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Articles by Ali Abbas Qazilbash
Total Records ( 5 ) for Ali Abbas Qazilbash
  Ali Abbas Qazilbash , Rani Faryal , Kokab Batool Naqvi , Safia Ahmad and Abdul Hameed
  This study was designed to ascertain the effectiveness of isolated indigenous Bacillus species to remove chromium from industrially polluted effluents, through a series of effluent biotreatment regimen. Using microbiological techniques, 6 Bacillus strains were isolated, characterized and labeled QIP 1-6. All strains displayed maximum growth on media with a Cr concentration of 400 ppm. Strains QIP 1 and 5 showed maximum resistance to chromium showing moderate growth at 900 ppm of Cr. Following optimization, strain QIP 1 removed 46.81% of the Cr6+ and QIP 5 removed 42.50% of the chromium at the same concentration over the same incubation period, as determined through atomic absorption spectroscopy. Following different biotreatment regimens, biotreatment C1-filtered effluent inoculated with strain QIP 1, incubated under optimum conditions, showed maximum Cr6+ removal (10.29%) after 48 h. Interestingly, the second highest removal of Cr6+ (8.94%) occurred in the untreated, unfiltered raw effluent (D) after incubation for 48 h. Significant (p<0.05) strong positive correlation patterns emerged between these two regimen, as well as between regimen B 1-filtered effluent inoculated with strain QIP 1, but incubated under un-optimized conditions-and regimen D. The study revealed that intrinsic bioremediation does occur naturally and that it is quite possible for a consortium of Bacillus species to work more effectively at removing the HM from the contaminated sites, than a single isolate, thereby reducing the labor intensive work involved.
  Ali Abbas Qazilbash , Rani Faryal , Ayesha Tabasum , Asima Lodhi , Safia Ahmad and Abdul Hameed
  Over the years, with the active spread and development of the industries, heavy metals, which are either used, or produced, as by-products, by numerous manufacturing, industrial, refining and mining processes, have become ubiquitous, persistent environmental pollutants. Bioremediation is a cost-effective technology that may be used to remove a variety of pollutants and contaminated sites. This study was designed to compare the capabilities of identified Aspergillus niger strains in the oven-dried (dead) and non-growing (living) forms to adsorb Lead (Pb) from SDB media containing the HM under optimized laboratory conditions using the shake-flask incubation method. Aspergillus niger showed a higher percentage of Pb adsorption in the oven-dried, as compared to the non-growing state. Furthermore, the adsorption by oven-dried strains was also high at higher concentrations of the heavy metal, whereas adsorption of Pb effectively took place at the low concentration levels for the non-growing strains. Analysis of the correlation matrices showed significant positive correlation between strains at high Pb concentrations under the oven-dried condition and significant positive correlation at low Pb concentrations under the non-growing conditions. The percentage Pb adsorption of both strains under oven-dried conditions was over 90% for the concentration range of 100-500 ppm, with the highest being 99.95% at 500 ppm for strain NP 18 after 80 min of incubation. Furthermore, for the non-growing strains this maximum adsorption was achieved for the concentration range of 100-300 ppm and that too was 96.37% achieved by strain NP 17 at 300 ppm concentration after 30 min, after which the toxic effects of the HM on the live fungal strains diminished their capacity to adsorb the metal. When comparing the two biotreatment regimens of oven-dried fungal biomass, verses the use of non-growing pellets of fungi, there is overwhelming evidence to support the use of the former as a method to removal HM contamination from polluted sites, particularly at high concentration levels.
