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Articles by Amit Sharma
Total Records ( 5 ) for Amit Sharma
  Amit Sharma , Pankaj Sood and Jitendra Kumar Chaudhary
  Background and Objective: Goats are assuming a greater importance under small household system in India. Season affects the fertility of semen and thereafter is an important factor limiting the large scale application of artificial insemination with frozen semen. Limited literature underlying the correlation of climatic parameters with seminal quality. Hence, the present study was designed to find out the correlation of temperature (°C) and sunshine (h) on processed semen quality in Gaddi and Chegu goats. Materials and Methods: A total of 286 ejaculates (Gaddi, n = 106; Chegu, n = 180) were collected using artificial vagina and selected on basis of standard quality parameters. The ejaculates were extended in Tris citrate egg yolk extender containing 10% EY and 6% glycerol to maintain a concentration of 150×106 spermatozoa/straws. The representative straws from each ejaculates were thawed at 37°C for 30 sec, 24 h post incubation to compare seminal parameters between fresh and post thaw semen along with percent change due to the processing. Results: The data was analyzed using package R version 3.4.3. In Gaddi goats, HOST reactivity was negatively and significantly correlated (r = -0.471, r = -0.527, p<0.01) to temperature in both fresh diluted and post thaw semen. Whereas, temperature was negatively significantly correlated (r = -0.745, r = -0.768, p<0.01), with post thaw progressive motility and viability in Chegu goats. Conclusion: In conclusions, present study delineates temperature rather than sunshine (h) affecting semen quality in both Gaddi and Chegu bucks during breeding season.
  Vinay Kumar Jadon , Amit Sharma and S.S. Yadav
  This study presents an approximate solution to a 3-dimensional free convection flow of a viscous, incompressible fluid past an impulsively started infinite, vertical porous limiting surface through porous medium with transverse sinusoidal suction in presence of uniform magnetic field. Using perturbation technique, the expressions for the transient velocity in the main flow direction, the temperature, the sinusoidal skin fraction and the rate of heat transfer have been discussed with there respective dependence on the Prandtl number (Pr), Grashoff number (Gr), Magnetic parameter (M) and Porosity parameter (K).
  Amit Sharma and Vijay Kumar
  In this study, researchers discuss the frequency modes (first two modes) of non-homogeneous isotropic square plate on C-C-S-S condition where C and S represent clamed and simply supported, respectively. Here, thickness varies linearly in one direction. For non homogneity in the material, we considered circular varation in density. Bi-parabolic temperature variation on the plate is also being viewed. To find the vibrational frequency, Rayleigh Ritz technique has been applied for the different values of plate’s parameters.
  Amit Sharma
  A mathematical model is presented to study the effect of circular variation in density (as a non-homogeneity parameter) and linear variation in thickness to natural vibration of square plate on clamped (C-C-C-C) boundary condition under temperature enviornment. It is considered that the temperature variation along both the axes are parabolic. To obtain frequency modes, Rayleigh Ritz technique has been applied. All the results are presented in the form of tables. Comparison of the result with existing result (in literature) is also presented in the form of figures.
  Ashwani Sharma , M. Yogavel , Reetesh Raj Akhouri , Jasmita Gill and Amit Sharma
  Malaria parasite UIS3 (up-regulated in infective sporozoites gene 3) is essential for sporozoite development in infected hepatocytes. UIS3 encodes for a membrane protein that is localized to the parasite parasitophorous vacuolar membrane in infected hepatocytes. We describe here 2.5-Å resolution crystal structure of Plasmodium falciparum UIS3 soluble domain (PfUIS3130-229) in complex with the lipid phosphatidylethanolamine (PE). PfUIS3130-229 is a novel, compact, and all α-helical structure bound to one molecule of PE. The PfUIS3130-229-PE complex structure reveals a novel binding site with specific interactions between PfUIS3130-229 and the PE head group. One acyl chain of PE wraps around part of PfUIS3130-229 and docks onto a hydrophobic channel. We additionally provide new structural and biochemical evidence of PfUIS3130-229 interactions with lipids (phosphatidylethanolamine), with phospholipid liposomes, and with the human liver fatty acid-binding protein. The direct interaction of PfUIS3130-229 with liver fatty acid-binding protein most likely provides the parasite with a conduit for importing essential fatty acids/lipids. Therefore, our analyses have implications for lipid transport into the parasite during the rapid growth phases of sporozoites. Given that PfUIS3 is essential for establishment of liver stage infection by P. falciparum, our data provide a new target for abrogating parasite development within liver cells before typical symptoms of malaria can manifest.
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