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Articles by Anggraini Barlian
Total Records ( 5 ) for Anggraini Barlian
  Kusnandar Anggadiredja , Anggraini Barlian , Yangie Dwi Marga Pinang and Dian Anggraeny
  Nicotine is an addictive substance with detrimental effects on health. Several measures have been developed to help addicts quit smoking, yet the rate of increase in number of smokers does not seem to have slowed down. The current study was aimed to investigate the effect of Virgin Coconut Oil (VCO) on nicotine dependence and relapse using Conditioned Place Preference (CPP) paradigm in rats. The rats were randomly assigned to one of the following treatment groups: place conditioning without drug treatment before nicotine (0.5 mg kg-1, i.p.); place conditioning with oral VCO at 5 mL kg-1 or the reference drug diclofenac (3.2 mg kg-1, i.p.) 30 min prior to each nicotine treatment. The conditioning training lasted for 5 days, followed by conditioning test on the following day. In different batch of rats, the conditioning test continued with a 7-day nicotine-free period and the rats were then challenged on the following day with 0.5 mg kg-1 nicotine intraperitoneally. VCO and diclofenac were given 30 minutes before nicotine challenge to test their effects. Results showed that VCO and diclofenac significantly decreased (p<0.01) the preference to nicotine-paired compartment to those of preconditioning levels in both preference tests. Taken together, results of the present study indicate that VCO prevents nicotine dependence as well as relapse. The results further lay foundation for the development of potent agents for nicotine dependence.
  Anggraini Barlian , Kusnandar Anggadiredja , Astuti Kusumorini and Utami Ekawati
  Frog skin histology has been largely explored, particularly in relation to the skin-derived secretions, among which a number of peptides have shown potential medicinal applications. Duttaphrynus melanostictus is a frog species ubiquitous in Indonesia; however, there is only limited information with regard to skin structural characteristics as well as its potential use. The present work explored the structure of the frog skin and further assessed antifungal activity of its extract. Structural studies were carried out by Scanning Electron Microscopic (SEM) assays while antifungal evaluations were performed by testing the activity of the lyophilisate of skin extract against the pathogenic fungi Candida albicans, Microsporum gypseum and Trichophyton mentagrophytes. Results of the structural studies revealed features common to frog species, in particular an extensive distribution of secretory glands. In addition, we observed a flattened, irregular-shaped structure, believed to be secretory ‘vesicle’ of the mucous gland, considered distinct to D. melanostictus. Antifungal studies demonstrated equipotent activity of the lyophilisate against all test fungi. Taken together, results of the present work shed some light on the structural characteristic of D. melanostictus frog skin and further open an opportunity for the development of alternative antifungal agents.
  Anggraini Barlian , Kusnandar Anggadiredja and Astuti Kusumorini
  The treatment of fungal infection poses particular challenge for the medical field. Thus, the development of antifungal agents has only been progressed slowly, partly due to the considerably high toxicity of the agents. We have previously demonstrated the antifungal effect of the skin extract from Duttaphrynus melanostictus frog. In the current study, the antifungal effect was particularly investigated in the lyophilisate of granular gland secretion of the frog skin against the pathogenic fungi Candida albicans, Mycrosporum gypseum and Trychophyton mentagrophytes. Antifungal test of the lyophilisate was performed by agar diffusion method, while the study of its mechanism of action was carried out under Scanning Electron Microscope (SEM). The lyophilisate was shown to be equipotent against all test fungi in vitro. Mean while, SEM assay revealed that the lyophilisate induced detrimental effect on cellular membrane integrity as indicated by the formation of pores in all test fungi and the shrinkage of hyphal and microconidial structures in M. gypseum and T. mentagrophytes, possibly due to the leakage of essential intracellular components. The results open an opportunity for developing effective alternative antifungal agents, particularly in the face of the emergence of dug-resistant fungi.
  Tjandra Anggraeni , Nida Nisrine , Anggraini Barlian and Sony Heru Sumarsono
  Background and Objectives: Drosophila melanogaster is one of insect vectors for bacterium blood disease on banana plantation in Indonesia. The most common method to control these insect vectors is by using a synthetic insecticides, although it is now considered not to use as it causes pollutions. Alternatively, using essential oils that are environment friendly in order to stop insect visiting flower, as the fruits are self-fertilized. This research was carried out to identify the optimum concentration and the effective duration time for repellency of three essential oils from citronella, ginger and basil against D. melanogaster. Materials and Methods: By using a 5-arm test arena, each essential oil with the concentrations of 0 (control), 25, 50, 75 and 100% (with Tween-80 emulsifiers) was tested. Collected data was analyzed using a non-parametric test (Kruskal-Wallis), with a significant value p<0.05. GC-MS was used to identify the compound contents in each oil. Results: The result showed that the average number of D. melanogaster visits to the test arm with essential oils (25, 50, 75 and 100%) was significant different with control. The optimum concentration and the effective duration time for, repellency of each essential oil were as follows; citronella oil 75%-135 min, ginger oil 50%-90 min and basil oil 50%-105 min. Three highest concentrations of the compound in each essential oil were identified as follow: Citronella oil with citronellol, caryophyllene and geranyl isobutyrate, ginger oil with eucalyptol, camphene and geraniol; and basil oil with geraniol, bisabolene and sulcatone. These compound were presumably contributed in insect visiting. Conclusion: Essential oils from citronella, ginger and basil can be used as an alternative method to protect the banana plant from insect visiting flower and were effective to repel D. melanogaster with various optimum concentrations and various optimum duration times of repellency.
  Anggraini Barlian , Kusnandar Anggadiredja and Indah Prihatin
  Indonesia as tropical country is endemic to various infectious diseases, including that affecting the skin due to fungi infection. In nature, the eggshell, present as hard or soft shell, protects the embryo from microorganism infection. Turtle produces soft shells, which is very unique in that it does not contain albumin. In nature, the turtle eggshell has to cope with high humidity, so it is assumed that turtle soft shell has potent antifungal activity. This study investigated the potency of the eggshell extract from green sea turtle (Chelonia mydas) as an antifungal against Candida albicans, Trichophyton mentagrophytes, Microsporum gypseum and Aspergillus brasiliensis. Protein extraction was carried out with dialysis followed by lyophilization and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Antifungal activity assay was conducted by Dilution Plating method with nystatin as a standard drug. The possible mechanism of antifungal activity was assessed using Scanning Electron Microscopy (SEM). Fungal growth was inhibited by 8% w/v or 8x104 ppm extract, equivalent to 0.53 ppm nystatin against Candida albicans, 0.55 ppm against Microsporum gypseum, 0.36 ppm against Trichophyton mentagrophytes and 0.35 ppm against Aspergillus brasiliensis. The SDS-PAGE showed that the turtle eggshell extract had 6 proteins with molecular weight 20; 45, 50, 66, 80 and 116 kDa. This study showed that 8% w/v green turtle eggshell extract has inhibitory activity against Candida albicans, Trichophyton mentagrophytes, Microsporum gypseum and Aspergillus brasiliensis, as also proven by SEM results. Taken together, the present results showed that turtle eggshell extract has antifungal potency that could be developed as antifungal drug.
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