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Articles by Aftab Alam
Total Records ( 6 ) for Aftab Alam
  Hasan Soliman Yusufoglu , Aftab Alam and Ahmed Mohamed Zaghloul
  Two Cycloartane saponins, astragaloside-IV and cyclocephaloside-I, were isolated from the roots of Astragalus gummifera. Their structures were determined by utilizing 1D and 2D NMR experiments. Hepatoprotective effects of astragaloside-IV against carbon tetrachloride (CCl4) induced nephrotoxicity and hepatotoxicity in rats was studied. The rats (200-230 g) of either sex were divided into 6 groups (6 rats each). Injection of a single dose of CCl4 increases the level of serum AST, ALT, GGT, ALP and total bilirubin activities, decreases non-protein sulfhydryls (NP-SH) and total proteins (TPs), increases malondialdehyde (MDA) and produces changes in hepatic and nephritic tissue structure. Administration of doses of 25, 50 and 75 mg kg-1 of astragaloside-IV reversed significantly (p<0.001) the liver diagnostic blood markers as well as stricture of nephrotoxicity and hepatotoxicity of the treated groups when compared to the control group. The present finding indicated that astragaloside-IV is an active hepatoprotective agent that restores the affected biochemical markers and nephritic and hepatic tissues.
  Ahmed I. Foudah , Aftab Alam , Gamal A. Soliman , Mohammed Ayman Salkini , Elmutasim O. Ibnouf Ahmed and Hasan S. Yusufoglu
  The study was designed to investigate the pharmacognostic parameters of the aerial parts as well as antimicrobial and antioxidant activities of methanol extract of Pulicaria somalensis O. Hoffm (MEPS). These studies were carried with a view to justify the future use of this plant. This study deals with the morphological, microscopical studies of leaf, aerial part of P. somalensis, along with the physicochemical and preliminary phytochemical analyses that were also studied. The antibacterial activities were tested against both Gram-positive and Gram-negative bacteria while antifungal activities were tested against Aspergillus niger and Candida albicans using the agar disc diffusion method. The zone of inhibition was compared with standard (Ampicillin). The study of in vitro antioxidant was performed by using DPPH and FRAP assays. The results of the present morphological study correlated with earlier reports. The microscopical and physicochemical finding explores the useful identification character for authentication of this plant. The preliminary phytochemical study showed the presence of phenol, tannins and flavonoids types of active drugs. The marked antibacterial, antifungal and antioxidant were observed, which may be due to the presence of active constituents present in MEPS. The outcome of the present findings suggested that the plant may be a good source of antibacterial and antioxidant. The present finding concluded the in vitro activities, so it needs to be explored more in vivo evaluation before adding it into the world of medicinal plants.
  Saleh Alqasoumi , Hasan Yusufoglu , Abdullah Farraj and Aftab Alam
  This study was designed to investigate the protective effect of 6-shogaol and 6-gingerol against hepatotoxicity induced by diclofenac sodium (DFS). Compounds 6-shogaol and 6-gingerol were isolated from 5% gingerol oleoresin and their potential hepatoprotective activity was evaluated. Hepatotoxicity was induced in rats by an intra-peritoneal (i.p.) injection of DFS (150 mg kg-1). Rats were i.p. injected with 6-shogaol and 6-gingerol (10 mg kg-1) for 6 days before induction of hepatotoxicity. Blood and liver sample were taken from each rat at 24 h post intoxication. Serum activity of liver marker enzymes such as alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were assayed. Levels of alkaline phosphates enzymes (ALP) and total bilirubin in serum and malondialdehyde (MDA) in the liver homogenate were also estimated. Moreover, liver injury was assayed histologically. Results of present study revealed that i.p. injection of DFS to rats induced hepatic damaged that was manifested by significant (p<0.001) increase in the AST, ALT, ALP, total bilirubin in serum and MDA in liver homogenate. Histological data presented marked damaged in section of liver from DFS treated rats. I.p. injection of 6-shogaol to rats for 6 days before DFS-intoxication reversed these altered parameters near to normal control values. On the other hand, 6-gingerol had comparatively low hepatoprotective efficacy.
  Hasan S. Yusufoglu , Gamal A. Soliman , Ahmed I. Foudah , Maged S. Abdulkader , Mohammed H. Alqarni , Aftab Alam and Mohammad Ayman Salkini
  Background and Objective: Arnebia hispidissima (A. hispidissima) a member of the family Boraginaceae, is dye yielding and medicinally important plant. It is widely used in the cosmetic industries. This study has been made for the preliminary standardization of A. hispidissima plant. Methodology: The standardization evaluation comprises of powder microscopy and fluorescence analysis and TLC profiling. In addition, preliminary phytochemical screening, determination of total phenol and in vitro free radical scavenging activity (DPPH radicals scavenging assay and reducing power activity) were preformed utilizing the methanol extract. Results: A microscopic study of powder of whole plant of A. hispidissima showed different types of trichomes, vessel and fibres. Fluorescence analysis showed different colours under visible light, low UV and high UV. TLC of the hexane extract developed 8, 6, 9 and 10 spots with visible light, low UV, high UV and ninhydrin-H2SO4 spray, respectively. The phytochemical analysis of the methanol extract gave a positive indication for the presence of active compounds including alkaloids, carbohydrates, glycosides, steroids, triterpenoids saponins, phenols, tannins, flavonoids and proteins. The quantitative analysis showed the presence of a significant quantity of total phenol and the in vitro antioxidant activity clearly showed the terrific antioxidant property. Conclusion: The data generated from the present study can be utilized for the identification and quality control of A. hispidissima plant.
