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Articles by Ali A. Shati
Total Records ( 5 ) for Ali A. Shati
  Mohammad Y. Alfaifi , SeragEldin I. Elbehairi , Ali A. Shati , Usama A. Fahmy , Nabil A. Alhakamy and Shadab Md
  Background and Objective: Role of dietary phenolic compounds in the modification of pathophysiological conditions in cancer is immense. Thus, ellagic acid (EA), a natural polyphenolic compound has been recognized for its anticancer activity in different preclinical studies. However, clinical application is limited because of its poor aqueous solubility and thereby, inadequate oral bioavailability. The present work was aimed to formulate micellar delivery, an effective nano-delivery tool for EA, using D-α-tocopheryl polyethylene glycol succinate (TPGS) by film-hydration method. Materials and Methods: Film hydration method was introduced to encapsulate EA within the TPGS micellar structure, which was then characterized and evaluated for in vitro release study. EA-TPGS micelle was further exposed to OVACR3 to determine cancer protecting potential of the formulation. Results: The delivery system (EA-TPGS micelles) consists of spherical shape of 113.2±23 nm with 0.260±0.038 PDI and drug-encapsulation efficiency of 88.67%±3.21. In vitro release profile in the phosphate-buffer saline was found to observe sustained pattern with 67.8% cumulative release within 12 h. Further, a dose dependent cytotoxicity of EA-TPGS micelles was observed on OVACR3 cells with an IC50 value of 12.36 μM. EA-TPGS significantly reduced viable cells via arrest of G1 phase of cell cycle and thereby induce apoptosis probably through inhibiting p15 and p21. Decreased fluorescence unit in ROS determination assay also reflected potential antioxidant activity of the EA-TPGS. Conclusion: These findings strengthened that use of EA-TPGS micelles could overcome the limitations in delivery of hydrophobic chemotherapeutics through oral route.
  Mohammad Y. Alfaifi , Ali A. Shati , Mohammed Ali Alshehri , Serag Eldin I. Elbehairi , Usama A. Fahmy and Ohoud Yahya Alshehri
  Background and Objective: Reports revealed atorvastatin (ATR) exerts significant anti proliferative properties against cancer cell lines. Aim of this study was the potentiation of ATR cytotoxic effects against HepG2 cells by using TPGS as a surfactant and loading on PLGA NPs. Materials and Methods: Nanoprecipitation method was used to trap ATR within PLGA nanoparticles, the method uses vitamin E TPGS as an emulsifier. The nanoparticles’ encapsulation efficiency, size, zeta potential (ZP), surface morphology and in vitro drug release were evaluated. Results: The mean size of the particles was 176.2±14.1 nm, they had a ZP of -15.1±4.4 mV and polydispersity index of 0.32 and after 12 h, approximately 96% of raw ATR had dissolved in comparing with 25% of raw ATR. It was found that ATR-NPs was twice as cytotoxic as the raw ATR. In both instances, for HepG2 cells cycle analysis accumulated at the pre-G1 apoptotic phase in response to ATR and ATR-NPs. Indeed, increase of cells at pre-G1 initiated by ATR 4.45 times the normal level, rising to a 24.05-fold increase for ATR-NPS. The ATR annexin V-FITC apoptosis assay showed significant increase in the amount of annexin V-FITC positive apoptotic cells (both early and late apoptosis) in HepG2 cells treated with ATR-NPs. Conclusion: These findings suggested that this formula is a promising therapy for patients with liver cancer.
  Mohammad Y. Alfaifi , Ali A. Shati , Serag Eldin I. Elbehairi , Usama A. Fahmy , Nabil A. Alhakamy and Shadab Md.
