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by
Alfred S. Traore |
Total Records (
6 ) for
Alfred S. Traore |
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C. Zongo
,
Etienne-Francois O. Akomo
,
A. Savadogo
,
Louis Clement Obame
,
Jean Koudou
and
Alfred S. Traore
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The antimicrobial activity of the total alkaloids from
the leaves of Mitragyna inermis (Willd.) O. Kuntze (Rubiaceae)
From Burkina Faso was evaluated using disc diffusion essay and broth microdilution
assay. The extract was used against ten (10) reference bacterial strains
and three (3) clinical isolates including Gram(+) and Gram(-) strains.
The alkaloids showed moderate activity against mcroorganisms tested. The
highest Diameter of Inhibition Zone (DIZ) was obtained with S. aureus
ATCC9244 (23 ± 1 mm). The lowest Minimum Inhibitory Concentration
(MIC) obtained was 0.625 mg mL-1 recorded with three (3) Gram(+)
bacteria (S. aureus ATCC 25293, S. aureus (clinical isolate)
and S. carmorum LMG 13567) and one Gram(-) strain (P.
mirabilis CIP104588. Results showed that Gram(+) bacteria are more
sensitive to alkaloids from M. inermis than Gram(-) bacteria. This
study confirmed the use of the plant in traditional medicine against some
infectious diseases. |
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L. Ouattara
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J. Koudou
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C. Zongo
,
N. Barro
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A. Savadogo
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I.H.N. Bassole
,
A.S. Ouattara
and
Alfred S. Traore
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The main goal of this study was to determine the phenol content, the antibacterial and the antioxidant activities of the three species of Lannea largely use in traditional medicine in Burkina Faso. The total phenolic and flavonoid contents of hydro alcoholic extract (70%V/V ethanol/distilled water) from the barks of Lannea acida, Lannea microcarpa and Lannea velutina (Anacardiaceae) were determined by the method of Folin Ciocalteu and AlCl3 by spectrophotometry. These extracts were tested for their antioxidant and antibacterial activities. Antioxidant activity was determined by the method of DPPH and compared with quercetin. Antibacterial activity was performed by disk diffusion and broth microdilution essays against nine reference bacterial strains including gram-positive and gram-negative bacteria. L.acida exhibited the highest total phenolic contents (40.55±0.26 g GAE/100 g) which correlated with better antioxidant activity (IC50 = 345.72±7.76 μg mL-1). Furthermore the highest content of total flavonoids (11.02±0.04 g QE/100 g) and the largest anti bacterial spectrum (7.82 μg mL-1≤MIC≤ 62.5 μg mL-1) were recorded with L.velutina. These results show that the barks of L. acida and L.velutina could be used respectively as a potential natural antioxidant and antibacterial agent. |
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Paul W. Savadogo
,
Aly Savadogo
,
Aboubacar S. Ouattara
,
Michel P. Sedogo
and
Alfred S. Traore
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Biodegradation of Sumithion (o,o-dimethyl o-(3-methyl-4-nitrophenyl) phosphorothioate) was tested using microorganisms isolated from mud under controlled conditions. Results showed that after 2 years of acclimatation, the indigenous microorganisms can biodegrade sumithion under anaerobic conditions. This biodegradation lead to methane production. Biodegradation kinetics monitored by high performance liquid chromatography and gas chromatography showed that the acclimated consortium is able to carry out the biodegradation of 20, 50 and 100 mg L-1 of Sumithion, respectively in 15, 25 and 45 days. The end products of sumithion biodegradation were gases. Such as CO2, N2, CH4 and H2S. Isolation and partial characterization of a strain able to degrade Sumithion were realized. This strain named SY can be easily produced and used for the bioremediation of waters and soils contaminated by Sumithion. |
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Simplice D. Karou
,
Denise P. Ilboudo
,
Wendyame M.C. Nadembega
,
Yaovi Ameyapoh
,
Djeneba Ouermi
,
Salvatore Pignatelli
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Virginio Pietra
,
Alfred S. Traore
,
Comlan de Souza
and
Jacques Simpore
