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Articles by Ali Osman Belduz
Total Records ( 2 ) for Ali Osman Belduz
  Yusuf Bektas and Ali Osman Belduz
  Morphological variation among Turkish populations of the Atlantic horse mackerel Trachurus trachurus (Carangidae) was examined for 150 specimens at five different sampling localities (Sea of Marmara (Istanbul and Canakkale), Aegean (Izmir) and Northeastern mediterranean (Antalya and Iskenderun) seas) in Turkey by discriminant function analysis of eleven morphometric and five meristic characters. The results of discriminant function analysis showed that there is limited gene flow among T. trachurus populations in Turkish coastal waters. The Marmara Sea sample was similar to the Aegean Sea samples for both morphometric and meristic data. Morphometric and meristic data showed the heterogeneity between Aegean and Mediterranean mackerel samples. The Mediterranean samples separated from all others and indicate 2 local populations (Iskenderun and Antalya) for morphometric data, unlike meristic data that revealed no significant differentiation between 2 Mediterranean localities. Discriminant function analyses indicated that morphometric differentiation between the samples was largely due to differences in the head characters of fish. It is confirmed that metric characters are much less effective for discriminating between T. trachurus populations than morphometric characters, which is expressed higher distances between samples. The results indicate a small degree of spatial separation in morphology in T. trachurus among the studied localities.
  Yusuf Bektas and Ali Osman Belduz
  PCR based nuclear and mitochondrial DNA markers are used to clarify cases of misidentification of adult individuals of fish species with morphological similarities. This problem prevails in the case of false scad (Caranx rhonchus) overlap geographically and has similar morphology with Turkish species of the genus Trachurus. Hence, the nuclear DNA/mitochondrial DNA approach was used for C. rhonchus, to identify and discriminate, it from Trachurus sp. coexist. Polymerase Chain Reaction (PCR) amplification of the nuclear 5S rDNA gene is a suitable method for differentiating between C. rhonchus and Trachurus sp. On the other hand, a PCR-RFLP technique, which involves digesting of the whole mitochondrial cytochrome b gene with XcmI and BsrGI restriction enzymes to generate DNA profiles, was used to identify of C. rhonchus and discriminate it from three Trachurus sp. This PCR-RFLP analysis is simple, rapid and reliable and hence can be regularly used to discover fraudulent substitution among economically important C. rhonchus and Trachurus sp. Results have confirmed the usefulness of these techniques to distinguish and genetically identification of morphologically similar carangid species.
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