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Articles by Abdul Malik
Total Records ( 5 ) for Abdul Malik
  Abdul Malik
  Background and Objective: Honey is comprise high amount of variety of simple sugars which might serve nutrition to sperm cells. The objective of this study was to evaluate the effects of honey addition into the extender on the quality of frozen thawed in Bali bull spermatozoa. Materials and Methods: A total of four Bali cattle bulls were used in this study. Honey solution was added at the concentration of 0.1, 0.2, 0.3 and 0.4% to bovine semen cryoprotective medium. The cryoprotective extender (skim milk-egg yolk) for the control group was the same as that for the treatment groups except that it was not supplemented with honey solution. Sperm parameters were assessed including motility, abnormality and viability. The data were statistically analyzed pre and post-thawing. Results: The results indicated that percentage of the sperm motility before freezing was significantly lower (p>0.05) among control and treatment groups. Furthermore, the percentage of the abnormality and viability were no significantly different (p>0.05) among control and treatment groups. The sperm abnormality frozen thawed was significantly higher (p<0.05) between control and treatment groups. Whereas, the percentage of the motility and viability of frozen thawed was no significantly different (p>0.05) among control and treatment groups. Conclusion: It is concluded that honey supplementation into the extender was significantly effect on the sperm motility before freezing and sperm abnormality on the frozen thawed.
  Sajid Mehmood , Aziz-Ur- Rahman , Zaheer Ahmad , Nighat Afza , Abdul Malik , Habib Ahmad and Muhammad Iqbal Choudhary
  Monoterpene glycosides have been isolated from the ethyl acetate soluble fraction of Indigofera hetrantha and their structures assigned to be 3,7-dimethyl-2(E),6-octadien-5-one-1-O-β-D-6-O-acetylglucopyranoside (1) and 3,7-dimethyl-2(E), 6-octadien-5-one-1-O-[-β-D-6’’-O-acetylglucopyra-nosyl (1’’ → 6’)-β-D-glucopyranoside] (2), on the basis of spectroscopic analysis including 1D and 2D NMR techniques. Compounds 1 and 2 displayed moderate inhibitory potential against the enzyme lipoxygenase.
  Abdul Wahab , Ejaz Ahmed , Sarfraz Ahmad Nawaz , Ahsan Sharif , Rizwan Ul Haq , Abdul Malik , Muhammad Iqbal Choudhary and Mohsin Raza
  Haloxylon recurvum Bunge ex Boiss (Chenopodiaceae) is distributed chiefly from the Mediterranean region to Central and South Asia and traditionally applied externally for a variety of disorders. We investigated the in vivo toxic potential of crude methanolic extract of the whole plant and its n-hexane, chloroform, butanol, ethylacetate and aqueous soluble fractions by determining their acute toxicity and acute neurotoxicity in mice using Lorke`s method and inverted screen test. In vitro studies were also conducted in order to investigate its antilipoxygenase, antibacterial and antifungal activities. All the fractions showed a narrow margin of safety in mice, except the aqueous fraction, which did not produce any mortality even at the highest tested dose (5000 mg kg-1). At non-lethal doses, only the aqueous fraction (TD50 1264 mg kg-1) was found to produce neurotoxicity in mice. In in vitro lipoxygenase inhibition assay, the ethylacetate fraction showed the most significant inhibitory activity. Crude methanolic extract and its butanol soluble fraction showed the most potent antifungal and antibacterial activity for all the materials tested. Thus, this report verifies the traditionally reported toxicity of this plant, as the majority of its components have exhibited a narrow margin of safety, however, they have been found active in in vitro studies, therefore, further studies are required in order to isolate the most active toxic compounds and differentiate them from these fractions.
  Sri Hidanah , Sunaryo Hadi Warsito , Tri Nurhajati , Widya Paramita Lokapirnasari and Abdul Malik
  Objective: The objective of the present study was to determine the effects of mangosteen peel and ginger rhizome on the performance and cholesterol levels of heat-stressed broiler chickens. Materials and Methods: One hundred unsexed day-old commercial broiler chicks were fed one of four experimental diets. Diet T1, the control treatment, contained neither mangosteen peel nor ginger rhizome, diets T2 and T3 contained 5% mangosteen peel and 5% ginger rhizome, respectively and diet T4 contained 2.5% of both mangosteen peel and ginger rhizome. Results: The results indicated that the consumption of diet T4 was significantly higher than that of the other three diets (p<0.05). The mean live weight of broilers fed diets T2 and T4 was significantly greater (p<0.05) than that of broilers fed the control diet. The abdominal fat ratios and cholesterol levels of broilers fed diets T3 and T4 were significantly higher (p<0.05) than that of broilers fed the control diet. Conclusion: The performance of broiler chickens can be enhanced by the addition of mangosteen peel or a combination of mangosteen peel and ginger rhizome to feed formulations.
  Ranjan Kumar Bhagobaty and Abdul Malik
  In the present study wastewater irrigated agricultural soil with a previous history of chlorpyrifos use was examined for its capacity to harbor bacteria capable of utilizing it as a sole source of carbon. Four bacterial isolates designated as RA-3, RA-5, RA-10, RA-20, isolated from the soil, using enrichment culture technique showed promising capability to utilize chlorpyrifos as a carbon source for their growth. Morphological and biochemical tests performed on the bacteria indicated that they might belong to the genus Pseudomonas. Thin layer chromatography and tetrazolium reduction assay showed that the strains were capable of degrading chlorpyrifos. All the chlorpyrifos degrading bacterial isolates were also tested for their antibiotic sensitivity against 10 antibiotics/drugs. All the isolates were sensitive to gentamycin and methicillin. RA-10 and RA-3 were sensitive to ampicillin whereas RA-5 was resistant and RA-20 showed intermediate range of sensitivity. RA-5 and RA-3 were sensitive to chloramphenicol whereas, RA-10 and RA-20 showed intermediate sensitivity. RA-5 and RA-20 showed resistance against co-trimoxazole and nalidixic acid. RA-5 and RA-10 showed intermediate sensitivity to tetracycline whereas RA-20 was resistant and RA-3 sensitive to it. All the bacterial isolates were also found to harbor a single plasmid. This leads us to believe that the soils with previous exposure to chlorpyrifos contain a diverse range of bacteria having novel organophosphorus hydrolase enzyme systems for causing the enhanced biodegradation of this toxic pesticide in the environment. Further elucidation of the enzymatic and molecular mechanisms involved in the process will help in creating possible bioremediation technologies using the soil bacteria.
 
 
 
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