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Articles by Ali Nasrolahi
Total Records ( 2 ) for Ali Nasrolahi
  Amin Mokhlesi , Soodabeh Saeidnia , Ahmad Reza Gohari , Ahmad Reza Shahverdi , Ali Nasrolahi , Farzaneh Farahani , Reza Khoshnood and Nasrin Es`haghi
  Sea cucumbers are potentially presented as the marine sources of antimicrobial and cytotoxic compounds. In this study, we focused on the sea cucumber, Holothuria leucospilota which has not been evaluated until now for antibacterial, antifungal and toxicity of its main organs. Ethyl acetate, methanol and water-methanol extracts were prepared from cuvierian organ, coelomic fluid and body wall of H. leucospilota collected from Persian Gulf. Extracts were evaluated for their antibacterial and antifungal activities against Aspergillus niger, Candida albicans, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli. The activity was determined using the disc diffusion test. Cytotoxic activities of the extracts were assessed by Artemia salina larvae. Results showed that methanol extract of cuvierian organ (Minimum inhibitory concentration (MIC/disc) <1 mg against A. niger) and water-methanol extract of coelomic fluid (MIC/disc = 1 mg against C. albicans) showed significant antifungal activities but no inhibitory effect of the extracts against bacteria was observed. MIC for water-methanol extract of coelomic fluid was 3 mg against both C. albicans and A. niger. Significant inhibitory effect was observed in cytotoxic assays which was highest in body wall methanol extract (LC50 = 0.4 μg mL-1).
  Ali Nasrolahi
  The effect of different food concentration, substratum, light periodicity, salinity, cyprid density and cyprid age on the settlement of Balanus improvisus were studies. Released larvae were mass reared in 0.6 L glass beakers to the cyprid stage. Then, attachment was monitored once a day. Chaetoceros calcitrans was used as food at three cell concentrations (0.5x105, 1x105 and 2x105 cells mL-1). Larval settlement was carried out on two substratum under three light period (12L:12D, 16L:8D and 8L:16D) at seven salinities (7, 12, 15, 18, 25, 28 and 36 ppt). Cyprid density was used from five to 200 larvae per 5 mL of seawater. Cyprids were aged for one to seven days. The maximum and minimum percent settlement was observed at 2x105 and 0.5x105 cells mL-1, respectively. Cyprids settled in higher numbers on polystyrene compared to glass surface. Percent settlement was higher in 8L:16D than another light periods but, no significant difference in percent settlement between 12L:12D and 16L:8D light periods was seen. Differences in settlement at various salinities were not statistically significant except in 10 ppt which percent settlement was significantly higher. Cyprid density did not have any effect on percent settlement but cyprid age had a significant effect on percent settlement.
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