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Articles by Ali H. Bahkali
Total Records ( 6 ) for Ali H. Bahkali
  Wafa`a A. Al-Taisan , Ali H. Bahkali , Abdallah M. Elgorban and Mohamed A. El-Metwally
  Sclerotinia sclerotiorum is a destructive pathogen of several economically important crops, including soybean, pea, bean, canola and sunflower. This pathogen exhibits little host singularity and has a host range that includes more than 400 primarily dicotyledonous plant species. In this study, the inhibitory effects of 5 essential oils and 4 microelements against S. sclerotiorum were examined. Cinnamon, clove and mint oils completely inhibited the in vivo mycelial growth of the fungus at all concentrations. In addition, iron exhibited a marked antimicrobial effect against S. sclerotiorum. A soil application containing cinnamon oil significantly reduced the incidence of disease caused by S. sclerotiorum, producing 75% plant survival compared to the control. Calcium also significantly reduced the disease incidence that giving 80% living plants.
  Ashraf A. Hatamleh , Ali H. Bahkali , Mohamed El- Sheshtawi , Abdallah M. Elgorban and Mohamed A. El- Metwally
  In the present study, the antifungal activity of Cinnamomum zeylanicum Blume, Trigonella foenum-graecum L., Eucalyptus globulus Labill, Eruca sativa L. and Allium sativum L. extracts were investigated against soil borne pathogenic fungi. The results showed the plant extracts had inhibitory activities on the mycelial growth and spore germination of these fungi. The results exhibited C. zeylanicum and E. globulus at 1000 ppm gave the highest inhibition on the mycelial growth of the tested fungi except Fusarium verticillioides (Sacc.) that giving 48.5 and 54.4% inhibition, respectively. Whereas, A. sativum was the best extract effective against F. verticillioides (F. moniliforme) which produced 55.9% reduction in the mycelial growth. Extract of C. zeylanicum completely inhibited spore germination of the three Fusarium species tested. Also, E. globulus completely inhibited spore germination of F. oxysporum f. sp. melonis (Leach. and Currence) Snyd. and Hans. While, A. sativum gave 100% inhibition of the spore germination of F. solani at 1000 ppm concentration.
  Abdallah M. Elgorban , Ali H. Bahkali , Mohamed A. El- Metwally , Mohamed Elsheshtawi and Mohamed A. Abdel- Wahab
  In the present study the antimicrobial activity of essential oils of allium bulb (Allium cepa L.), black cumin seeds (Nigella sativa L.) and eucalyptus (Eucalyptus globulus Labill) were evaluated against five fungi (Fusarium oxysporum f.sp. melonis, Fusarium solani, Fusarium verticillioides, Sclerotinia sclerotiorum and Rhizoctonia solani). Allium cepa oils completely inhibited the mycelial growth of Fusarium oxysporum f.sp. melonis, Fusarium solani and Sclerotinia sclerotiorum at 500 ppm concentration. While, E. globulus oil completely inhibited the radial growth of F. solani, S. sclerotiorum R. solani. On the other hand, the percentage inhibition varied in case of Nigella sativa oil is 28.6-73.9% from (F. oxysporum f.sp. melonis, F. verticillioides) at 500 ppm concentration. Whereas, the spore germination of F. oxysporum f.sp. melonis and F. solani were completely inhibited by the application of A. cepa oil, while the oil of eucalyptus completely inhibited the spore germination of F. solani and highly effective against spore germination of F. oxysporum f.sp. melonis, F. verticillioides with 98 and 93%, respectively at 500 ppm concentration. Considering the inhibition in the growth and spore germination, it concluded that allium and eucalyptus essential oils could be used as possible bio-fungicides alternative to synthetic fungicides against phytopathogenic fungi.
  Ali H. Bahkali , Abdallah M. Elgorban , Abd El-Rahim M.A. El- Samawaty , Huda-Mogren A. Almogren , Mohamed A. El- Metwally and Naif S. Al-Harbi
  Aspergillus species are regularly involved in human broncho-pulmonary diseases, mainly in immunocompromised patients. The essential oils extracted from three different plants were tested for their inhibitory effect on the growth of five pathogenic Aspergilli including Aspergillus flavus, Aspergillus fumigatus, Aspergillus niger, Aspergillus versicolor and Aspergillus terreus which are pathogens with clinical importance. The results of the antimicrobial tests showed that the isolated essential oils inhibited the growth of Aspergillus species to varying degrees. The Ocimum basilicum oil completely inhibited the mycelial growth and spore germination of A. terreus and A. fumigatus at 200 and 250 ppm. In addition, this oil showed a very strong antifungal activity against the mycelial growth and spore germination of A. flavus and A. versicolor with 97.7, 97.5, 91.2 and 93.5%, respectively, when compared with control. The essential oil extracted from Eucalyptus globulus also exhibited significant antifungal activity against the Aspergillus species tested. This oil completely inhibited the mycelial growth and spore germination of A. fumigatus. Conversely, Nigella sativa oil exhibited moderate inhibitory activity against all the tested Aspergillus species. The present study supports the proposition that plant essential oils may have a role in both pharmaceutical and agricultural practices.
  Shaban R.M. Sayed , Ali H. Bahkali , Marwa M. Bakri , Abdurahman H. Hirad , Abdallah M. Elgorban and Mohamed A. El- Metwally
  In the present study, Aspergillus terreus used for the biosynthesis of silver nanoparticle. The A. terreus cell free filtrate reacted with silver nitrate, resulting formation of silver nanoparticles (AgNPs). The AgNP was marked by visual analysis, UV-Vis absorption spectroscopy, Transmission Electron Microscopy (TEM) and Scanning Electron Microscopy (SEM). The AgNP showed high absorbance at 450 nm in UV-Vis spectroscopy. The TEM micrograph exhibited poly disperse spherical and ellipsoid NPs in the size range from 20-50 nm. The fungus biogenic AgNPs established strong antibacterial activity against Staphylococcus aureus (MRSA), Shigella boydii, Acinetobacter baumanni, Shigella sonnei and Salmonella typhimurium compared with four antibiotics used. Biological way using the microorganisms is a new approach to the secure, less expensive and eco-friendly technique for the synthesis of silver nanoparticles is gaining importance in the field of nano-biotechnology.
  Ali H. Bahkali
  The growth of phytopathogenic fungus Verticillium tricorpus on 15 carbohydrate substrates was monitored to analyse the range of polysaccharide-degrading enzymes and glucosidases production. The secretions of endo and exoenzymes, capable of degrading cellulosic, hemicellulosic and pectinolytic polysaccharide were observed during the course of the experiment. Pectinolytic activities were produced constitutively on all of the substrate tested, while cellulolytic enzymes were to induced in simple sugar (i.e., glucose or xylose) media. Polysaccharide growth substrates and cellulase inducers increased all of the enzyme activities tested.
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