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Articles by Adel Sharaf Al-Zubairi
Total Records ( 5 ) for Adel Sharaf Al-Zubairi
  Siddig Ibrahim Abdel-Wahab , Ahmad Bustamam Abdul , Hew Kim Fong , Syam Mohan , Manal Mohamed Elhassan , Adel Sharaf Al-Zubairi and Abdelbasit Adam Mariod
  The aim of the present study, is to evaluate the antimicrobial, phenolic content and free radical scavenging properties of the dichloromethane extract of Goniothalamus umbrosus leaves. The antimicrobial activities were evaluated using 2 Gram-positive bacteria, Methicillin Resistant Staphylococcus aureus (MRSA) and Bacillus subtilis B 29 and 2 Gram-negative bacteria, Pseudomonas aeruginosa 60690 and Salmonella choleraesuis using disc diffusion method and compared alongside to streptomycin. Antioxidant effect and total phenolic content of the extract were also measured by DPPH assay and Folin-Ciocalteu reagent, respectively. The results have concluded that the extract explicit a broad spectrum antimicrobial activities against all tested bacteria. However, antioxidant activity is significantly different from the activity of the positive control, BHT. Accordingly, the total phenolic compounds were also, observed to be correlated positively with the low antioxidant activity revealed by the extract. As a conclusion, the promising broad spectrum antimicrobial activities of the dichloromethane extract of the leaves of G. umbrosus might be due a different chemical constituent (s) since, the phenolic compounds were not found richly in the extract. Further, phytochemical investigations are currently conducted to explore the active ingredients as new substance (s) in the pharmaceutical industry.
  Manal Mohamed Elhassan Taha , Siddig Ibrahim Abdel Wahab , Fauziah Othman , Parichehr Hanachi , Ahmad Bustamam Abdul and Adel Sharaf Al-Zubairi
  The aim of the current study is to determine the effects of A. indica aqueous extract on Diethyl Nitrosamine (DEN) and 2-Acetylaminofluorene (AAF) induced-hepatocarcinogenesis on Spraque-Dawley rats. The plant, A. indica, extract was prepared into 5% w/v in distilled water. Spraque-Dawley male rats were divided into 3 groups of 7 rats each. The groups were: DEN/AAF-induced rats (C), DEN/AAF-induced rats treated with 5% A. indica (CAI) and normal control group (N). In situ detection of DNA fragmentation, TUNEL assay, was used to investigate the apoptogenic properties of A. indica. RT-PCR was used to amplify AFP mRNA. TUNEL assay supported that there was more numbers of apoptotic cells in the liver of (CAI) group compared with (C) group. AFP gene was suppressed by the supplementation of A. indica to DEN/AAF rats (CAI). A. indica (Neem) has revealed a chemopreventive capability by regressing the hepatocarcinogenesis induced by DEN/AAF carcinogens. This capability can be seen from the modulating effects of the plant in the biological indicators used in this study.
  Siddig Ibrahim Abdel-Wahab , Ahmad Bustamam Abdul , Syam Murali Mohan , Adel Sharaf Al-Zubairi and Manal Mohamed Elhassan
  This current study, aims to investigate the ethyl acetate and methanol extracts of Goniothalamus umbrosus for their anticancer effects on several human cancer cells namely, the MCF-7 breast cancer, HT-29 colon cancer and CEM-ss leukemia cell lines using a 3 days MTT (3-(4 and 5-dimethylthiazol-2-yl)-2 and 5-diphenyltetrazolium bromide) assay. Morphological changes and probable mode of cancer cell death induced by bioactive G. umbrosus extract were examined. DNA laddering assay was performed to assess endo-nucleosomal fragmentation. The MTT assay results revealed that only the ethyl acetate extract has anticancer effects on human breast cancer cells (MCF-7). Half maximal Inhibitory Concentration (IC50) of the ethyl acetate extract was found to be 24.5±0.12 μg mL-1. Both inverted and fluorescence microscopic studies demonstrated that treated MCF-7 breast cancer cells using IC50 of the extract displayed a number of typical morphological changes. Appearance of membrane blebs, DNA condensation and fragmentation are significant signs of apoptosis, were observed. The above findings suggested that the ethyl acetate extract of Goniothalamus umbrosus has potential therapeutic effect towards human breast cancer cells that requires further investigations in future.
  Syam Mohan , Ahmad Bustamam Abdul , Siddig Ibrahim Abdel Wahab , Adel Sharaf Al-Zubairi , Manal Mohamed Elhassan and Mohamed Yousif
  The antioxidant and antibacterial activity of different extracts from of Typhonium flagelliforme (L.) Blume leaves (family: Araceae) commonly called ‘Rodent Tuber’ was assessed towards different antioxidant models as well as in selected bacteria. None of the extracts showed significant activity against the selected strains. The only exception is hexane extract (2.0±0.15 mm diameter) against Pseudomonas aeruginosa. The positive control, Streptomycin had shown zone of inhibition of 20±1.5, 20±1.3, 23±1.5 and 23±1.0 mm in Methicillin Resistant Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella choleraesuis and Bacillus subtilis, respectively. All the extracts were subjected to screening for their possible antioxidant activity. Two complementary test systems, namely DPPH free radical scavenging and total phenolic compounds, were used for the analysis. The results showed that the inhibitory activity of Dichloromethane (60.7±3.2%) and Methanol (60.1±2.3%) extracts were comparatively commendable inhibition capacity when compared to the positive control BHT (95.3±1.3%). The total phenolic content of Methanol extracts (5.69±0.15 GAE mg g 1 extract) was superior to all other extracts, followed by dichloromethane and ethyl acetate. Considering all the results collectively T. flagelliforme appears to be a promising plant demonstrating antioxidant activity that requires further investigation.
  Ahmad Bustamam Abdul , Siddig Ibrahim Abdel-Wahab , Hew Kim Fong , Syam Morali Mohan , Adel Sharaf Al-Zubairi and Manal Mohamed Elhassan
  This present study aims to investigate whether Goniothalamus umbrosus extract has anticancer effects on several human cancer cells namely, the MCF-7 breast cancer cell line, HT-29 colon cancer cell line and CEM-ss leukemia cell line. Morphological changes and probable mode of cancer cell death induced by these G. umbrosus extract was examined. The dichloromethane plant extract of G. umbrosus was investigated for its cytotoxic activities on the human cancer cells using a 3 days MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The MTT assay results revealed that the extract has anticancer effects on human breast cancer cells (MCF-7). Half maximal inhibitory concentration (IC50) of the hexane extract was found to be 19.5±0.338 mg mL-1 and this concentration was further used in investigations towards MCF-7 human breast cancer cells. Both inverted and fluorescence microscopic studies demonstrated that treated MCF-7 breast cancer cells using IC50 of the extract displayed a number of typical morphological changes. Appearance of membrane blebs, DNA condensation and fragmentation are significant signs of apoptosis. The above findings suggested, that the hexane extract of Goniothalamus umbrosus has potential therapeutic effect towards human breast cancer cells that requires further investigations in future.
 
 
 
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