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Articles by Abdulrahman A.I. AL-Yahya
Total Records ( 2 ) for Abdulrahman A.I. AL-Yahya
  Abdulrahman A.I. AL-Yahya
  Myrrh (Commiphora molmol Engl.) is a medicinal herb widely used in different herbal preparations and is reported to possess several pharmacological effects including antidiabetic action. The present study determines the effect of myrrh, glibenclamide and the effect of co-administration of myrrh with glibenclamide on organ damage due to diabetes in rats. Myrrh was administered at two different doses of 500 and 1000 mg kg–1 orally, while glibenclamide was given at a dose of 0.6 mg kg–1 orally. Diabetes was induced using alloxan using method for induction of type 2 diabetes in rats. The blood glucose levels were determined to confirm the induction of diabetes and just before sacrificing the animals. All the treatments were given for a period of two weeks after diabetes induction. The animals were sacrificed and the weight of kidney, liver and pancreas were determined. The organs were subjected to histopathological examination. Myrrh at both doses prevented organ damage to varying degrees and augmented the effect of glibenclamide in preventing organ damage. It was concluded that myrrh prevents organ damage in diabetic rats and potentiates the effect of glibenclamide probably due to its antidiabetic and antioxidant effects.
  Mohammed S. Alhussaini , Mohamed Adbo Moslem , Mohammed I. Alghonaim , Abdullah A. Al-Ghanayem , Abdulrahman A.I. AL-Yahya , Hamido M. Hefny and Adbul Moneam Saadabi
  Background: This investigation compared genetic similarities and diversities within and among Cladosporium species populations using the two PCR-based markers; Internal Transcribed Spacer (ITS)-PCR and microsatellite-PCR. Methodology: Nuclear ribosomal DNA internal transcribed spacers have been used to analyze intraspecific and interspecific relationships in various fungi. In the present study, the internal transcribed spacer (ITS)-PCR and microsatellite-PCR were used to identify the genetic diversities in Cladosporium species. Results: The Internal Transcribed Spacer (ITS) was amplified using polymerase chain reaction combining primers ITS4 and ITS5. The PCR products were digested with three restriction enzymes and separated by agarose gel electrophoresis. Restriction patterns generated by CfoI and Msp I and RsaI were unique for most species assayed. The ITS-PCR fingerprinting methods led to a clear differentiation of the isolates at the species level. Fingerprinting profiles generated readily discriminated between each of the 6 species. Cluster analysis further supported this observation and clusters corresponding to each species were readily identified in the dendrograms. Seven microsatellite primers out of eight primers were unable to generate visible DNA fingerprints. Conclusion: Amplification experiments demonstrated that microsatellite primer, T3B and (GTG)5 are technically simple tools for assaying genetic variability in Cladosporium spp.
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