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Articles by Akhmaloka
Total Records ( 1 ) for Akhmaloka
  Pingkan Aditiawati , Akhmaloka , Dea Indriani Astuti , Sugilubin and Megga Ratnasari Pikoli
  Coal as fuel should be necessarily pre-treated by desulfurization in order to prevent excessive emissions of sulfur dioxide, a precursor of acid rain. Organic sulfur in coal can be eliminated by microbial action through the technology known as biodesulfurization. Source of microorganisms in the present study was coal mine soil in which microorganisms have been adapted to use the sulfur in coal. Coal mine in South Sumatra was choosen as source of microorganisms in this study, because it is an area in Indonesia with the largest of subbituminous coal reserves. The microorganisms were activated as mixed culture by culturing the soil sample in mineral salt medium containing subbituminous coal as the sole sulfur. Desulfurization activities were examined by using three variations of the initial coal concentration, i.e., 10, 15 and 20% weight per volume. Growth and activity of the mixed culture on the subbituminous coal were monitored by measuring of medium pH, cell concentration, sulfate and organic sulfur concentration. The result showed that desulfurization activity of the mixed culture on 15% of coal was able to reduce sulfur up to 82.36%. Isolation and identification of the mixed culture based on genotypic and phenotypic characterizations revealed that members of the mixed culture were identified as genera of Enterobacter, Lelcersia and Bacillus. Observation on growth curves showing that the culturable isolates grew in at least three overlapping stages when using coal as sulfur source suggested that the members of the consortium worked alternately on coal as substrate.
 
 
 
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