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Articles by Abdul Rahman Omar
Total Records ( 2 ) for Abdul Rahman Omar
  Faez Firdaus Jesse Abdullah , Abdinasir Yusuf Osman , Lawan Adamu , Mohd Syamil Mohd Yusof , Abdul Rahman Omar , Abdul Aziz Saharee , Abd Wahid Haron , Rasedee Abdullah and Mohd Zamri-Saad
  Haemorrhagic Septicaemia (HS) is an acute, fatal, septicaemic disease of cattle and water buffaloes caused by Pasteurella multocida, serotype B:2 in tropical countries. The limitations associated with accurate predictions of mortality, survival levels and the detection of the presence of the organism from various organs of infected animals. Hence, this study used mouse model to evaluate the pattern of mortality and bacterial recovery from organs. Twenty-four mice were randomly divided into two groups. Infected group were inoculated orally with 109 colony forming unit of P. multocida type B, the group 2 were negative controls. The mice were observed for 5 days post-inoculation. At necropsy, visceral organs of dead animals were subjected for the confirmation using Polymerase Chain Reaction (PCR). The results showed that mortality rate was significantly different (p<0.05) between the infected and control groups. Within infected group, highly significant difference (p<0.05) was observed where 12.5% of the mortality rate was recorded within 24 h and 62.5% within 48 h post-infection. The survival rate, in infected group, was found to be around 25%. In diagnosis, P. multocida type B was detected from all organs of animals that did not survive. In contrast, P. multocida type B was neither recovered nor detected from the organs of mice which survived until the end of the experimental period (120 h). The results of this study indicated that manipulation of the organism in experimental animals provided clear information of the incidence of the disease in the field.
  Zahraa Faisal , Aini Ideris , Abdul Rahman Omar , Mohd Hair-Bejo and Tan Ching Giap
  A total of 571 Mycoplasma gallisepticum (MG) field isolates originated from progenies and commercial poultry farms in Malaysia and 7 reference and vaccine strains were characterized by amplification of selected gene target specific sequences to MG pMGA and pvpA genes using conventional PCR of sequence specific primers. A total of 281 MG positive field isolates out of 571 MG samples were detected with the primer targeted pMGA gene and a total of 188 MG positive field isolates out of 571 MG samples were detected with the primer targeted pvpA gene. Similar and identical banding pattern among MG isolates obtained from progenies samples however, there was a variable on the banding pattern among MG isolates obtained from commercial chickens using the agarose gel electrophoresis. The sequencing analysis results of MG based on selected genes targeted specific sequences were obtained. The genetic diversity of the pMGA and pvpA genes of MG field isolates detected in progenies and commercial chickens were investigated. The gene size variation patterns of the pMGA and pvpA genes among MG field isolates shared identical variations with the pathogenic reference and vaccine strains that is an insertion bp fragments by using the pMGA gene primer set and a deletion bp fragments by using the pvpA gene primer set. However, the gene size variation patterns are quite different from the variation pattern of the less pathogenic vaccine strain that can’t be transmitted vertically. The polymorphism pattern of the primer for pMGA gene might be considered as a pathogenic vertical marker and the polymorphisms patterns of the two primers sets for both pMGA and pvpA genes might be useful for determining the two genetic potential pathogenic marker for MG infection that can differentiate between the highly and the less pathogenic MG isolates.
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