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by
Abhinav Dwivedi |
Total Records (
2 ) for
Abhinav Dwivedi |
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Rekha Khandia
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Pratibha Vishwakarma
,
Abhinav Dwivedi
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Anshumala Kujur
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Kuldeep Dhama
and
Ashok Munjal
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Objective: Angiogenesis plays critical and essential role in various physiological processes of animals and humans. Present study reports the potential angiogenic modulatory effects of the two different molybdenum salts [molybdenum trioxide (MoO3) and sodium molybdate dihydrate (Na2MoO4.2H2O)] on chorioallantoic membrane (CAM) of embryonated chicken eggs. Methodology: The three groups of the embryonated chicken eggs (1 control and 2 treated groups) were taken and 200 μL of 0.5 M of each of MoO3 and Na2MoO4.2H2O were directly introduced on to the CAM. The resealed eggs were incubated for 72 h in a humid incubator chamber at 37±1°C. Then the eggs were opened to observe the gross and histopathological alterations for angiogenesis modulation. Results: Gross examination revealed reduced number of secondary and tertiary blood vessels in MoO3 treated group, while Na2MoO4.2H2O treated group showed reduction in number of blood vessels with occasional haemorrhages. Histopathological analysis indicated pro-angiogenic effect of MoO3, with presence of numerous mesodermal blood vessels with normal CAM tissue architecture. However, Chorionic Ectoderm (CE) was absent at few places, with intact CE at most places, indicating requirement of critical dose optimization. The Na2MoO4.2H2O was observed as anti-angiogenic, causing inflammation in CAM tissue with hemorrhage and thus can’t be used for therapeutics. Conclusion: The pro-angiogenic properties of MoO3 can be explored to treat ailments related to insufficient angiogenesis like coronary artery disease, chronic wounds, stroke and myocardial infarction. However, to avoid any kind of side effects, further qualitative and quantitative analysis and critical dose determination is required before going for clinical trials. |
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Rekha Khandia
,
Abhinav Dwivedi
,
Akanksha Sahu
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Pratibha Vishwakarma
,
Arvind Kumar
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Kuldeep Dhama
and
Ashok Munjal
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Background and Objective: Angiogenesis (neovascularization) is an important process of forming new blood vessels, essential for embryonic development, reproduction, wound repair and growth. Present study reports the plausible angiogenic modulatory effects of the cadmium chloride (CdCl2). Cadmium (Cd) is a toxic substance, which have many inhibitory effects but also medicinally important. There are several toxic materials which in low quantity can be used in therapeutic aspects. Keeping in view, the effects of CdCl2 salt were investigated on the process of angiogenesis in chicken chorioallantoic membrane (CAM) model by assessing gross and histopathological alterations. Materials and Methods: The two groups of the embryonated chicken eggs (10 in each group, 1 control and 1 treated group) were taken and 200 μL of 0.5 M of CdCl2 was directly introduced on to the CAM. The resealed eggs were incubated for 72 h in a humid incubator chamber at 37±1°C. Then the eggs were opened to observe the gross and histopathological alterations for angiogenesis modulation. Results: Gross examination of CAM revealed the reduction in the number of secondary and tertiary blood vessels amongst CdCl2 treated group. Histopathological analysis revealed anti-angiogenic effects of CdCl2 due to the less number of blood vessels and presence of breached mesodermal blood vessels, out of which RBCs were oozing out. Occasional abnormal thickening and accumulation of densely arranged cells at chorionic and allantoic sides was also observed. Conclusion: The anti-angiogenic properties of CdCl2 can be explored in the therapeutics of cancer and tumor related disorders. However, to avoid any kind of side effects, further qualitative, quantitative analysis and critical dose determination is required before going for clinical trials. Present investigations revealed the anti-angiogenic effect of cadmium chloride salt on chicken chorioallantoic membrane. |
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