopentenyl Pyrophosphate (IPP) and leucine, which are involved directly or indirectly in the triterpene biosynthetic pathway, were used to increase the triterpenes production. Squalene was found the best precursor in promoting triterpenes production in callus cultures. Squalene treated callus also produced the highest biomass production compared to the other precursors tested.
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