Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
Articles by Absar-ul Hasnain
Total Records ( 2 ) for Absar-ul Hasnain
  Riaz Ahmad and Absar-ul Hasnain
  With the increasing application of ultrasonics in meat tenderization and processing, physicochemical events initiated by sonic radiation at myofibrillar level and propagated in complex tissue such as meat require a clear understanding. The enormous amount of basic information collected by studying myofibrils, actomyosin or their individual constituents has already clarified intricacies of muscular contraction and, part of such basic information has find application in meat sciences. In this investigation, chicken Natural actomyosin (NAM) has been taken as a simple model to work out some effects of ultrasonication in a concentration range of 0.5 to 1.8 mg protein mL-1. At each concentration, NAM solution in 0.6 M NaCl (2.0 mL) was individually exposed to 20 kHz sonic waves for a total of 10 min. Cooling was maintained by keeping NAM containers in crushed ice and a lag of 5 sec after each 10 sec long sonic burst. Aliquots from each sonicated NAM were subjected to biochemical analyses. Most striking differences were observed in Ca2+-ATPase activity, which displayed a steady decline that corresponded with the decreasing protein concentration. Ultrasonication of NAM for 10 min caused a loss of ~47% of Ca2+-ATPase activity at the highest dilution (0.5 mg mL-1). In the same order of protein concentration, turbidity of ultrasonicated NAM also decreased which denotes increasing transparency. Thus, ATPase and turbidity data demonstrate that due to sonic radiation, interactions among constituents of chicken actomyosin complex alter and these structural changes are devoid of any fragmentation. Under present experimental conditions, SDS-PAGE profiles did not reveal any novel band which could be attributed to ultrasonic fragmentation or proteolytic contamination. The findings also suggest that unlike myofibrils, actomyosin is a model where interactions and substructural changes of constituent polypeptides can be investigated without interference of endogenous muscle proteases.
  Riaz Ahmad , Rakesh B. Pandey , S. Hasan Arif , Nikhat Nabi , Mumtaz Jabeen and Absar-ul Hasnain
  SDS-PAGE profiles of soluble eye lens nuclei proteins of walking catfish, Clarias batrachus (Linn.) revealed the existence of eight distinct polymorphs along the western region of Uttar Pradesh, India. On the basis of their molecular weights (Mr) and isoelectric points (pI), the polypeptides were identified as β- and γ-crystallins. The crystallins characteristically focused between pI values of 5.2 to 8.7 and had molecular weights of 19 to 34 kDa. Statistical analysis of polymorphs within random catches indicated a latitudinal pattern of fish distribution. We propose that the observed distribution pattern is the consequence of limited terrestrial migration of C. batrachus. Nocturnal migration to nearby water bodies is its outstanding characteristic as an air-breathing and walking silurid, specifically during rainy season. Population substructure is genetically recognizable into three zones: upper, central and lower; out of which the latter two are most inhabited. F-value according to ANOVA was 1.095 indicating that at different locations, polymorphs existed in differing frequencies (p = 0.375). Present results thus suggest that with the exception of the flood-prone locations, selection pressures which have limited the output of this threatened catfish have not disturbed the homogeneity of population structure in the investigated region.
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility