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Articles by Amer Jamil
Total Records ( 5 ) for Amer Jamil
  Nighat Aslam , Farooq Latif , Sibtain Ahmed and Amer Jamil
  Trichoderma harzianum (E-58) was grown on Vogel`s medium containing glucose and xylan as carbon source. Glucose repressed the β-glucosidase production while xylan produced the enzyme in substantial amounts. Total RNA was isolated from mycelia of Trichoderma harzianum grown in medium containing xylan as carbon source. RT-PCR was performed on the isolated RNA. cDNA strand was synthesized by using oligo (dT)18 primer. Specific primers were used to amplify bgl gene from cDNA. The bgl gene was ligated at SmaI site of pUC18 and transformed into E. coli.
  Aamira Iqbal , Haq Nawaz Bhatti , Sofia Nosheen , Amer Jamil and Muhammad Ashraf Malik
  Present study shows the complexity of determining the chemical composition of three exopolysaccharides obtained from bacteria associated with plant roots grown under salt stress conditions. Physicochemical analysis indicated that the exopolysaccharides were varying in their chemical composition. Presence of the sugar shows that these were hetero/homo polysaccharides. IR analysis of the crude extracts for the presence of functional groups indicated a need for more purification of exopolysaccharides. Moreover the IR analysis along with histochemical studies showed the presence of hydroxyl, carboxyl and nitro groups. The physicochemical characteristics of the exopolysaccharides showed that the exopolysaccharides would be used in industrial applications. However, a thorough investigation is needed to determine their industrial application.
  Asma Nafeesa , Munir Ahmad Sheikh , Ikram-ul-Haq , Amer Jamil and Zahida Parveen
  A total of 60 isolates of Staphylococcus aureus, 30 each from upper respiratory tract as well as from post operative wound infections were subjected to antibiogram studies. Staph. aureus isolates from upper respiratory tract were found 30, 53.33 and 43.33% resistant against ciprofloxacin, amoxycillin and chloramphenicol respectively. A similar pattern of antibiotic resistance was observed against the bacterial isolates from post operative sepsis. Susceptibility of ciprofloxacin, amoxycillin and chloramphenicol in such cases was found only to be 43.33, 10 and 3.33% respectively. The high bacterial resistance appears to be due to indiscriminate use of antibiotics.
  Asma Nafeesa , Tahira Iqbal , Munir A. Shiekh , M. Nawaz and Amer Jamil
  The biodisposition kinetin and urinary excretion of kanamycin was investigated in eight normal adult female goats of beetle breed after intravenous close of 5 mg/kg to each gear. After single intravenous administration of kanamycin. in goats, the distribution half-life and biological hall-life had average of 8.1±0.893 min and 2.87±0.12 hrs and ranged between 4.28 to 11.61minutes and 2.44 to 3.37 hrs respectively. The aapparent volume of distribution of kanamycin in goat calculated by area method was 584.6±34.21 mg/kg. The rate constants for Lananivein transfer across compartment 1 to 2, K12 and 2 to 1, K21 were 1.846±0.316 hr–1 and 3.800±0.434 hr and ranged between 1.016 to 3.713 hr–1 and 2.45 to 5.77 hr–1, respectively. Total body clearance of kanarnycin calculated on per kg basis ranged 1.79 to 3.59 ml/min/kg with an average value 2.389±0.194 ml/min/kg, Cumulative urinary excretion as percent of total does excreated at 12 hours was 10.266±1.233. The studies showed variation in pharmacokinetic behaviour of kanamycia in indigenous goats as compared to earlier studies.
  Sibtain Ahmed , Qurrat-ul-Ain , Nighat Aslam , Saima Naeem , Sajjad-ur-Rahman and Amer Jamil
  Xylanases and cellulases are industrially important enzymes obtained from various microorganisms. Trichoderma harzianum is a preferred microorganism for the production of complete xylanases and cellulases. E-58 strain of the fungus was grown in different media including Enzyme Production Medium (EPM) and Vogel`s medium with different carbon sources like xylan, glucose and CMC to get the optimal production of xylanases and cellulases. It was found that glucose repressed the synthesis of these enzymes whereas xylan and CMC produced these enzymes in substantial amounts. Maximal activity of cellulases and xylanases was marked at pH 5.5 and at temperature 28°C. The fungus was grown for 5 days at 120 rpm in orbital shaker. Maximum enzyme activity of xylanase was found to be 1.209 IU mL-1 whereas maximum activity of cellulases (exoglucanase, endoglucanase and β-glucosidase) was found to be 2.764, 14.4 and 1.1209 IU mL-1 respectively.
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