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Articles by Anirudha Rishi
Total Records ( 2 ) for Anirudha Rishi
  Sonam Sneha , Anirudha Rishi and Subhash Chandra
  In this study the 21 days old seedlings of Pennisetum glaucum were subjected to short term salt stress in order to observe the effect on chlorophyll content, protein and antioxidative enzyme activity (CAT and APX) responses. The aim of the experiment was to evaluate the changes in chlorophyll content and antioxidant enzymes (Catalase and Ascorbate peroxidase). The 21 days old seedlings were subjected to salt stress by supplementing Hoagland’s solution with different concentrations of Sodium chloride (50, 100, 150 and 200 mM). Measurement of chlorophyll content and antioxidant enzyme activity were taken at different time intervals of salt treatment (12, 24, 48, 72, 96 and 120 h). The results showed statistically significant differences in traits for the salt treatment. A significant increase in Catalase activity was observed under all the salt concentrations while no significant activity of Ascorbate peroxidase activity was observed. The protein content and chlorophyll content decreased with increasing salt concentration. This result shows salt stress affects the photosynthesis rate by decreasing chlorophyll content. Catalase enzyme plays an important role in scavenging reactive oxygen species generated due to salt stress in the plant cell.
  Sonam Sneha , Anirudha Rishi , Amit Dadhich and Subhash Chandra
  Salinity is a major threat to agriculture, plants exhibits a variety of responses to salt stress that enable them to tolerate and survive in such conditions. Salinity affects physiological and biochemical processes in plants. A short term salt stress induced physiological and biochemical response were observed in P. glaucum. The experiment was conducted to understand the influence of salinity on seed germination, proline and free amino acid accumulation in P. glaucum. It was observed that as the salt concentration increased the germination percentage decreased as compared to control as well as the root/shoot length also decreased. This suggests that salinity greatly influences the germination as well as the plant growth. The levels of biochemical components proline and free amino acid were measured during the salt stressed condition. The 14 days old seedlings were subjected to 4 salt treatments (50, 100, 150 and 200 mM NaCl), free proline and free amino acids was calculated at 0, 12, 24, 48, 72 and 96th hour. Proline and free amino acid content in the salt stressed tissues increased with increase in salt concentration as well as with duration of salt stress. This result suggests that proline and free amino acid acids acts as compatible solutes in P. glaucum to protect the cellular macromolecules, maintain the osmotic balance and also scavenge the free radicals under salt stressed condition.
 
 
 
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