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Articles by Amsalu Abera
Total Records ( 2 ) for Amsalu Abera
  Amsalu Abera , Fikre Lemessa and Diriba Muleta
  This study was carried out with the objective of evaluating the antifungal potential of aqueous and ethanol extracts of eight different plant species in vitro and in vivo against Colletotrichum kahawae in completely randomized design with three replications. The extracts were from Hagenia abyssinica, Allium sativum, Phytolacca dodcandera, Croton macrostachyus, Maesa lanceolata, Eucalyptus globules, Eucalyptus citriodera and Lippia adoensis. Subsequently, two most effective plant extracts were tested in vivo against the disease on detached green coffee berries and seedling applying the extracts at 3 different times of application (at the time of inoculation and 48 h before and after inoculation) on the pathogen. The study indicated that the inhibitory effect of the extracts depended on the type of plant species used, method of extraction and time of application of the extracts. Generally, A. sativum and C. macrostachyus aqueous and ethanol extracts were the most effective plants that significantly reduced radial growth of the pathogen compared to the control. A. sativum reduced radial growth of the pathogen in ethanol and aqueous extracts by 83 and 100%, respectively and C. macrostchyus by 68 and 88%, respectively. Furthermore, A. sativum extracts consistently reduced disease severity on detached green berries and seedling in greenhouse at all times of application. Nevertheless, the efficacy of C. macrostachyus on detached green berries and seedlings was inconsistent and variable based on method of extraction and time of application of the extracts. The study indicated the possible use of extracts of A. sativum as an alternative means of CBD (coffee berry disease) management but further study at field conditions should be carried out to verify the result.
  Fikre Lemessa , Amsalu Abera , Girma Adunga and Weyessa Garedew
  Coffee (Coffea arabica L.) bean quality can be affected by a number of factors of which storage fungi are one of the major ones. In Ethiopia coffee is a number one export commodity supporting the national economy but there was little information about the association of mycoflora with coffee beans. Therefore, the objective of this study was to assess the associations of mycoflora with coffee seeds and their effect on coffee infection at Limmu Coffee Plantation. The comparison was done using two coffee berry disease resistant selections (74112 and 74110) with and without parchment and with and without surface treatment of coffee beans with 5% sodium hypochlorite and storing under two storage conditions (local cold house and corrugated iron warehouse). Thus, the experiment was laid down as 2×2×2×2 factorial experiment with four replications. The study showed the association of four fungal species (Fusarium spp., Aspergillus spp., Penicillium spp. and Mucor spp.) and some unidentified species in both blotting and agar plating techniques. In general, significantly higher infection percentage was found in coffee seeds without parchment and surface disinfection irrespective of the storage type and coffee selection. Thus, keeping coffee beans with parchment and disinfecting by disinfectants may reduce the association and prevalence of mycoflora on coffee and minimize postharvest problems.
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