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Articles by Anita Menconi
Total Records ( 3 ) for Anita Menconi
  Adriano Sakai Okamoto , Raphael Lucio Andreatti Filho , Ticiana Silva Rocha , Anita Menconi and Guilherme Augusto Marietto-Goncalves
  Pathogenic bacteria possess genes responsible for virulence, that when expressed, determine not only bacterial invasion and persistence and destruction of host cells but also survival capacity under inappropriate culture conditions. This study utilized 100 samples of Salmonella enterica subspecies enterica Serotype Enteritidis (SE) isolated from avian material, detected the virulence genes InvA and SpvC by medium of Polymerase Chain Reaction (PCR) and compared them for possible expression of virulence factors for survival in hostile conditions of temperature, pH and reduced concentration of nutrients necessary for SE multiplication. Of all the samples analyzed, two presented the genes SpvC and InvA, simultaneously, with one probable expression of them being verified in growth with pH 10.0 or temperature of 25oC. But in relation to nutrient concentration, neither sample obtained growth when seeded in medium containing 0.5% nutrient.
  Anita Menconi , Xochitl Hernandez-Velasco , Juan David Latorre , Gopala Kallapura , Neil R. Pumford , Marion J. Morgan , B.M. Hargis and G. Tellez
  Two experiments were conducted to evaluate the effect of chitosan as a biological sanitizer on chicken skin during storage. For experiment 1 (two trials) five skin samples of equal size were dipped into a solution containing 106 cfu/mL of Salmonella Typhimurium (ST) for 30s. Skin samples were then removed and dipped into a solution containing PBS or 0.5% chitosan for 30s. In experiment 2, aerobic Gram negative spoilage bacteria were used as indicators instead of ST. In both experiments, all samples were placed in individual bags and kept at 4°C. In experiment 1, dipping ST contaminated skin samples in a solution of 0.5% chitosan reduced (p<0.05) the recovery of ST by 24 h. In experiment 2, 0.5% chitosan treatment solution reduced (p<0.05) the presence of spoilage-causing psychrotrophic bacteria below detectable levels. These results suggest that 0.5% chitosan has a potential for use in an intervention technology for the control of foodborne pathogens on the surface of chicken skin contaminated with bacteria during storage.
  Claudia E. Gutierrez-Fuentes , Luis A. Zuniga-Orozco , Jose Luis Vicente , Xochitl Hernandez-Velasco , Anita Menconi , Vivek Ayamchirakkunnel Kuttappan , Gopala Kallapura , Juan David Latorre , Sherry Layton , Billy Marshall Hargis and Guillermo Tellez
  Probiotics are live microorganisms which, in adequate dose, will increase the beneficial microbial population in gut. A commercial lactic acid bacteria-based probiotic FloraMax-B11® (FM) has shown to have beneficial effect in reducing microbial colonization in broilers. The present study was intended to evaluate the effect of FM on growth performance, bone qualities and morphometric analysis of broiler chickens. In experiment 1, broiler chickens were divided into control or FM treated chickens. Treated chickens received 5 doses of FM. At the end of 30 days, body weight, was recorded and all chickens were humanely killed. Tibias and ileum content were collected. A significant (p<0.05) increase in body weight was observed in the group that received the probiotic treatment when compared with control non treated group. The improved performance was associated with a significant (p<0.05) reduction of energy and protein digested content of the distal ileum as well as bone parameters. Experiment 2 consisted of two independent trials. In each trial, 400 day-of-hatch, broiler chickens randomly assigned to probiotic or control non treated chickens. At days 1, 12, 23, 34 and 45 days of age, treated chickens received the probiotic in the drinking water. In both trials, a significant (p<0.05) improvement in body weight, feed conversion and morphometric changes in gut and tibia were observed in the group that received FM. Estimation of the cost benefit suggested a 1:24 ratio by using FM. The results of this study suggest that the increase in performance and bone parameters in neonatal chickens treated with FM probiotic may be related with improved morphometric changes in the mucosa of duodenum which are also related with improved digestibility.
 
 
 
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