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Articles by Amal A.H. Saleh
Total Records ( 3 ) for Amal A.H. Saleh
  Amal A.H. Saleh
  The impact of the combined stress factors of heavy metals (cadmium and nickel) and UVA+B radiation in Pisum sativum was investigated. Seeds were grown in sandy loam for 30 days and then randomly divided into equal two groups. The first group was exposed for 5 h day-1 to supplemental 6.4 KJ. dose UVA+B radiation beside white light (1100 Lux), while the second one was exposed to white light only giving light intensity (1100 Lux). Plants from each group irrigated with nutrient solution having 0.0, 5, 10 and 20 ╬╝mol Cd or Ni for two weeks and then harvested. The results showed that, Cd or Ni treatments induced significant decreased the length, fresh weight, dry weight and density of both root and shoot. UVA+B resulted in synergistic inhibitory effects on all measured growth parameters. Total chlorophyll, carotenoid, total carbohydrates and proteins showed a similar pattern to that of growth parameters. The amount of UV-screening pigments and anthocyanin was highest under the supplemental UVA+B radiation, while the combination of Cd or Ni and UVA+B decreased them. From this result, it showed that the combined treatment of UVA+B and Cd or Ni showed an antagonistic effects on UV-screening pigments. The combined stress of UVA+B radiation and Cd or Ni also resulted in a reduction of peroxidase and catalase activity, while increasing of H2O2 level in Pisum sativun plant. The data showed that in some measured parameters was a UVA+B and Cd or Ni synergistic effect, while with other parameters an antagonistic action was observed.
  Amal A.H. Saleh
  The present study was carried out to study the effect of chilling stress on some metabolic products and antioxidative system in mung bean (Vigna radiata L.) plant and try to alleviate chilling injuries by using paclobutrazol, abscisic acid and H2O2. Twenty-day-old seedlings were randomly separated into equal three groups, the first group was left in green house at 35°C, the second one was chilled at 5°C for 5 or 10 h and the last one was treated with 0.0, 25 and 50 mg L-1 paclobutrazol, 0.5 and 1 mM abscisic acid or 0.0, 0.1 and 0.5 mM H2O2 before transforming to 5°C. The results showed that, chilling stress induced significant increased lipid peroxidation, membrane leakage and hydrogen peroxide level, while activities of catalase, peroxidase and ascorbate peroxidase were decreased. In addition, total chlorophyll, total carbohydrates, protein content and proline level decreased following exposure to 5°C. Paclobutrazol, abscisic acid and H2O2 treatments ameliorated the chilling injuries by lowering lipid peroxidation, membrane leakage and H2O2 level and increasing in total chlorophyll, carbohydrates, protein content, proline level and antioxidant enzymes activities. Based on these results, it was presumed that the stress protection caused by paclobutrazol, abscisic acid and H2O2 probably contributes to some extent to the enhanced activity of the free-radical scavenging systems.
  Amal A.H. Saleh , Dina Z. Abdel-Kader and Amr M. El Elish
  The objective of the present study was to examine the role of heat shock and salicylic acid in antioxidant homeostasis in mungbean (Vigna radiata L.) plant. Fifteen-day mungbean seedlings were divided into 6 groups. The first one kept in open air then harvested after 25 and 32 day post germination (negative control).The second one were exposed to 50°C for 3 h (heat stress) and then harvested after 10 days (positive control). The 3rd and 4th groups were exposed to 2 different heat shock temperature (40 and 45°C) for 1, 1.5 and 2 h. The fifth and sixth groups were sprayed with two different Salicylic Acid (SA) concentrations (0.5 or 1 mM). The groups from third to sixth were exposed to heat stress (50°C) for 3 h either directly or after week. The seedlings were harvested 10 days post heat stress. The results showed that, high temperature stress induced lipid peroxidation, electrolyte leakage and increased hydrogen peroxidase level. High temperature stress decreased catalase, peroxidase, ascorbic peroxidase activities and glutathione content. It was also found that high temperature stress induced a significant increase in endogenous SA concentration and superoxide dismutase activity. These deteriorative symptoms in the mung bean seedlings were ameliorated by heat shock treatments or SA application by decreasing lipid peroxidation and increasing catalase, peroxidase, ascorbic peroxidase, superoxide dismutase activities and glutathione content. Based on these results, it was presumed that the stress protection caused by heat shock treatments or SA application contributes to some extent to the enhanced activity of the free-radical scavenging systems.
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