  Sofia Khanum , Noor-us-Saba , Mazhar Qayyum , Badar ul Islam and Ali Abbas Qazilbash
  This study was designed to determine the prevalence of Salmonella sp. in 300 clinical samples (stool and blood) isolated from salmonellosis suspected patients. Samples were cultured on Salmonella-Shigella, MacConkey, agars enriched with Selenite F broth. Biochemical and serological confirmatory tests were carried. Salmonella sp. were found to be the principal etiological agent in 22 (7.3%) cases. Only 2 serotypes Salmonella typhi (63.6%) and Salmonella paratyphi A (36.4%) were reported. Prevalence rate was greater in blood (81.8%) than stool samples (18.2%), with higher Salmonella typhi (85.7%) than Salmonella paratyphi A (75%) isolated from positive blood samples. Incidence rates of salmonellosis were slightly higher among females as compared to males (54.5 and 45.5%, respectively), with equal gender distribution of Salmonella typhi cases, but a higher incidence of Salmonella paratyphi A among females (62.5%) than males. Of the Salmonella positive cases, 63.6% were among the 5-14 years age group, followed by 13.6% in the less than 5 years age group. Of the Salmonella typhi cases 71.4% were reported in children aged 5-14 years and 7.1% in infants under 5 years of age. For Salmonella paratyphi 50% of cases were reported among children in the 5-14 years age category and 25% in children under the age of 5 years. Fever was the most common symptom (81.8%), followed by chills (75%), abdominal pain (68%), vomiting (59%) and diarrhea (54.5%). Highest number of Salmonella typhi cases (42.9%) were reported among those living on the poverty line and 28.5% living in below the poverty line, whereas 50% of the cases of Salmonella paratyphi, reported in this study, were among those living in extreme poverty. Of the positive cases 58.8% of the children had illiterate parents. Of the children with Salmonella typhi 54.4% had illiterate parents. Of the children with Salmonella paratyphi, 65.7% had parents who lacked any sort of formal education. Among the adult population positive for Salmonella infection, 40% of them were uneducated. Of those with Salmonella typhi 66.6% were uneducated, while 50% of Salmonella paratyphi adult cases were among those who had received no formal education. In Rawalpindi, the highest incidence of salmonellosis was reported among patients residing near the Nullah Leh (35.3%). Of those patients residing in the Nullah Leh, 36.4% and 33.3% had Salmonella typhi and Salmonella paratyphi as their etiological agent, respectively. While 18.2% and 33.3% of the Salmonella typhi and Salmonella paratyphi cases, respectively, were reported from rural areas surrounding Rawalpindi. For Islamabad only a total of 5 cases of Salmonella infections were reported.
  Uzma Asghar , Noor-us- Saba , Abdus Samad and Ali Abbas Qazilbash
  The study was designed to determine the incidence of Salmonella and Shigella spp. in blood and stool samples of patients. A total of 150, samples of blood (110) and stool (40) were collected from patients, reporting at NIH (National Institute Health) and six Salmonella and two Shigella strains were identified by colony identification and biochemical tests and serotyping. Of the 6 Salmonella isolates; 2 were of S. typhi, 1 of S. paratyphi A, 1 of S. paratyphi B and 2 of other Salmonella spp. The 2 Shigella strains from stool samples were S. flexneri (Poly B serotype). Salmonella isolates were found to be susceptible to gentamicin, ofloxacin, cefotaxime, amikacin, tobramycin, cefaclor, while resistant to ampicillin, cefamendole, chloramphenicol, gentamicin and cefuroxime. Shigella strains showed resistance to vibramycin, ampicillin, tetracycline and sensitivity against nalidixic acid, norfloxacin, chloramphenicol, amikacin, and aztreonam. Despite the fact that the overall prevalence of Salmonella and Shigella reported in this study was low, there is sufficient evidences to indicate that better hygiene and water treatment and management would even further reduce the incidence of these infections.
  Madahiah- bint-e-Masood , Noor-us- Saba , Abdul Samad and Ali Abbas Qazilbash
  Klebsiella pneumoniae a human commensal is sometimes associated with urinary tract infection (UTI) due to long-term catherization, wound infections, bacterimia and nosocomial infections. This study was designed to determine the incidence of Klebsiella pneumoniae among UTI patients and included 152 urine samples. Of the 152 samples, 6 (3.95%) showed Klebsiella pneumoniae to be the causative agent. Furthermore, there was no predominating pattern in the sex-wise distribution, as of these 6 cases, 3 were females and 3 males. The antibiogram pattern showed that amikacin (100%), norfloxacin (100%) and pipemidic acid (100%) were the most effective drugs, as all the isolates were sensitive to them, whereas all the isolates were found to be resistant to ampicillin and 67% were resistant to nitrofurantin and augmentin.
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