  Hasan Soliman Yusufoglu , Gamal Abdulhakim Soliman , Ahmed Ibrahim Foudah , Maged Saad Abdulkader , Mohd Nazam Ansari , Aftab Alam and Mohammad Ayman Salkini
  Background and Objective: Silene villosa (Family-Caryophyllaceae) is used traditionally in different part of Asian continents for the treatment of various illnesses. Recently, S. villosa has been tested and confirmed for anti-inflammatory and antioxidant activities. Therefore, the present study was carried out to evaluate cardiac and renal protective effects of S. villosa extract (SVE) against CCl4-induced toxicity. Materials and Methods: The herbs of S. villosa were collected, authenticated, coarsely powdered and extracted in Methanol using percolation method. Wistar albino rats (≈200 g) of either sex were randomly divided into five groups. Group I (normal control) and Group II (toxic control) received normal saline (1 mL/100 g, p.o.) for 7 days. Groups III (positive control), IV and V (Test groups) were received Silymarin (10 mg kg–1, p.o.), SVE at doses of 250 and 500 mg kg–1, p.o., respectively for 7 days. On the 8th day, except normal group (Group I), all Groups were administered with CCl4 (1.0 mL kg–1, i.p). After 24 h of CCl4 administration, the serum cardiac function parameters such as (LDH, CK and Albumin) and the serum renal function parameters such as creatinine, urea and uric acid, electrolytes and tissue total proteins were measured as a marker of cardiac and renal toxicity. The cardiac and renal tissue parameters such MDA and NP-SH were measured for the in vivo antioxidant activities. Further, the biochemical studies were followed by histopathological studies of heart and kidney tissues. Results: The treatment of CCl4 in rats, significantly altered the cardiac and kidney function test limitation. Administration of SVE at two different doses of 250 and 500 mg kg–1 with CCl4 showed a significant (p<0.01-0.001) protective ability against CCl4 intoxication by repairing the cardiac and kidney function abnormalities. The protective ability of SVE was further confirmed by the histological study of cardiac and renal tissues. Conclusion: The results led us to the conclusion that SVE ameliorate the cardiac and renal dysfunction.
  Hasan S. Yusufoglu , Gamal A. Soliman , Ahmed I. Foudah , Maged S. Abdulkader , Hossny A. El-Banna , Aftab Alam and Mohammad Ayman Salkini
  Background and Objective: Arnebia hispidissima (F. Boraginaceae) has been found to have cardiac and febrifuge properties. It has long been used in traditional system of medicine for the treatment of heart ailments, headache and fever. This study aimed to investigate the protective effect of Arnebia hispidissima extract (AHE) on carbon tetrachloride (CCl4)-induced cardiotoxicity and nephrotoxicity. Materials and Methods: Adult rats were divided into five groups and medicated as follows: (1) The control group received the vehicles (olive oil; 1 mL kg–1, i.p.+3% Tween 80; 1 mL kg–1, p.o.). (2) CCl4 group of animals was administered with 20% CCl4 in olive oil (1 mL kg–1, p.o.). (3) Silymarin group was co-administered with CCl4 plus silymarin (50 mg kg–1, p.o.). (4) CCl4 plus AHE (200 mg kg–1, p.o.). (5) CCl4 plus AHE (400 mg kg–1, p.o). Rats received vehicle, CCl4, silymarin or AHE twice a week for 8 weeks. Serum AST (aspartate transaminase), LDH (lactate dehydrogenase), CK (creatine kinase), CK-MB (creatine kinase-MB isoenzyme) and cTnI (cardiac troponin) were measured to assess the heart damage markers. Serum levels of creatinine, urea, uric acid and BUN (blood urea nitrogen) were measured as markers of the renal function. Markers of oxidative stress in the cardiac and renal tissues were estimated by determining the levels of SOD (superoxide dismutase), GPx (glutathione peroxidase), CAT (catalase), GSH (reduced glutathione) and MDA (malondialdehyde). Heart and kidney tissues were investigated for histopathological changes. Results: Administration of CCl4 significantly increased the levels of cardiac and renal damage markers. Co-administration of CCl4 plus AHE significantly relieved the adverse effect of CCl4 in rat and reduced the increased serum levels of cardiac and renal damage markers. AHE compensated the deficits in the antioxidant defense mechanisms (SOD, GPx and CAT) and suppressed LPO (lipid peroxidation) in rat heart and kidney resulting from CCl4 administration. Moreover, histopathological changes induced with CCl4 in heart and kidney tissues of rat were also reduced with the co-administration of AHE. Conclusion: In this study, we have found that oral administration of AHE prevented CCl4-induced cardio- and nephrotoxicity by accelerating heart and kidney antioxidant defense mechanisms and down regulating the LPO near to the normal levels.
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