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Articles by Anima Sharma
Total Records ( 2 ) for Anima Sharma
  Tarun Kumar Kumawat , Anima Sharma and Seema Bhadauria
  Background: Keratinous wastes are recognized as solid waste and troublesome environmental pollutant generated from poultry farms, slaughter houses and barber’s shops and are abundant in Jaipur district, Rajasthan. Keratinous waste degradation by biological methods has been increasingly interested because of environmental awareness. Methodology: The present study was aimed at the biodegradation of keratinous wastes in an eco-friendly way by a highly efficient keratinophilic fungus namely Arthroderma multifidum (KU560574) isolated from poultry farm for the first time in Rajasthan. Results: The strain was identified by morphological traits and confirmed by the BLAST search of sequences of the ITS1-5.8S - ITS2 rDNA region. Arthroderma multifidum was inoculated into the Basal Salt Medium (BSM) with 2.5% (w/v) keratinous waste substrates to observe keratin degradation by measuring the degree of degradation and changes in pH. The rate of keratin degradation was expressed as weight loss. In this study, keratinous waste degradation was found maximum on the chicken feathers (39.2%) after 24 days and the pH increased from the initial 7.0-8.15 at 30°C by colonization of A. multifidum. The medium’s alkalinity was increased due to keratin substrate degradation at different incubation time. Maceration of keratinous waste substrates by fungi was also shown by microscopic examinations. Conclusion: This strain may be used efficiently for keratin waste management and production of industrially important protease, which would be extremely useful for biotechnological processes involving keratin hydrolysis.
  Avinash Marwal , Surendra Meena , Subhash Chandra and Anima Sharma
  Dermatophytes as the name suggest are the fungus that feed on skin. The chief source of their growth is keratin which is widely available in skin, nails and hairs. Here we have evaluated an In vitro study of antidermatophytic activity of Mint (Mentha piperita) against two dermatophytes i.e., Trichophyton rubrum and Microsporum canis. The data in the manuscript is very much helpful in curing dermatophytic infections as an application from Biotechnological point of view. Distribution of Trichophyton rubrum and Microsporum canis was found to be 19.23 and 32.69%, respectively. At variable temperature Trichophyton rubrum and Microsporum canis showed maximum growth at 37°C (0.23 and 0.19 g dry weight of mycelium, respectively). At variable pH Trichophyton rubrum showed maximum growth at pH 7.5 and 8.0 (0.32 g dry weight of mycelium) and Microsporum canis showed maximum growth at pH 7.5 (0.39 g dry weight of mycelium). Mentha piperita highest keratinase activity against Microsporum canis was found to be 2.99 unit mL-1 with 2.91 mg mL-1 extracellular release of protein and in Trichophyton rubrum it was found to be 2.99 unit mL-1 with 2.75 mg mL-1 extracellular release of protein.
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