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Articles by Adela PINTEA
Total Records ( 9 ) for Adela PINTEA
  Mirela JIMBOREAN , Cornel LASLO , Dorin TIBULCA , Constantin BELE , Adela PINTEA , Adriana PAUCEAN and Aurora TIBULCA
  The aim of the present paper is to study the variation of fatty acids content during the ripening of semihard cheese (type Holland), after adding in the milk, used for the coagulation, of a lipolytic enzymes, and the comparison with the data obtained from the analysis of a witness cheese (without enzymes added) obtained and ripen in the same technological conditions. For investigations were collected and analyzed samples at different times during ripening.
  Cristian Tudor MATEA , Maria -Loredana SORAN , Adela PINTEA and Constantin BELE
  The analysis of carotenoids presents in inland Ocimum basilicum L. irradiated in a microwave field was performed in this study. The extracts of Ocimum basilicum L. were obtained with Ultraturrax in water: acetone (10 + 90, v/v) mixture, stirring 2 h. The extracts were analysed using a Jasco V-530 UV-VIS spectrophotometer and a Shimadzu HPLC equipped with PDA detector, for carotenoids identifying. The HPLC analyses were performed on YMC C30 column, 5 μm, 250 x 4.6 mm, using a linear gradient of two different solvents mixtures A - methanol : methyl-tert-butyl-ether : water (81+15+4, v/v) and B with the sames component but in the follow range 6+90+4, v/v were the solvent mixtures used as mobile phase. The linear gradient was 1%B to 100%B in 90 min. The carotenoids identification was performed using the standard solutions.
  Carmen SOCACIU , Monica TRIF , Dumitrita RUGINA , Adela PINTEA and Simion ASTILEAN
  We aimed to demonstrate that the functionalization of nanocolloids of Au in vitro, induce the formation of conjugated forms (with glutathion) which can affect the cellular activity, as tested on Saccharomyces cerevisiae and RPE cells. The cell line relevant for macular degeneration, type RPE (line D407) proved to be more sensitive to nanogold and conjugated forms. By microscopy we demonstrated the cell capacity to form a monolayer, as a prove of their proliferation and viability, as well in the presence of free AuC, glutathione, but also in the presence of conjugated forms AuC-glutathion. AuC-glutathion conjugate, at pH 5.6 (optimum for cell growth) is stable and can have effect on cellular activity, with impact on proliferation and viability. Obviously, the interaction of AuC and AuC-glutation conjugate with cell components may have effects on cell proliferation and rapid metabolization in presence of appropriate enzymes. (e.g. Glutation peroxidase). Alternative and complementary studies are needed to show the localization of AuC and AuC-glutathion conjugate at cellular level, by microscopy and spectroscopy. It is possible that, inside cell, AuC to conjugate other biomolecules, with a higher stability and affinity, comparing to glutathion.
  Francisc DULF , Sanda ANDREI , Cristian MATEA , Andrea BUNEA and Adela PINTEA
  The aim of the present study was to investigate the fatty acids, sterols and chlorophylls content and profile in leaves of apple cultivars (Malus domestica Borkh) which are intended to be cryopreserved. Quantitative GC-FID was used for phytosterols and fatty acids analysis, while chlorophylls were determined by a spectrophotometric method. Total phytosterols ranged from 189.6 to 463 mg/total lipid extract. The highest phytosterols content was found in Romus 3 and Romus 4 cultivars. The most important phytosterols in all leaves samples were: β-sitosterol, sitostanol, Δ5-avenasterol, 24-methylenecycloartenol and Δ7-stigmastenol. Some other minor sterols were also identified: campesterol, stigmasterol, citrostadienol. Fatty acids analysis revealed important quantitative differences, with a total content ranging from 77.4 mg/g to 245 mg/g. The major fatty acid in all samples was α-linolenic acid with 40 - 55 %, followed by palmitic acid with 17.5 - 25 %, linoleic acid 7 - 14 % and oleic acid 4.7 - 9.6 % of total fatty acids. Polyunsaturated fatty acids represent the major fraction, comprising 54-65 %. The ration between unsaturated and saturated fatty acids ranged between 2.1 - 3.2. The high degree of unsaturation is characteristic to green tissues. The chlorophyll a content ranged between 1.39 - 2.3 mg/g fresh weight, while chlorophyll b was found at 0.6 - 0.98 mg/g. The ratio chl a/chl b was close to 2.3 for all apple cultivars.
