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Articles by Alper Baran
Total Records ( 2 ) for Alper Baran
  Handan Aydin , Alper Baran , Abit Aktas , Kamber Demir , Elif Sahin , Abdullah Kayar , Serhat Pabuccuoglu and Suzan Daglioglu
  Two, 4-Dichlorophenol (2, 4-DCP), an environmental pollutant has been in agriculture and synthetic chemical industry. The aim of present study was to analyse the testicular toxicity of 2, 4-DCP, which caused biochemical, spermatological and histological changes in male mice and to evaluate the possible ameliorative effect of soybean extract and L-Tryptophan (L-TRP). Soybean extract (25 mg/kg bw/day) and L-TRP (150 mg kg-2 bw/day) were given by intraperitoneal (ip) route for 14 days. 2, 4-DCP was administered to male mice with drinking water at dose of 1000 ppm for 14 days. Biochemical parameters in serum ((glucose, creatinine, Blood Urea Nitrogen (BUN), Aspartate Transaminase (AST), Alanine Transaminase (ALT), Lactate Dehydrogenase (LDH)), spermatological and histological changes were investigated at the end of the 14 days comparatively with control group. We conclude that soy extract and L-tryptophan alleviate 2, 4-DCP testicular toxicity.
  Alper Baran , Kamber Demir , B. Evrim Sahin , Mithat Evecen , Suleyman Bacinoglu , Serhat Alkan and Irfan Kamuran Ileri
  The aim of this study was to observe the effects of taurine containing and non-containing milk extenders on the spermatological characteristics of chilled stored male cat semen. Semen was collected from 4 cats of 2-3 ages under general anesthesia (xylazine-ketamine HCl) by an electro-ejaculator during the study. Total 5 ejaculates with a weekly basis were collected from each male cat. The semen was split into 2 portions and one was extended with taurine containing Skimmed Milk-Glucose (SMG-T) extender at 26°C (egg yolk (10 mL)-D (+) glucose (1.00 g)-taurine (50 mM)-skimmed milk (to 100 mL)-penicillin-G (1000 IU mL-1)-streptomycin sulphate (1000 μg mL-1)) the other portion was extended with Skimmed Milk-Glucose extender (SMG) (egg yolk (10 mL)-D (+) glucose (1.00 g)-skimmed milk (to 100 mL)-penicillin-G (1000 IU mL-1)-streptomycin sulphate (1000 μg mL-1)). The extended semen samples were chilled to 5°C in an hour (0.35°C min-1). Spermatological investigations such as motility (%) and abnormal spermatozoon rates (%) (acrosome, head, mid-piece, tail and total) were done at dilution, cooling to 24 h, 48 h and 72 h at 5°C. Morphological defect rates were examined by staining with Spermac® stain by a light microscope at 1000 magnification. The mean motility of cooled semen in the SMG-T extender at the 24 h was the highest with 75.50±5.82% and this value was 66.25±12.86% in the SMG extender (p<0.05). During the above time period, the mean acrosome and total abnormal spermatozoon rates in the SMG-T extender were lowest with 18.55±3.15 and 32.70±4.59%, respectively, these averages were highest in the SMG extender with 19.60±5.67 and 40.15±9.10%, respectively (p<0.05). The motility and morphologic defect rates at the 48 h were similar in the SMG-T and SMG extenders. At the 72 h, acrosome and tail defect rates in the SMG-T extender were lowest with 33.50±4.36 and 15.65±6.26%, respectively whilst, these values were highest in the SMG extender with 40.45±8.76 and 17.65±3.84%, respectively (p<0.05). At the conclusion of the study, it can be said that SMG-T and SMG extenders can be successfully employed to store cat semen for shorter periods (24-48 h), also, SMG-T extender is superior to the SMG extender in cooled storage of cat semen for 24 h.
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