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Articles by Amjad Khalil
Total Records ( 4 ) for Amjad Khalil
  Amjad Khalil , Dhia S. Hassawi and Abeer Kharma
  The effects of three Salvia species (S. officinalis, S. dominica and S. spinosa) on five bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Klebsiella sp. Escherichia coli and Enterobacter sp.) were investigated. Also, the genetic variation among the Salvia sp. was determined. The three Salvia sp. showed different antimicrobial activity in different extract amounts against the five bacteria. Salvia officinalis and Salvia dominica inhibited the growth of Staphylococcus aureus at all used extract amounts (200, 150, 100 and 50 mg mL-1). S. spinosa showed no antimicrobial activity. Based on variation of RAPD patterns obtained with four primers (B-7, B-10, B-12 and B-17), the similarity among Salvia sp. was determined. The UPGMA-based (unweighted pair group method of arithmetic means) dendogram was constructed between the three species by using the similarity matrix results.
  Amjad Khalil
  Essential oils or volatile oils from medicinal plants have potentially very strong antimicrobial activities for control of pathogenic microorganisms. Ocimum basilicum is one of the medicinal plants which are widely used as sources of extracts with strong antibacterial and antioxidant properties. In this study, the leaf extract of Ocimum basilicum was tested for its antibacterial activity against some human pathogenic bacteria (Staphylococcus aureus and Escherichia coli). Antimicrobial activity test was carried out by using the hole-plate diffusion method. Plant materials were dried and extracted with 95% ethanol. The tested extract showed very strong antimicrobial activity against both strains. The antimicrobial activity was evaluated by measuring the zone of inhibition. The strongest inhibition activity was observed against E. coli (21 mm zone) at 200 mg mL-1 of leaf extract followed by Staphylococcus aureus which showed 16 mm inhibition zone at 200 mg mL-1 of leaf extract. Physical properties of the extract of Ocimum basilicum were evaluated. The color of the extract is greenish, has intense scent, density is 0.937 g cm-3 at 25°C, refractive index is 1.489 and specific gravity is 0.887.
  Amjad Khalil and Basem F. Dababneh
  Investigation of the inhibitory effect of extracts from 4 medicinal plants were carried out against four plant pathogenic fungi: Rhizoctoria solani, Fusarium oxysporum, Vetricillum sp. and Penicillium sp. The highest growth inhibition of all fungi was observed with Varthemia iphionoides (E4), which gave (44.8%), of inhibition for Verticillium sp., followed by Rhizoctronia solani (42.9%), Fusarium oxysporum (42.7%) and Penicillin sp. (18.2%). All extracts showed the highest inhibitory effect against the plant fungi at 1000 ppm except the extract from Phlomis viscose (E2) which showed the highest inhibitory effect at 500 ppm.
  Basem F. Dababneh and Amjad Khalil
  The inhibitory effect of extracts from five Jordanian medicinal plants were studied against five plant pathogenic fungi: Crupina crupinastrum, Teucrium polium, Achillea santolina, Micromeria nervosa and Ballota philistaea. All plants showed antifungal activity against the fungi used in this study. The inhibitory effect on activity increased by increasing the concentration (from 100-1000 ppm). The highest growth inhibition of all fungi was found with Achillea santolina at 1000 ppm, which gave 42.2 and 42.0% of inhibition with Fusarium oxysporum and Rhizoctonia solani, respectively and the lowest were Micromeria nervosa and Ballota philistaea which gave 3.6 and 3.5%, respectively against pencillium sp. Results clearly indicate that the medicinal plants were used in this study are a promising source of antifungal compounds.
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