  Andreea BUNEA , Augusta LUJERDEAN , Adela PINTEA , Sanda ANDREI , Claudiu BUNEA and Constantin BELE
  The aim of this study was to extract the carotenoids from the marigold flowers and to use cellulases, hemicellulases and pectinases from the beginning of the extraction for better extraction. The concentration of the enzymes used was different also the time of hydrolysis. Total carotenoid content was measured using the spectrophotometric method. HPLC-DAD was used to determine the carotenoid profile for the extracts. HPLC analysis of the extract indicates that the original carotenoid profile is not altered. The enhanced extraction system appears to be very competitive when compared to the classical extraction using solvents.
  Dumitrita RUGINA , Simona VICAS , Mariana PETRAN , Adela PINTEA , Andrea BUNEA and Carmen SOCACIU
  In Europe, mistletoe extract (Viscum album) is widely used as complementary cancer therapies. The main active component of mistletoe extract is glycoprotein, lectin, that exhibit cytotoxic effects and immunomodulatory activities. The aim of this study was to show if the mistletoe extract, rich in phenolic acids presents anti-carcinogenic potential on A2780 tumor ovarian cells. In the first part of this study, using high performance liquid chromatography (HPLC) analysis we characterized phenolic acid content of mistletoe growing on Malus domestica. And, in the second part, we investigated whether by apoptosis on ovarian tumor cell line A2780 if anti-cancer effect of this extract mistletoe is induced. Cell proliferation was assessed by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenylt-etrazolium bromide (MTT) assay. The A2780 cell viability was inhibited to 87% by 50 μg/ml mistletoe extract treatment, and to 79% at 110 μg/ml extract concentration. To find out the apoptotic cell death, 4, 6-diamidino-2-phenylindole (DAPI) staining was performed. Cells treated with mistletoe (110 μg/ml) for 24 h displayed typical morphological features of apoptotic cells, with condensed and fragmented nuclei. Fluorimetric method was used to evaluate total reactive oxygen species content from A2780 ovarian tumor cells after mistletoe treatment. Mistletoe concentration 70μg/ml induces a significant toxic ROS-generating effect. These data indicates that the phenolic acids from mistletoe induce cell death by apoptosis on human tumor ovarian cell line, A2780.
  Daniel COCAN , Elena HORJ , Monica CULEA , Vioara MIRESAN and Adela PINTEA
  In this study, we aimed to determine blood plasma amino acids concentration of rainbow trout (Oncorhynchus mykiss) in two different seasons (spring and summer). For this, we collected blood samples from specimens of rainbow trout with body weight between 200 and 250 grams. Specimens studied, both in the spring season and in the summer, were randomly collected from breeding pools, and were part of the same batch. Growth and feeding conditions were the same for all specimenns studied. The essential amino acids determined were: threonine (Thr), valine (Val), leucine (Leu), isoleucine (Ile), methionine (Met), phenylalanine (Phe), tyrosine (Tyr) and lisine (Lys). Non-essential amino acids determined was: alanine (Ala), glycine (Gly), serine (Ser), proline (Pro), hydroxiproline (Hy-pro), ornithine (Orn), aspartic acid (Asp) and glutamic acid (Glu). Determination of amino acids concentration was performed by gas-chromatography technique and interpretation of results was performed by established statistical methods. For most amino acids, their concentration decreased in summer, compared with those recorded in the spring season.
  Daniel COCAN , Elena HORJ , Monica CULEA , Vioara MIRESAN and Adela PINTEA
  In this study, the authors evaluated the changes under the action of environmental factors, on the structure of free fatty acids in blood plasma of rainbow trout (Oncorhynchus mykiss), between spring-summer seasons. Maintenance and feeding conditions were the same for all specimens of rainbow trout studied. Were retained for this purpose, 20 individuals of rainbow trout with body weights between 200 and 250 grams, from which blood samples were collected. Structure determination of free fatty acids in blood plasma of rainbow trout, was performed using gas chromatography technique-mass spectrometry. Eicosapentaenoic acid (EPA) and docosapentaenoic (DHA) are the major fatty acids found in plasma of rainbow trout (Oncorhynchus mykiss). The purpose of this study was to determine the extraction procedure, the method of derivatization, temperature separation program and identification of fatty acids in fish plasma. During summer, there was an increase in strenght for most unsaturated fatty acids.
  Zorita SCONTA , Dumitrita RUGINA , Floricuta RANGA , Adela PINTEA and Andrea BUNEA
  Different Romanian cultivars of Vaccinium corymbosum (Elliot, BlueCrop, and Duke) and Vaccinium myrtillius (Wild 1, Wild 2) were collected and analyzed for chlorogenic acid using High Performance Liquid Chromatography. Bluecrop cultivar was found to have the highest chlorogenic acid content (181.55 μg/100 g FW) among the cultivated blueberries analyzed, but the lowest values were obtained for Duke cultivar (97.84 μg/100 g FW). Both wild cultivars had high quantities of chlorogenic acid comparing to cultivate ones.
 
 
 
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