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Articles by Andrea BUNEA
Total Records ( 4 ) for Andrea BUNEA
  Francisc DULF , Sanda ANDREI , Cristian MATEA , Andrea BUNEA and Adela PINTEA
  The aim of the present study was to investigate the fatty acids, sterols and chlorophylls content and profile in leaves of apple cultivars (Malus domestica Borkh) which are intended to be cryopreserved. Quantitative GC-FID was used for phytosterols and fatty acids analysis, while chlorophylls were determined by a spectrophotometric method. Total phytosterols ranged from 189.6 to 463 mg/total lipid extract. The highest phytosterols content was found in Romus 3 and Romus 4 cultivars. The most important phytosterols in all leaves samples were: β-sitosterol, sitostanol, Δ5-avenasterol, 24-methylenecycloartenol and Δ7-stigmastenol. Some other minor sterols were also identified: campesterol, stigmasterol, citrostadienol. Fatty acids analysis revealed important quantitative differences, with a total content ranging from 77.4 mg/g to 245 mg/g. The major fatty acid in all samples was α-linolenic acid with 40 - 55 %, followed by palmitic acid with 17.5 - 25 %, linoleic acid 7 - 14 % and oleic acid 4.7 - 9.6 % of total fatty acids. Polyunsaturated fatty acids represent the major fraction, comprising 54-65 %. The ration between unsaturated and saturated fatty acids ranged between 2.1 - 3.2. The high degree of unsaturation is characteristic to green tissues. The chlorophyll a content ranged between 1.39 - 2.3 mg/g fresh weight, while chlorophyll b was found at 0.6 - 0.98 mg/g. The ratio chl a/chl b was close to 2.3 for all apple cultivars.
  Augusta LUJERDEAN and Andrea BUNEA
  It was analyzed lactoperoxidase activity in individual milk samples of 14 Friesian cows at 2, 14, 30, 90 and 180 days postpartum. Enzyme activity decreases from 0.75 U/ml in samples taken 2 days post partum to 0.36 U/ml 180 days postpartum. Lactoperoxidase activity was higher when analyze was performed 6 hours after milking (0,40 U/ml) compared to 48 hours (0.15 U/ml). Variation among cows was also large ranging from 0.54-0.94 U/ml on a specific sampling day. Such variations indicate that the efficiency of enzyme in preserving milk is affected by many factors including stage of lactation, individual animal and sampling day.
  Dumitrita RUGINA , Simona VICAS , Mariana PETRAN , Adela PINTEA , Andrea BUNEA and Carmen SOCACIU
  In Europe, mistletoe extract (Viscum album) is widely used as complementary cancer therapies. The main active component of mistletoe extract is glycoprotein, lectin, that exhibit cytotoxic effects and immunomodulatory activities. The aim of this study was to show if the mistletoe extract, rich in phenolic acids presents anti-carcinogenic potential on A2780 tumor ovarian cells. In the first part of this study, using high performance liquid chromatography (HPLC) analysis we characterized phenolic acid content of mistletoe growing on Malus domestica. And, in the second part, we investigated whether by apoptosis on ovarian tumor cell line A2780 if anti-cancer effect of this extract mistletoe is induced. Cell proliferation was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylt-etrazolium bromide (MTT) assay. The A2780 cell viability was inhibited to 87% by 50 μg/ml mistletoe extract treatment, and to 79% at 110 μg/ml extract concentration. To find out the apoptotic cell death, 4, 6-diamidino-2-phenylindole (DAPI) staining was performed. Cells treated with mistletoe (110 μg/ml) for 24 h displayed typical morphological features of apoptotic cells, with condensed and fragmented nuclei. Fluorimetric method was used to evaluate total reactive oxygen species content from A2780 ovarian tumor cells after mistletoe treatment. Mistletoe concentration 70μg/ml induces a significant toxic ROS-generating effect. These data indicates that the phenolic acids from mistletoe induce cell death by apoptosis on human tumor ovarian cell line, A2780.
  Zorita SCONTA , Dumitrita RUGINA , Floricuta RANGA , Adela PINTEA and Andrea BUNEA
  Different Romanian cultivars of Vaccinium corymbosum (Elliot, BlueCrop, and Duke) and Vaccinium myrtillius (Wild 1, Wild 2) were collected and analyzed for chlorogenic acid using High Performance Liquid Chromatography. Bluecrop cultivar was found to have the highest chlorogenic acid content (181.55 μg/100 g FW) among the cultivated blueberries analyzed, but the lowest values were obtained for Duke cultivar (97.84 μg/100 g FW). Both wild cultivars had high quantities of chlorogenic acid comparing to cultivate ones.
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