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Articles by Amarjit Singh
Total Records ( 1 ) for Amarjit Singh
  Anamika Gupta , Geeta Devi Leishangthem , Kuldip Gupta , Naresh Kumar Sood and Amarjit Singh
  Background: The present study was undertaken to investigate the expression of Th1 and Th2 cytokines and to correlate the expression of these cytokines with pathological changes associated with natural cases of specific and non-specific lymphadenopathies in bovines. Materials and Methods: The study included 32 cases of bovine lymphadenopathies presented for necropsy examination to the Department of Veterinary Pathology, GADVASU, Ludhiana, India. Samples from affected lymph nodes and visceral organs showing lesions were collected for cytology, histopathology, immunohistochemistry, ELISA and PCR. Results: There was variable expression of Th1 and Th2 cytokines in specific and non-specific lymphadenopathies in bovines. In lymphoma the expression of both Th1 and Th2 cytokines was decreased. There was increased expression of IFN-γ in bovine tuberculosis and strong IL-10 expression in Johne’s disease. In non-specific conditions, there was continuation of both Th1 and Th2 responses in acute lymphadenitis, along with increased Th2 expression in cases of hyperplasia, hemorrhagic and chronic lymphadenitis. Conclusion: The present study concluded that analysis of cytokine expression and pathological lesions in the lymph nodes of bovines are of paramount importance in diagnosis of specific and non-specific lymphadenopathies.
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