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Articles by Afaque Quraishi
Total Records ( 2 ) for Afaque Quraishi
  Afaque Quraishi , Shailesh Kumar Jadhav and Sheetal Gupta
  Cassia tora L. is a leguminous annual herb of tropical part of India. It is an important medicinal plant with commercially valuable seeds. Micropropagation of this medicinal legume via bud culture method has been first time reported here. Nodal explants from field grown plants were used to initiate in vitro culture. Heavy black leaching was observed in initiation medium from cut ends of the explants and all the explants were eventually died. Leaching was effectively checked by incorporation of an absorbent and an antioxidant -25 μM polyvinyl pyrrolidone -40 and 476 μM citric acid in the initiation medium, respectively. Best 90% shoot bud initiation with 1.5±0.1 shoots per explant was recorded on MS medium supplemented with 2.2 μM 6-benzyl adenine. Elongated shoots from original explants were divided into nodes and best shoot production was observed on MS basal medium, which also induced roots from the propagule. These shoots on the basal medium were longest (3.3 cm±0.38) also with highest number of nodes. There was no leaching observed at this stage therefore the mixture of polyvinyl pyrrolidone -40 and citric acid was not incorporated into the medium. For better quality roots and 100% rooting in shoots, elongated shoots were transferred into ½ strength MS medium supplemented with 2.5 or 4.9 μM indole-3-butyric acid. Regenerated plantlets were successfully hardened off in greenhouse with 70% survival.
  Ravishankar Chauhan , S.K. Jadhav and Afaque Quraishi
  In vitro germination of hybrid seeds of papaya (Carica papaya Linn.) was attempted in the present investigation. Hybrid papaya seeds were subjected to three kinds of treatments. First on semisolid medium Murashige and Skoog (1962), secondly on only inorganic salt solution of the same medium but without organic nutrients and with various combinations of plant growth regulators on coco-peat as support material and in the third set of experiment, the seeds without seed-coat with 6-benzyl adenine having coco-peat as support material. Results showed that the germination rate of the papaya seeds was inhibited by the seed coat. Therefore, the best 90% in vitro germination was observed when the seeds without seed-coat were subjected to 0.5 mg L-1 6-benzyl adenine in coco-peat containing vessels. The seedlings were subjected to in vitro hardening. After three months of transfer to greenhouse and subsequently in nursery, the young seedlings showed 90% survival.
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