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Articles by Abdulaziz M. Al-Bahrany
Total Records ( 3 ) for Abdulaziz M. Al-Bahrany
  Jameel M. Al-Khayri and Abdulaziz M. Al-Bahrany
  Somatic embryogenesis provides the best method for commercial micropropagation of date palm (Phoenix dactylifera L.); however, a current limitation is the lack of synchronization of developing somatic embryos. The objective of this study was to evaluate the effect of Abscisic Acid (ABA) and polyethylene glycol (PEG) combinations on the synchronization of embryo development in date palm cell suspension. Callus maintained on MS medium containing 54 μM Naphthalene Acetic Acid (NAA) and 7 μM 2-isopentenyladenine (2iP) was transferred to regeneration liquid medium supplemented with ABA at 0-100 μM and PEG at 0-15%. Maximum fresh culture weight was obtained with 10% PEG in the absence of ABA. The addition of as low as 1 μM ABA to the suspensions inhibited growth. In the absence of ABA, increasing PEG concentration increased total somatic embryo numbers reaching a maximum number at 10% PEG. Various embryo sizes differing in abundance were associated with different treatments. The highest percentage of medium size embryos, 52%, was obtained at 10 μM ABA; whereas, the highest percentage of small embryos was obtained at 50-100 μM ABA. The small embryos exhibited a state of synchronization. Although, treating suspensions with ABA and PEG affected embryo size distribution, germination was influenced by embryo developmental phase, expressed in size. Germination of 43, 63, 52 and 23% was obtained from the small, medium, large and very large embryos, respectively. Retardation of somatic embryo development caused by ABA can be further exploited to optimize culture synchronization.
  Abdulaziz M. Al-Bahrany
  A comparison of the chemical and fatty acid composition between cvs. Hassawi rice (Oryza sativa L.) and Hassa No. 2 was made. Although other parameters of proximate analysis were not significantly modified, content of crude protein in cv. Hassa No. 2 decreased from 10.68% to 9.24%, whereas carbohydrates increased from 75.69% to 77.38%. In response to breeding, mineral composition was unchanged. Saturated fatty acids content significantly decreased with cv. Hassa No. 2, whereas, unsaturated fatty acids increased in comparison with cv. Hassawi. Palmitic, oleic, and linoleic acids were the most abundant fatty acids in both genotypes. Linolenic acid was significantly higher and palmitic acid was significantly lower in cv. Hassa No. 2 than in cv. Hassawi but myristic, stearic, oleic, and linoleic acid content were comparable. Apparently modifications were detected in the concentration of chemical constituents in seeds of Hassawi rice hybrid, the extent of which was dependent on the parameter being considered.
  Abdulaziz M. Al-Bahrany
  The responses of Hassawi rice (Oryza sativa L.) callus was studied to varying degrees of polyethylene glycol (PEG)-induced water stress including callus growth, water content and proline accumulation. To characterize callus growth in response to PEG, 2.5 g embryogenic callus was grown in 125 ml flasks containing 50 ml each of liquid MS medium supplemented with PEG (MW 8000) at 0, 50, 100, 150, 200, 250 and 300 g l–1. Results revealed that increasing water stress induced by increasing concentration of PEG caused a progressive reduction in callus fresh weight. Significant reduction in callus weight was observed in response to 50 g l–1 PEG, but the inhibitory concentration was identified to be 200 g l–1. Increasing PEG concentration was also associated with a progressive reduction in callus water content, which caused increase in proline accumulation reaching significant increase over the control at 100 g l–1 PEG. This study serves as a precursor for genetic improvement efforts to enhance the tolerance of Hassawi rice to water stress.
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