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Articles by Abdul Hameed
Total Records ( 5 ) for Abdul Hameed
  Ali Abbas Qazilbash , Rani Faryal , Ayesha Tabasum , Asima Lodhi , Safia Ahmad and Abdul Hameed
  Over the years, with the active spread and development of the industries, heavy metals, which are either used, or produced, as by-products, by numerous manufacturing, industrial, refining and mining processes, have become ubiquitous, persistent environmental pollutants. Bioremediation is a cost-effective technology that may be used to remove a variety of pollutants and contaminated sites. This study was designed to compare the capabilities of identified Aspergillus niger strains in the oven-dried (dead) and non-growing (living) forms to adsorb Lead (Pb) from SDB media containing the HM under optimized laboratory conditions using the shake-flask incubation method. Aspergillus niger showed a higher percentage of Pb adsorption in the oven-dried, as compared to the non-growing state. Furthermore, the adsorption by oven-dried strains was also high at higher concentrations of the heavy metal, whereas adsorption of Pb effectively took place at the low concentration levels for the non-growing strains. Analysis of the correlation matrices showed significant positive correlation between strains at high Pb concentrations under the oven-dried condition and significant positive correlation at low Pb concentrations under the non-growing conditions. The percentage Pb adsorption of both strains under oven-dried conditions was over 90% for the concentration range of 100-500 ppm, with the highest being 99.95% at 500 ppm for strain NP 18 after 80 min of incubation. Furthermore, for the non-growing strains this maximum adsorption was achieved for the concentration range of 100-300 ppm and that too was 96.37% achieved by strain NP 17 at 300 ppm concentration after 30 min, after which the toxic effects of the HM on the live fungal strains diminished their capacity to adsorb the metal. When comparing the two biotreatment regimens of oven-dried fungal biomass, verses the use of non-growing pellets of fungi, there is overwhelming evidence to support the use of the former as a method to removal HM contamination from polluted sites, particularly at high concentration levels.
  Bushra Uzair , Abdul Hameed , Sidrah Nazir , Barkat Ali Khan , Fehmida Fasim , Samiullah Khan and Farid Menaa
  Background and Objective: Synergistic combinations of various antimicrobial agents have been introduced as extra successful strategies to combat multidrug resistant (MDR) infections. This study was undertaken to evaluate MDR profiling of Staphylococcus aureus strains isolated from clinical specimens of two public hospitals of twin Pakistani cities as well as to explore in vitro antibacterial potential of Nigella sativa L. (black cumin or black seeds) extract against Mec A gene positive Staphylococcus aureus strains. Materials and Methods: The MDR strains were then screened for positive Mec A gene by PCR and sequencing. A total of 500 bacterial strains were subjected to antimicrobial and Nigella sativa seeds susceptibility and minimal inhibitory concentration assays. The data were evaluated as Mean±Standard deviation of five independent experiments. The results were analyzed using t-test with SPSS version 16.0. Results: Methanolic extracts of Nigella sativa seeds showed maximum activity against Mec A gene positive Staphylococcus aureus alone and in combination with the penicillin antibiotic Augmentin® plus the second-generation Cephamycin, Cephalosporins, Mefoxin® (Cefoxitin). The anti-MRSA activity was reduced when methanolic extracts of Nigella sativa seeds were used in combination with the pain reliever and the fever reducer paracetamol (acetaminophen). Eventually, it has been observed that the anti-staphylococcal activity of Nigella sativa led to changes in bacterial cell morphology indicating that the cell wall of Gram-positive bacteria as likely a target of action. Conclusion: This study provides new insights about synergistic antimicrobial and Nigella sativa crude extract activities against MDR Staphylococcus aureus strains.
  Zaheer Ahmed , Abdul Hameed and Khalid Naeem
  In continuation of our studies with the Infectious Bronchitis prevalence in Pakistan, the current study was conducted to determine the teratological effects of the Infectious Bronchitis Virus (IBV) on developing chicken embryo. The tissues collected (Trachea and Lungs) from clinically positive IB chickens were tested with the indirect immunofluorescence assay against M-41 IBV strain (reported previously). The positive samples were further tested with the RT-PCR against the IBV primers (reported previously). The tissue homogenates from 43 such RT-PCR-positive samples were serially passaged through 11 days old embryos and the effects on the embryonic development in terms of dwarfing, curling, stunting and urates deposits were recorded. The data showed that the passaging sequentially increased the IBV teratological effects. Only 13 out of 43 samples exhibited IBV-associated teratological effects over various passages. These effects were effectively neutralizes by using IBV variant specific antisera in a viral neutralization test. These studies imply that IBV variants isolated from Pakistan are no different in producing teratological effects considered representative of the IBV.
  Sofia Khanum , Khalid Naeem , Zaheer Ahmed , Sadia Sattar and Abdul Hameed
  Intravenous pathogenicity index (IVPI) of three Avian influenza virus (AIV) isolates of serotypes H7N3 was determined at their zero, 10 ,20 and 30th in ovo-passage levels to check the effect of mutation during passage on pathogenicity of the virus. For this purpose three local field isolates of AIV H7N3 were passaged in 9 day old chicken embryonated eggs. Each passaged isolate was subjected to biological evaluation at every 10th passage level. It was found that in-ovo passaging of viruses had both positive and negative effect on pathogenicity index of H7N3 isolates. It was also determined that among three isolates only one isolate showed increase in pathogenicity by passaging while the other two showed decline in their pathogenicity index. On the other hand repeat passaging helped to increase HA titers in all the isolates.
  Abdul Haleem Shah , Abdul Hameed and Gul Majid Khan
  The objective of this investigation was to develop a new auxotrophic mutant from a regulatory mutant and to examine its potency for lysine production. Glutamate-homoserine-alanine triple auxotrophic mutant MRLH-GHA10 was derived from thiosine resistant mutant of Corneybacterium glutamicum. Its potency was examined in various media containing different carbon sources. The most potent auxotrophic mutant developed in this investigation produced 38, 33 and 28.5 g L-1 lysine, in the media containing glucose, molasses and starch hydrolyzate, respectively as carbon sources in a stirred tank fermenter, at 30 °C and at 500 rpm agitation.
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