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The present study aimed to ascertain for the current situation of antimicrobial resistance of major urinary tract bacteria in Saint Camille Medical Centre. During two consecutive years, 794 urine specimens were analyzed for microorganism isolation and identification. The microorganisms were identified by conventional methods used in the centre and antimicrobial assays were performed by the NCCLS agar disk diffusion. Pathogenic microorganism’s isolation was attempted for 89.04% samples. Escherichia coli (32.76%) was the most frequently isolated microorganism followed by Staphylococcus aureus (22.74%) and Klebsiella pneumoniae (10.45%). The antimicrobial screenings revealed very high antimicrobial resistance, to β-lactams. The resistance rates recorded with E. coli were 76.64, 74.01, 25 and 74.34% for ampicillin, amoxicillin amoxicillin/clavulanic acid and trimethoprime-sulfamethoxazole, respectively. Microorganisms were still susceptible to quinolones however, attention should be paid, because, the resistance rate already reached 10% for nalidixic acid and ciprofloxacin. Periodic performance of prevalence studies is a useful tool to know the current situation of microorganisms and their resistance patterns in an institution and it helps to access the emergence and the spread of antibiotic resistance. |
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Denise Ilboudo
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Damintoti Karou
,
Wendyame M.C. Nadembega
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Aly Savadogo
,
Ouermi Djeneba Salvatore Pignatelli
,
Virginio Pietra
,
Augustin Bere
,
Jacques Simpore
and
Alfred S. Traore
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The aims of this research are: i) to evaluate the prevalence of HHV-8, HBV and HIV among pregnant women, ii) to determine the percentage of these co-infections and iii) to estimate the frequency of the mother-to-child transmission of HIV among HBV and HHV-8 positive mothers. Thus, 379 pregnant women attending ante-natal consultation in Saint Camille Medical Centre were subject to HIV, HHV-8 antibodies and the viral marker Hepatitis B Surface Antigen (HBsAg) detection. We observed 48/379 (12.66%) HIV seropositive subjects. Among them, HIV-1 type infection was predominant (95.83%), only 2/48 (4.17%) subjects had a dual HIV-1 type and HIV-2 type infection, no single HIV-2 type infection was detected. 38/379 (10.02%) subjects were infected by HHV-8 and 30/379 (7.91%) were HBsAg positive. HHV-8 and HIV Co-infections rates were high within HBV positive patients and we had respectively 20.00 and 16.67%. 10.42% HIV positive women were co-infected by HBV while 12.50% were infected by HHV-8. Then, 15.79% subjects HHV-8 positive were co-infected by HBV or HIV. In spite of the PMTCT protocol application, five (10.42%) HIV positive women transmitted the virus to their children. Two HIV positive mothers were co-infected by HHV-8 and one by HBV. Among the 5 HIV infected, one mother (20.0%) was HBV positive and two (40.0%) HHV-8 positive. Although we did not have a large sample which would show large prélalences of the infections, we could put forward that the Co-infection of the HIV with one of these viruses (HBV or HHV-8) could favorite the mother-to-child transmission. |
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Fidele Tiendrebeogo
,
V.S. Edgar Traore
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Nicolas Barro
,
Alfred S. Traore
,
Gnissa Konate
and
Oumar Traore
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The aetiology of a disease characterized by severe leaf
curling and leaf distortion symptoms in peppers was studied in Burkina
Faso. Surveys were conducted in fields planted with sweet pepper (Capsicum
annuum) and hot pepper (Capsicum frutescens) to collect leaf
samples and assess disease incidence. Collected samples were analyzed
using biological tests (mechanical inoculation and whitefly transmission),
triple-antibody-sandwich- enzyme linked-immunosorbent assay (TAS-ELISA),
Polymerase Chain Reaction (PCR) and sequence analysis. Mechanical inoculation
tests were negative while transmission by the whitefly Bemisia tabaci
led to infection rates of more than 80%. TAS-ELISA and PCR tests indicated
that symptoms observed were associated with begomovirus infections. Involved
virus isolates showed pairwise nucleotide identity of more than 99% with
Pepper yellow vein mali virus (PepYVMV) and formed with this virus
a distinct clade in phylogenetic analyses. Altogether, these results indicated
that PepYVMV was the causal agent of leaf curling symptoms in peppers. |
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