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Articles by Ahmed M. Abd El Tawab
Total Records ( 2 ) for Ahmed M. Abd El Tawab
  Ahmed M. Abd El Tawab , H.A. Murad , Mostafa S.A. Khattab and H.H. Azzaz
  Background and Objectives: Enhancing poor quality roughages by biological treatments has interested for many researches in last years. So, in the current study was carried out to production of tannase enzyme by Aspergillus terreus and its impact on ruminal fermentation, degradability and gas production were evaluated through in vitro trials. Materials and Methods: Aspergillus terreus was grown as stand cultures in 100 mL conical flasks containing tannic acid powder medium. The maximum production of tannase by Aspergillus terreus was achieved at inoculum ratio of 4% (v/v), 72 h of incubation period, initial pH 5.0, urea as a nitrogen sources at a concentration of 0.33 g N L1 and pomegranate peel as a carbon source at a concentration of 10% (w/v). For animal feeding experiments in vitro dry matter, NDF, ADF, cellulose and hemicellulose disappearance and rumen fermentation were determined for the experimental diets. The concentrate:roughages ratio was 50:50 on DM basis, experimental diets plus different levels of tannase enzyme being 0, 5250, 10500, 15750, 21000 and 26250 IU kg1 DM were studied. Results: Tannase enzyme increased DM, NDF, ADF, cellulose and hemicellulose degradability of the treated diets compared with the control diet (0.0 IU kg1 DM), which gave the lowest values of diet degradability parameters. The maximum produced tannase efficiency percentage for dry matter degradability was obtained at 15750 IU kg1 DM. Production of tannase enzyme by Aspergillus terreus strain under solid state fermentation was superior over tannase production from other fungal strains under the submerged cultures. Conclusion: In conclusion tannase enzyme had positive improving of feed digestion in the current in vitro study.
  Mostafa S.A. Khattab , H.H. Azzaz , Ahmed M. Abd El Tawab and Hussein A. Murad
  Background and Objective: Cellulases enzymes are widely be interested due to its capability to degrade lignocellulosic materials. The current study was concerning on investigating different factors of cellulase production from fungal sources by using agricultural wastes and studying its impacts on ruminal digestion and fermentation. Materials and Methods: The study tested the cellulase enzyme production ability of fungal strains against different lignocellulosic. Simultaneously the effect of different fungal strains to choose according to the superiority of cellulase production, then environmental factors were studied such as carbon source concentrations, inoculum size, incubation period, initial pH and nitrogen source. Finally, produced cellulase was evaluated using in vitro batch culture technique. Results: Penicillium chrysogenum recorded the highest value for cellulase activity. Pea pods showed best carbon source with 17.5% concentration for cellulase production. About 4% inoculum size, 2 days of incubation and pH5 were recorded the highest value for cellulase production. In vitro fermentation results showed improvement of DM digestibility compared with control. Conclusion: The current findings showed potential possibilities to utilize agricultural wastes as a substrate for producing cellulase enzyme from Penicillium chrysogenum fugal strain which could be an effective additive to improve ruminant diet digestion and utilization.
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