  Background and Objective: Febuxostat (FBX) is a proven prophylactic agent in the management of tumor lysis syndrome in patients with malignant tumors. The clinical application of FBX is hindered due to poor solubility and bioavailability. The purpose of the present study was to develop miceller delivery of FBX using an amphiphilic and non-ionic macromolecule, D-α-tocopheryl polyethylene glycol 1000 succinate (TPGS) for the treatment of lung cancer. Materials and Methods: FBX-loaded TPGS micelles (TPGS-FBX-M) were formulated by thin-film hydration method and are characterized for particle size and morphology using dynamic light scattering techniques and transmission electron microscopy. Developed miceller formulation of FBX (TPGS-FBX-M) was further evaluated for entrapment efficiency and in vitro release. Results: Nano-sized spherical miceller deliveries of TPGS-FBX-M displayed a release of 72.7% within 12 h. The formulation was employed to assess cytotoxicity in adenocarcinomic human alveolar basal epithelial cells (A549), where TPGS-FBX-M micelles were found to reduce IC50 values as compared to free FBX which might be due to increased uptake of FBX and effectiveness against A549 cells. An increased cell cycle blockade at the G2/M phase improved apoptotic activity and a significant increase in the expression of caspase 3 for TPGS-FBX-M as compared to free FBX incubation in vitro. Conclusion: This impressive drug delivery platform for lipophilic agents can also be used to deliver other hydrophobic chemotherapeutics to target cancer cells for improves efficacy and safety.
  Ali A. Shati
  As Aseer region showed the highest rate of human schistosomiasis in Saudi Arabia in the past few years, it is necessary to investigate the temporal and spatial variations in patterns of infection and the factors may have led to such heterogeneity in this region. Therefore, Generalised Linear Models (GLMs) were used to study the temporal and spatial variations in the prevalence of human schistosomiasis in two areas in Aseer region: Abha and Tihamah over eight years period (2000-2007). In addition, the contribution of various biotic and abiotic factors to the prevalence of the infection was estimated. Data of 1004953 people (682982 from Abha and 321971 from Tihamah) examined for infection were used in the analysis. In general, there was a decline in the prevalence of schistosomiasis over the study period and the prevalence of infection in Tihamah was significantly less than it was in Abha. Prevalence of schistosomiasis in Abha but not in Tihamah was significantly affected by seasons as people in Abha had the highest infection rate in summer. Schistosomiasis infection rate was affected by host sex as males had higher infection rate than females. Age group of 15-35 showed the highest infection rate. Infection rate was also positively correlated with snail infection rate. Prevalence of schistosomiasis in non-Saudi people was significantly higher than it was in Saudi people. This study showed that many abiotic and biotic factors have contributed to human schistosomiasis in Aseer region. There also was a spatial variation in the prevalence of schistosomiasis in this region as it was higher Abha than it was in Tihamah. Therefore, more attention should be paid to Abha area in the future schistosome control programmes beside the intensive control programmes being applied to Tihamah.
  Fahmy G. Elsaid , Ali A. Shati and Elsayed E. Hafez
  Aluminium (Al) affects the antioxidant/oxidant balance and several enzymes relevant to neurotoxicity. Natural products such as saffron and green coffee may have a pharmacological role in ameliorating the neurotoxicity induced by Al as a result of their antioxidant properties. The study aims to investigate the role of saffron and green coffee extracts in ameliorating the toxicity induced by aluminium chloride in the nervous tissues of rats. Sprague dawley rats were used in this study and divided into 6 groups: control group; Green coffee group (600 mg/kg b.w./day for 90 days); Saffron group,(200 mg/kg b.w./day; AlCl3 group,(40mg/kg b.w./day of AlCl3 for 90 days); AlCl3 plus green coffee group, co-administered with 40 mg/kg b.w./day of AlCl3 and green coffee extract at 600 mg/kg b.w./day; AlCl3 plus saffron group,(40 mg/kg b.w./day) of AlCl3 and aqueous saffron extract (200 mg/kg b.w./day). There was a decrease in the AChE activity in rats administered with AlCl3 for 90 days. Additionally, there was a decrease in the enzymatic antioxidant activities in both cerebral hemisphere and cerebellum of rats in AlCl3 group. At the molecular level, the expression of A Disintegrin And Metalloprotease (ADAM10), acetylcholinesterase, P53, Bcl-2 (B cell lymphoma 2) and interleukins (IL-4 and IL-12) genes in AlCl3 group was changed. Both green coffee and saffron extracts could be used to attenuate the neurotoxic effects of AlCl3 as the imbalance in antioxidants/oxidants system and in some genes expression.
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