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Articles by Anbessa Dabassa
Total Records ( 8 ) for Anbessa Dabassa
  Anbessa Dabassa and Shiferaw Demissie
  Salmonelosis is a leading food borne disease worldwide and Africa at large. Eggs were one of the sources Salmonella for the food. So, this study was aimed to evaluate the traditional knowledge with scientific methods for detection of Salmonella in egg shell and content. Randomly collected egg samples were categorized based upon traditional knowledge and Salmonella metabolic recovery and proliferation of cells were done with primary and secondary enrichment media. From a total of 60 samples analyzed, 31 isolates of Salmonella were detected. Moreover, Conventional biochemical and serological test methods were used to identify the suspected Salmonella. The isolates were checked for their susceptibility to different antibiotics on Muller-Hinton agar in standardized inoculum and disc diffusion technique with 11 Oxoid drug discs. The result of study shows that 71% and 29% of Salmonella isolates were detected from egg shell and contents, respectively. All the isolates were resistant to ampicillin; Amoxicillin, Tetracycline and also 45% of the isolates were susceptible to Gentamicin and 16% for ciprofloxacin. Most Salmonella isolates show multi-drug resistance for at least five antibiotics. The laboratory results were supported the traditional egg spoilage identification knowledge, hence most of Salmonella isolates were detected from movable, floating and opaque eggs. On the other hand, the detection of high number of Salmonella isolate from the egg shell could be due to poor sanitation of the laid egg environment. Hence, the appropriate care and awareness should be needed before and/or after the egg laid to minimize contamination of egg by Salmonella.
  Teshome Tadesse and Anbessa Dabassa
  Salmonella sp. is one of the most commonly reported foods borne disease all over the world and developing countries at large. The cattle heath protection is the basic for production of microbiologically safe and sufficient milk and also preferable for consumption by human being. So that, antimicrobial resistant Salmonella were the big threat to public health concern. The increasing rate of antimicrobial resistance strains were main reason existing for aggravated bacterial disease. Thus, this study was done to indicate the frequency of antimicrobial resistance Salmonella isolates from rawcow’s milk in individual farmers and dairy farms of Kersa district that is ready for consumption. A cross sectional study was conducted by collecting rawmilk samples from dairy farms and individual farmers. Isolation and identification was made by serological and different traditional biochemical tests methods. The prevalence of Salmonella spp. in raw milk of the study area was 20%. The isolated Salmonella spp. were resistant to at least two or more antimicrobials which used in this study. Among tested drugs Nalidixic acid (80%) was most highly resistant; however, most susceptible to Ciprofloxacin (95%).So, the study was aimed to determine prevalence of antimicrobial resistance bacteria and to make the concerned bodies to take corrective measure.
  Shiferaw Demissie , Geda Kebede , Diriba Muleta and Anbessa Dabassa
  Awetu is the River which passes through Jimma town of south western Ethiopia, and one of the major water resources for irrigation and domestic activities for the area. Improper utility of contaminated water create unlimited health concern for the society that utilizing the water for farm and domestic activities. This study was important to evaluate the microbial load, protolytic and lipolytic activities of the isolated microorganisms. The results showed great number of bacterial contaminant in the river. Mainly, Aerobic mesophilic, Enterobacteriaceae, Staphylococcus and aerobic spore formers bacteria range between 103-106 CFU 100 mL-1 while yeast and mold ranged between <30 to 103. In a total of 30 samples were analyzed for microbial load determination using conventional culture method. The results of the this study indicate that the human and cattle feces, domestic activities, as well as solid and liquid waste disposal around the river were the main sources of contamination potential of water. Therefore, continuous and accurate assessment of the quality of water had a paramount importance. In addition, the presence of fecal contaminants in the three sites along the river segment indicates the problem of the water quality. Possible remedial actions are needed as recommendations in this study.
  Anbessa Dabassa
  Frequency of contamination in home slaughtered meat Jimma town south west Ethiopia was examined in the present study. The microbiological safety and quality issue is the pro-active measure to prepare the meat for consumption. A total 48 raw meat samples including beef (n = 4), Mutton (n = 37), chevron (n = 7) were collected from individual household and small food seller house in the town were analyzed for microbiological contamination using conventional culture methods. The bacterial spp. isolated from the samples were Bacillus, Micrococcus species, Salmonella species, Enterobacteriacea, coliform, Staphylococcus species and other gram positive rods, some of which harbor human pathogens of public health concern. The aerobic mesophilic counts varied from 0.19 to 3.67 log10 CFU g-1. Coliforms were encountered in all samples. Staphylococci were present at 0.95-2.28 log10 CFU g-1 in 36% (8) mutton and 50% (2) chevron, samples. Salmonella species were present in 10.42% (5.0) of all the samples. The majority of meat samples investigated in this study had contaminant microorganism and some pathogens. The present study revealed that despite very low contamination rate, foodstuffs particularly beef, chevon and mutton parts could be a potential vehicle for foodborne infections and implementation of preventive measures and consumer food safety education efforts are needed.
