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Articles by Alma Nunez Del Arco
Total Records ( 2 ) for Alma Nunez Del Arco
  Marisela Leal-Hernandez , Enrique Salas Tellez , Alma Nunez Del Arco , Rodolfo A. Ugalde , Victor R. Tenorio-Gutierrez , Rigoberto Hernandez-Castro , Francisco Suarez Guemes and Efren Diaz-Aparicio
  The aim of this research was to evaluate the humoral and cellular response, produced in mice inoculated with Brucella ovis and mutants in virB10 and virB11, measuring IgG1, IgG2a, IgG2b and IgM levels and cytokines production IL-2, IL-4 e IFN-γ, in lymphocyte culture. Mice of 6-weeks-old Balb/c were immunized intraperitoneally with each strain of B. ovis and the mutants with 3x1013 CFU mL-1 in PBS. For the cellular response, they were injected 2 times. In the humoral response the predominant antibodies were IgG2b and IgM in the different strains. The cytokine production with the wild type were indicative of Th2 and low response of IFN-γ and IL-2, that is indicative of an answer Th1 type characteristic of intracellular bacteria. The Reo 198 strain, showed Th1 type response. The mutant ΔvirB11 only presented response to IFN-γ 24 h that decreases after 48 h and with the mutant virB10:: Gm the shown levels of the different cytokine were low, this is indicative that the genes virB10 and virB11 of B. ovis need are complete to induce a suitable immune response. In the virulence in mice only recovered the Reo and wild type strains until 1 week postinoculation and the mutants can recover until 4 and 5 weeks postinoculation. One concludes the immune response by the different strains of B. ovis was moderate and can be attributed to that the murine model not was the more recommended to evaluate to cellular response would presumably have been specific to specie by the poorly or inability response showed when were mice inoculated with the different strains of B. ovis.
  Alma Nunez del Arco , Enrique Salas Tellez , Efren Diaz Aparicio , Miguel Angel Hernandez Balboa , Victor Hugo Abrego Reyes and Enrique Angeles Anguiano
  The use of sophisticated techniques for the analysis and identification of microorganisms is more and more common; such is the case of capillary zone electrophoresis technique, which allows a quick separation and identification of microorganisms. Actinobacillus seminis is a bacteria involved in ovine epididymitis, a disease common to this species and currently, its identification is solely bacteriological and is conducted through a microbiological analysis, which is confusing and slow, since there is not specific biochemical test that distinguishes this bacterium from other bacteria that are also involved in ovine epididymitis, such as Brucella ovis, mainly. This work presents pre-treatment results and run conditions of Actinobacillus seminis for its identification through capillary zone electrophoresis.
 
 
 
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