  Shiferaw Demissie and Anbessa Dabassa
  Water quality is the great public health concern in developing countries and for the study area at large. This study is aimed to evaluate the Physico-chemical and antibiotic resistance activities of some bacterial isolates obtained from Awetu River. In this study, 284 bacterial strains were isolated and evaluated for their antimicrobial resistance patterns. All of the isolates tested were not sensitive to Nor, Chl, Kan, Gen and Tet. Among the bacterial isolates, the most frequent resistance was noted for methicillin (96%), followed by resistance to penicillin (85%). The most frequent resistance among (Stapylonocci and Micrococci isolates was observed for Methicillin and Penicillin and streptomycin (96% each), followed by resistance to kanamycin (84%), gentamicin (55%) and methicillin (30%). A total of 9 Multiple Drug Resistance (MDR) patterns were detected. About 42% of the isolates showed MDR to four drugs, 29% to five drugs and 2% to six drugs. The most frequent MDR pattern was Van/Str/Kan/Gen/Met and was seen in 42% of the isolates. This pattern was seen in Lactobacillus, Pediococcus and Weissella isolates, but was the most frequent pattern in Pediococcus isolates (41%). The results obtained from physico-chemical and antimicrobial susceptibility patterns of the isolate were the health risk for the study area community. The study also found the coliform contamination to be the key problem with drinking water.
  Yihun Mola , Anbessa Dabassa and Shiferaw Demissie
  Bakery products are moracious and staple foods consumed among most of the world’s population. These products are prepared from cream, nut, nougat. These delicious foods can cause food borne diseases particularly halophile pathogenic microbes such as Staphylococcus aureus in case of poor hygienity. This study was aimed to evaluate the extent of cream filled bakery products contaminated with Staphylococcus aureus. A total of 30 samples were purchased from randomly selected cafeterias in Jimma town and transported to research and postgraduate laboratory of Biology department. A serial dilution was made accordingly and 0.1 mL aliquot sample was plated on pre-solidified Mannitol Salt Agar (MSA) and incubated at 35°C for 48 h. Samples were collected from three sites (Merkato, Agip and Kochi). The result of the study showed that the mean count (CFU g-1) of S. aureus was dominant (6.3±0.1) in cream cake followed by donut (4.5±0.2) while the lowest mean count was recorded in zebib (4.3±0.2). Most (73.3%) of the food samples were positive for S. aureus. The isolates of S. aureus were resistant to penicillin G, methicillin and oxacillin, however; 75% of isolates were sensitive to erythromycin. Generally, the microbial safety of cream filled bakery products was poor and needs special attention.
  Shelema Tulu , Shiferaw Demissie and Anbessa Dabassa
  Spices have been get Great attention among the world population because of its great value in food floor and aroma. Moreover it is benefiting for sellers by creating job opportunities. However it perceived to be major public health problem due to microbial contamination. The purpose of this study was to evaluate microbial safety of spices in Jimma town. The study involves laboratory analysis for microbiological safety of spice. The standard method were used for the enumeration of Aerobic mesophilic, Entrobacteriaceae, coli forms, Staphylococci, yeasts and molds. A total of 40 spice samples Red chilies (Capsicum spp.), Turmeric (Curcuma longa) were collected from Mercato, Kochi and Agip selling sites. This result show that the overall mean microbial counts (CFU g-1) were dominated by Aerobic mesophilic bacteria (7±o.4), yeasts (6±o.4), coliform (6±0.0), molds (6±0.0), Staphylococci (4±0.8) and entrobacteriaceae (5.5±0.5). Out of total 208 isolates characterized, the most dominant were Bacilli spp. (83.33%) followed by Staphylococci spp. (14.3%). Out of 20 mycopopulation isolates characterized, molds were dominant (75 %) and yeast were 25%. Among the molds isolates, Mucor spp. were predominant (41.6%) followed by Aspergillus spp. (16.6%) whereas Pencillium and Geotrichum spp. (12.5%) while Eurotium, Rhizopus and Fusarium spp. were least recorded fungi (8.33%). Spices contamination problem in the present study could be due to poor preparation, selling site, storage area and materials and poor personal hygiene. Generally, the microbial safety of spices sold in Jimma town was poor and call for special attention.
  Lata Lachisa and Anbessa Dabassa
  Composts and bio-fertilizers are the most important elements for agricultural development activities. In agricultural both practices are prominently improves plant growth. Besides, Composts and bio-fertilizers suppress plant disease, presence of a pathogen and a susceptible host in the environment. Plant disease suppression is the direct result activity of consortia antagonistic microorganisms and composted manure. Isolation and identification of the soil borne fungal pathogens and rhizosphere bacteria were done in vitro based on their morphological and biochemical examination; to evaluate effects of rhizosphere bacteria isolates and composted manure on Fusarium wilt of tomato/Lycopersicon esculentum/plants disease. Additionally, antagonism experiment likewise hydrogen cyanide test and plant growth promotion test (phosphate solubilization, starch hydrolysis and screening of Indole Acetic Acid (IAA)) productions were carrying out. Under greenhouse condition the disease severity, disease incidence and measurement of growth parameters were correlated using two rhizosphere isolates (RhB-7 = Rhizobacteria Bacillus spp., RhP-12 = Rhizobacteria Pseudomonas spp.) and two different percentages of composted manures (10 and 20%) including their combination. The result revealed 100% disease incidence and severity in control experiment and 29-100% efficacy in combined treatment. Furthermore, the combination of RhB-7, RhB-12 and 20% of composted manure were the most efficient against Fusarium wilt disease of tomato than the rest treatments. Through, rhizosphere bacteria isolates and composted manure it is possible to control tomato/Lycopersicon esculentum/plant wilt which is caused by the fungal called Fusarium spp. and increase the soil fertility and the product yields.
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